Skip to main content
Addgene
Showing: 2981 - 3000 of 3099 results

  3. pNAS1b split mCherry NEDD4 WW2 pos ctrl

    Plasmid
    #112029
    Purpose
    Mode #2 phosphosite positive control (split mCherry, using NEDD4 WW2 binding domain paired with proline-rich positive control peptide)
    Depositor
    Jesse Rinehart
    Article
    Barber et al Nat Biotechnol. 2018 Aug;36(7):638-644.
    Insert
    NEDD4 WW2 domain fused to C-terminal split mCherry, proline-rich pos ctrl interacting peptide fused to N-terminal split mCherry
    Use
    Tags
    Split mCherry
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    112029-plasmid-map-sequence-id-213508

    Enlarge

  4. pCIBN-TetR-tagRFP-T

    Plasmid
    #103809
    Purpose
    'Localizer' construct that marks tetO arrays and is targeted by a PHR-tagged effector upon illumination with blue light; can be visualized without triggering PHR recruitment
    Depositor
    Karsten Rippe
    Article
    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422
    Insert
    CIBN-TetR-tagRFP-T (CIB1, tetR Mustard Weed, E. coli)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    TetR: A4G (M2V)
    Promoter
    CMV
    Available since
    Jan. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    103809-plasmid-map-sequence-id-199261

    Enlarge

  8. pEABR NA 3C FLAG SA

    Plasmid
    #234987
    Purpose
    For production of Extracellular Vesicles (EVs), with the transmembrane region of Neuraminidase protein fused to Streptavidin on the vesicles surface
    Depositor
    Daniel Leahy, Monica Gonzalez-Magaldi
    Article
    Gonzalez-Magaldi et al bioRxiv [Preprint]. 2024 Nov 28:2024.11.
    Insert
    EABR-NA
    Use
    Tags
    HRV 3C site, FLAG, Strep-Tactin (SA)
    Expression
    Mammalian
    Mutation
    In the Streptavidin coding sequence Glu44Val/Ser4…
    Promoter
    Available since
    April 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    234987-plasmid-map-sequence-id-468750

    Enlarge

  10. Cav2.2 BBS W391A pcDNA3

    Plasmid
    #206077
    Purpose
    Cav2.2 calcium channel with an exofacial double Bungarotoxin Binding Site motif in domain II and a mutation (W391A) in the ?1-interacting domain (AID) in the I-II loop that prevents Beta binding
    Depositor
    Annette Dolphin
    Article
    Cassidy et al Proc Natl Acad Sci U S A. 2014 Jun 17;11
    Insert
    cacna1b (CACNA1B Rabbit)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    W391A
    Promoter
    CMV
    Available since
    Sept. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    206077-plasmid-map-sequence-id-406768

    Enlarge

  11. pIRESneo2 mycBioID-Tollip M240A,F241A

    Plasmid
    #178055
    Purpose
    myc-BioID fused to human Tollip carrying CUE domain mutations cloned into the pIRESneo2 plasmid. For generation of stable expressing mammalian cells used for proximity dependent biotin labelling.
    Depositor
    David Tumbarello
    Article
    Ryan et al EMBO J. 2020 Jun 2;39(11):e102539. doi:
    Insert
    Toll-Interacting Protein (TOLLIP Human)
    Use
    Tags
    mycBioID
    Expression
    Mammalian
    Mutation
    Changed Methionine 240 to Alanine and changed Phe…
    Promoter
    CMV
    Available since
    Dec. 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    178055-plasmid-map-sequence-id-350118

    Enlarge

  12. pcdna3 T7 Jip1b(471-707)(C478S, C667S and C699T)

    Plasmid
    #52129
    Purpose
    a mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.
    Depositor
    Roger Davis
    Article
    Whitmarsh et al Science. 1998 Sep 11;281(5383):1671-4.
    Insert
    Jnk interacting protien (Jip1b)(471-707)(M1+2+6) (Mapk8ip1 Mouse)
    Use
    Tags
    T7
    Expression
    Mammalian
    Mutation
    deleted amino acids 1-470 and changed C478S, C667…
    Promoter
    Available since
    June 11, 2014
    Availability
    Academic Institutions and Nonprofits only

  13. pcdna3 T7 Jip1b(471-707)(C478S, C643S and C696T)

    Plasmid
    #52130
    Purpose
    a mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.
    Depositor
    Roger Davis
    Article
    Whitmarsh et al Science. 1998 Sep 11;281(5383):1671-4.
    Insert
    Jnk interacting protein Jip1b(471-707)(M1+3+6) (Mapk8ip1 Mouse)
    Use
    Tags
    T7
    Expression
    Mammalian
    Mutation
    deleted amino acids 1-470 and changed C478S, C643…
    Promoter
    Available since
    June 11, 2014
    Availability
    Academic Institutions and Nonprofits only

  14. pcdna3 T7 Jip1b(471-707)(C478S, C631S and C699T)

    Plasmid
    #52131
    Purpose
    a mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.
    Depositor
    Roger Davis
    Article
    Whitmarsh et al Science. 1998 Sep 11;281(5383):1671-4.
    Insert
    Jnk interacting protein (Jip1b)(471-707)(M1+4+6) (Mapk8ip1 Mouse)
    Use
    Tags
    T7
    Expression
    Mammalian
    Mutation
    deleted amino acids 1-470 and changed C478S, C631…
    Promoter
    Available since
    June 11, 2014
    Availability
    Academic Institutions and Nonprofits only

  15. pcdna3 T7 Jip1b(471-707)(C478S, C631S, C643S, C667S and C699T)

    Plasmid
    #52133
    Purpose
    a mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.
    Depositor
    Roger Davis
    Article
    Whitmarsh et al Science. 1998 Sep 11;281(5383):1671-4.
    Insert
    JNK interacting protein-1b (JIP-1b)(471-707)(M1-5) (Mapk8ip1 Mouse)
    Use
    Tags
    T7
    Expression
    Mammalian
    Mutation
    deleted amino acid 1-470 and C478S, C631S, C643S,…
    Promoter
    Available since
    June 11, 2014
    Availability
    Academic Institutions and Nonprofits only

  16. pcdna3 T7 Jip1b(282-707)(C605S, C631S, C643S and C696T)

    Plasmid
    #52134
    Purpose
    a mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.
    Depositor
    Roger Davis
    Article
    Whitmarsh et al Science. 1998 Sep 11;281(5383):1671-4.
    Insert
    JNK interacting protein-1b (JIP-1b)(282-707)(M1-5) (Mapk8ip1 Mouse)
    Use
    Tags
    T7
    Expression
    Mammalian
    Mutation
    deleted amino acid 1-281 and C605S, C631S, C643S …
    Promoter
    Available since
    June 11, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 2981 - 3000 of 3099 results