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Plasmid#105606PurposeExpresses GFP under PrpsLDepositorInsertGFP
UseTagsExpressionBacterialMutationPromoterPrpsLAvailable sinceMay 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSIBR004
Plasmid#177667PurposeSIBR Intron 4 (Int4) interrupts the CDS of FnCas12aDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterlacUV5Available sinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDrVSP(C302S)-IRES2-EGFP
Plasmid#80331PurposeExpresses Danio rerio VSP (without phosphatase activity) with separated EGFP in mammalian cellDepositorInsertDaino rerio voltage-sensing phosphatase (tpte Zebrafish)
UseTagsExpressionMammalianMutationC302SPromoterCMVAvailable sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSCAR_Cas9-blast_GFP
Plasmid#162074Purpose3rd generation lentivirus transfer vector for Cre-removable Cas9, GFP reporter, and blast resistanceDepositorInsertsCas9-2a-blasticidin S deaminase
EGFP
UseCRISPR, Cre/Lox, and LentiviralTagsExpressionMammalianMutationcodon-optimized for expression in M. musculusPromoterEF1a and sv40Available sinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-FLAG-SF3B1-WT
Plasmid#82576PurposeExpresses a codon-optimized ORF of human SF3B1 (wild-type)DepositorInserthuman SF3B1-WT (SF3B1 Human)
UseTagsFLAG peptideExpressionMammalianMutationPromoterCMVAvailable sinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRS316-4M5.3
Plasmid#45831DepositorInsert4M5.3
UseTagsFLAGExpressionYeastMutationPromoterGAL1Available sinceJune 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLV-mCherry
Plasmid#36084Purpose3rd generation lentiviral plasmid expressing mCherryDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 3xFLAG TPP1 88-544
Plasmid#53548Purposemammalian expression of TPP1 full lengthDepositorInsertTPP1 (ACD Human)
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceMay 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-MtAcKRS (human-opti)
Plasmid#164195PurposeTo express a Methanosarcina thermophila derived AcKRS in mammalian cells for genetic code expansionDepositorInsertMtAcKRS-478(human-opti)
UseSynthetic BiologyTagsExpressionMammalianMutationD76G; L325M; L329I; Y330F; L333A; C372FPromoterCMVAvailable sinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAV pEF1a-DIO-FLPo-WPRE-hGHpA (AAV9)
Viral Prep#87306-AAV9PurposeReady-to-use AAV9 particles produced from AAV pEF1a-DIO-FLPo-WPRE-hGHpA (#87306). In addition to the viral particles, you will also receive purified AAV pEF1a-DIO-FLPo-WPRE-hGHpA plasmid DNA. EF1a-driven, Cre dependent expression of the site-specific recombinase FlpO. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsNoneAvailable sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
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E7 in pENTR1A (w186-1)
Plasmid#22254DepositorInsertHuman papilloma virus E7 (E7 )
UseEntry vectorTagsExpressionMutationPromoterAvailable sinceDec. 17, 2009AvailabilityAcademic Institutions and Nonprofits only -
pBAD_Ub11TCA
Plasmid#174517PurposeRecoded ubiquitin reporter with TCA replacing codon 11 of wt ubiquitinDepositorInsertUbiquitin
UseTags6xHisExpressionBacterialMutationRecoded with TCA replacing codon 11 of wt ubiquit…PromoteraraBADAvailable sinceSept. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-p23
Plasmid#108224PurposeMammalian expression vector for mCherry fusion to human p23DepositorInsertmCherry-p23 (PTGES3 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAS1-rab3P::his-24::mNeptune
Plasmid#124350PurposeNuclear Localized Pan-Neuronal mNeptuneDepositorInsertrab-3, mNeptune (rab-3 Nematode)
UseTagsExpressionWormMutationPromoterAvailable sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCoofy56
Plasmid#122008PurposeMammalian vector for parallel SLIC cloning containing N-terminal TwinStrep and PreScission Cleavage siteDepositorTypeEmpty backboneUseTagsHis10/none and TwinStrepExpressionMammalianMutationPromoterAvailable sinceMay 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
TKTL1
Plasmid#72419PurposeStudy the role of aberrant expression of TKTL1 in HNSCC tumorigenesisDepositorInsertTKTL1 (TKTL1 Human)
UseTagsExpressionMammalianMutationmay have one or two synonymous substitutionsPromoterCMVAvailable sinceApril 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSCM167.2-Venus-VF-hADAM10(aa1-213)
Plasmid#189554PurposeProtein expression ADAM10 in mammalian cells. Contains only prodomain--amino acids 1-213DepositorInserttruncated ADAM metallopeptidase domain 10 (ADAM10 Human)
UseTagsVFExpressionMammalianMutationcontains aa 1-213PromoterAvailable sinceFeb. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSCM167-Venus-VF-hADAM10(aa220-654)
Plasmid#189560PurposeProtein expression ADAM10 in mammalian cells. Contains metalloprotease, disintegrin, and cysteine rich domains--amino acids 220-654DepositorInserttruncated ADAM metallopeptidase domain 10 (ADAM10 Human)
UseTagsVFExpressionMammalianMutationcontains aa 220-654PromoterAvailable sinceFeb. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSCM167-Venus-VF-hADAM10(aa1-459)
Plasmid#189559PurposeProtein expression ADAM10 in mammalian cells. Contains prodomain and metalloprotease domain--amino acids 1-459DepositorInserttruncated ADAM metallopeptidase domain 10 (ADAM10 Human)
UseTagsVFExpressionMammalianMutationcontains aa 1-459PromoterAvailable sinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only