-
Plasmid#175432PurposeUsed to generate IVT RNA for translation extractsDepositorInsertHBB-nLuc
UseLuciferaseTagsExpressionBacterialMutationPromoterT7Available sinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLentiguide-puro_hORAI1sg
Plasmid#174326PurposegRNA targeting hORAI1 - encodes puromycin resistanceDepositorInserthuman Orai1 (ORAI1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
HS-OS-Plk4-mKate2
Plasmid#105944Purposeheat shock inducible transgenesis, non phoshorylable version of Plk4DepositorInsertPlk4 (Plk4 Mouse)
UseTagsmKate2ExpressionBacterialMutation1002 to 3704 of Plk4/ deletion of S282-S305.PromoterAvailable sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG-CMV-14-Mfn1-T562A
Plasmid#191976PurposeTo produce mutants mimicking dephosphorylated Mfn1DepositorInsertMfn1 T562A (Mfn1 Mouse)
UseTagsExpressionMammalianMutationT562APromoterAvailable sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG-CMV-14-Mfn1-T562D
Plasmid#191977PurposeTo produce mutants mimicking phosphorylated Mfn1DepositorInsertMfn1 T562D (Mfn1 Mouse)
UseTagsExpressionMammalianMutationT562DPromoterAvailable sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
HS-aPKC-CAAX-GFP
Plasmid#105946Purposeheat shock inducible transgenesisDepositorInsertaPKC (Prkcz Rat)
UseTagsCAAX and GFPExpressionBacterialMutationPromoterAvailable sinceFeb. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-LifeAct-FRB-Venus-173-N
Plasmid#87616PurposeFRB-tagged N-terminal Venus (YFP) fragment for BiFC; localizes to actin cytoskeleton.DepositorInsertLifeAct-FRB-Venus173-N
UseTags17-aa sequence from Abp140 (aka "Lifeact&quo…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
-
cmamxA1-GFP
Plasmid#107195PurposeExpresses porcine annexin A1-GFP fusion protein for live cell imaging, all type II Ca2+ binding sites deletedDepositorInsertcmannexin A1
UseTagsGreen Fluorescent ProteinExpressionMammalianMutationexchanges Asp170Ala, Glu255Ala, Glu330Ala, all ty…PromoterCMVAvailable sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG-CMV-14-Mfn1-T564A
Plasmid#191978PurposeTo produce mutants mimicking dephosphorylated Mfn1DepositorInsertMfn1 T564A (Mfn1 Mouse)
UseTagsExpressionMammalianMutationT564APromoterAvailable sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG-CMV-14-Mfn1-T564D
Plasmid#191979PurposeTo produce mutants mimicking phosphorylated Mfn1DepositorInsertMfn1 T564D (Mfn1 Mouse)
UseTagsExpressionMammalianMutationT564DPromoterAvailable sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-LifeAct-FRB-DMVF-181-N
Plasmid#87610PurposeFRB-tagged N-terminal Dronpa fragment for BiFC; localizes to actin cytoskeleton.DepositorInsertLifeAct-FRB-DMVF181-N
UseTags17-aa sequence from Abp140 (aka "Lifeact&quo…ExpressionMammalianMutationPromoterCMVAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-RaichuEV-Rac1(T17N)
Plasmid#164902PurposeExpresses a Rac1 FRET sensor RaichuEV-Rac1 which contains a dominant negative mutation T17N in the Rac1 domainDepositorInsertRaichuEV-Rac1 (RAC1 Human)
UseTagsExpressionMammalianMutationT17N mutation in the Rac1 domain which make the s…PromoterAvailable sinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAFNF-Venus-Actb
Plasmid#169788PurposeAn actin FRET sensor. Must be expressed with ECFP-actin for FRET.DepositorInsertActb (Actb Mouse)
UseTagsVenusExpressionMammalianMutationPromoterCAGAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SMAD2_HAe2_Bsd
Plasmid#167166PurposeBlasticidin selection cassette flanked by homology arms from human SMAD2 exon 2 for use in creating CRISPR-Cas9 mediated gene knockout.DepositorInsertRepair template to knockout SMAD2 by inserting a blasticidin resistance cassette (SMAD2 Human)
UseRepair template for gene editing in human cellsTagsblasticidinExpressionMutationPromoterAvailable sinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
human KANK2-A670V Ankyrin Repeats pET151
Plasmid#164055PurposeExpresses human KANK2 Ank domain - A670V mutant in bacterial cellsDepositorInsertKidney Ankyrin Repeat-Containing Protein 2 A670V mutant (KANK2 Human)
UseTagsHis-tag, TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSicoR-TTF2i2
Plasmid#59623PurposeExpression of shRNA against human TTF2DepositorInsertTTF2 (TTF2 Human)
UseRNAiTagsExpressionMammalianMutationPromoterU6Available sinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSicoR-TTF2i1
Plasmid#59622PurposeExpression of shRNA against human TTF2DepositorInsertTTF2 (TTF2 Human)
UseRNAiTagsExpressionMammalianMutationPromoterU6Available sinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSicoR-RNF40i1
Plasmid#59594PurposeExpression of shRNA against human RNF40DepositorInsertRNF40 (RNF40 Human)
UseRNAiTagsExpressionMammalianMutationPromoterU6Available sinceSept. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCF730
Plasmid#121668PurposeEF1a-ProCas9-Flavi-P2A-PuroDepositorInsertCas9(C)
UseLentiviralTagsExpressionMutationPromoterEF-1a core promoterAvailable sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only