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pcDNA3-Cerulean3-Cerulean3-FLARE-Cameleon
Plasmid#123353PurposeCyan-fluorescent homoFRET/anisotropy-based genetically encoded calcium indicator.DepositorInsertCerulean3-Cerulean3-FLARE-Cameleon
UseTags6xHIS, Cerulean3, T7 tag (gene 10 leader), and Xp…ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-OSF-BECN1
Plasmid#99328PurposeComponent for mammalian expression of recombinant PI3KC3-C1 complexDepositorInsertBeclin 1 (BECN1 Human)
UseTagsStrep-Strep-FlagExpressionMammalianMutationPromoterCMVAvailable sinceAug. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFUW-TetO-SP110
Plasmid#178448Purposedoxycycline-inducible expression of Human SP110 in mammalian cellsDepositorInsertSP110 (SP110 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
gRNA_DNMT3a-T2
Plasmid#41822PurposeExpresses a guide RNA (gRNA) to target DNMT3a (T2 target sequence) for genome engineeringDepositorInsertgRNA_DNMT3a-T2 (DNMT3A Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJan. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
Lenti-sgApc/Cre
Plasmid#89641PurposeExpresses an Apc-targeting gRNA and Cre-recombinaseDepositorInsertgRNA targeting Apc (Apc Mouse)
UseCre/Lox and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFUW-TetO-SUB1
Plasmid#178440Purposedoxycycline-inducible expression of Human SUB1 in mammalian cellsDepositorInsertSUB1 (SUB1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAC8_GWs-Lox (VE5589)
Plasmid#163769PurposeTransfer vector for gene expression to generate recombinant baculoviruses by homologous recombination. Contains expression cassette under the pH promoter.DepositorInsertno insert
UseTagsno tagExpressionInsectMutationPromoterPHAvailable sinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-POLArISact
Plasmid#164970PurposeT7 promotor drives in vitro transcription of POLArISact with a human kozak sequenceDepositorInsertPOLArISact
UseIn vitro transcriptionTagsExpressionMutationPromoterT7Available sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
gRNA_DNMT3b
Plasmid#41823PurposeExpresses a guide RNA (gRNA) to target DNMT3b for genome engineeringDepositorInsertgRNA_DNMT3b (DNMT3B Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJan. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
JN202: pMVP/SB/Blast-DEST
Plasmid#121864PurposepMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ Blast selection cassetteDepositorTypeEmpty backboneUseSleeping beauty transposon, pmvp gateway destinat…TagsExpressionMammalianMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
JPF0419d
Plasmid#124020PurposeEncodes pCAG driving expression of multicistronic Lyn-tagged iRFP713, cytoplasmic mAzamiGreen, mCerulean-tethered p38 KTR, and Histone 2B fused to mScarlet in a PiggyBac destination vectorDepositorInsertPB_pCAG-Lyn-iRFP713-P2A-NES-mAzamiGreen-P2A-p38KTR-mCerulean-P2A-H2B::mScarlet
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti6/V5-DEST-UCHL5
Plasmid#31219DepositorInsertUCHL5 (UCHL5 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceAug. 19, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-PdTK-CAG-mCh-[uBglII]
Plasmid#107280Purposepuro-deltaTK gene-trap selection cassette (AAVS1 donor vector backbone) with constitutive mCherryDepositorInsertAAVS1 puro-deltaTK; CAG-mCherry (PPP1R12C )
UseTagsExpressionMutationPromoterAvailable sinceMarch 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
JI401: Ad5 E1A/Hexon qRT-PCR standard
Plasmid#131753PurposePlasmid standard containing regions of the Ad5 E1A and Ad5 Hexon genes to create a qRT-PCR standard curve for detection of replication competent adenovirus (RCA).DepositorInsertsAd5 E1A gene fragment
Ad5 Hexon gene fragment
UseStandard for qrt-pcrTagsExpressionMutationPromoterN/AAvailable sinceOct. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSR357.9
Plasmid#125105PurposeExpression of narL(1-129aa)-ydfI(124-213aa) chimera under PLtetO-1, output Promoter PydfJ115DepositorInsertsnarL(REC)-ydfI(DBD)129
sfgfp
tetR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
CXADR_pCSdest
Plasmid#53853DepositorInsertCXADR (CXADR Human)
UseTagsExpressionMammalianMutationPromoterSP6 and CMVAvailable sinceMarch 27, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFUW-TetO-MXD1
Plasmid#178443Purposedoxycycline-inducible expression of Human MXD1 in mammalian cellsDepositorInsertMXD1 (MXD1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLQ2827 pHR: U6-SpsgSV40 CMV-PYL1-KRAB-IRES-mCherry
Plasmid#84260PurposeExpresses Sp sgSV40 gRNA with ABA-inducible KRAB and mCherry for diametric regulationDepositorInsertsSp sgSV40
PYL1-KRAB
UseCRISPR and LentiviralTagsIRES-mCherry and PYL1ExpressionMammalianMutationPromoterCMV and mouse U6Available sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)mGAT1-0-GFP
Plasmid#41662DepositorInsertMus musculus GABA transporter 1 (Slc6a1 Mouse, Synthetic)
UseTagsGFPExpressionMammalianMutationC-terminal hydrophobic isoleucine residue added a…PromoterCMVAvailable sinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits only