Addgene

We narrowed to 3,077 results for: zebrafish

Showing: 281 - 300 of 3077 results

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pTol2-nbt300 2n-KalNFB-syt2a

Plasmid

#196901

Purpose

zebrafish expression

Depositor

Reinhard Köster

Insert

nbt300 2n-KalNFB-syt2a

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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Tol2-elavl3-GCaMP6s

Plasmid

#59531

Purpose

Expressed GCaMP6-slow, a calcium indicator, in neurons in zebrafish larva. The plasmid is in Tol2 transposon backbone

Depositor

Misha Ahrens

Article

Vladimirov et al Nat Methods. 2014 Jul 27. doi: 10.1038/n

Inserts

elavl3 promoter (elavl3 Zebrafish)
GCaMP6s

Use

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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MiniCoopR U6:gRNA p53, mitfa:Cas9

Plasmid

#118841

Purpose

Targets tp53 and expresses zebrafish mitfa specifically in zebrafish melanocytes

Depositor

Leonard Zon

Article

Ablain et al Science. 2018 Nov 1. pii: science.aau650

Insert

tp53 gRNA (tp53 Zebrafish)

Use

CRISPR; Tol2

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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MiniCoopR U6:gRNA spred1, mitfa:Cas9

Plasmid

#118843

Purpose

Targets spred1 and expresses zebrafish mitfa specifically in zebrafish melanocytes

Depositor

Leonard Zon

Article

Ablain et al Science. 2018 Nov 1. pii: science.aau650

Insert

spred1 gRNA (spred1 Zebrafish)

Use

CRISPR; Tol2

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pTol2pA2-ubb:secHsa.ANNEXINV-mVenus, cryaa:RFP

Plasmid

#92388

Purpose

Cell death reporter

Depositor

Nicholas Cole

Article

Morsch et al Front Cell Neurosci. 2015 Aug 31;9:321.

Insert

ANNEXINV (ANXA5 Human)

Use

Zebrafish expression

Tags

mVenus

Expression

Mutation

Promoter

ubb

Availability

Academic Institutions and Nonprofits only

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pTol2CG2-QUAS5x:GFPNLS-SV40pA

Plasmid

#155122

Purpose

Tol2 vector containing QUAS5x reporter element upstream of GFP-NLS. Includes cmlc2:mRFP-SV40pA transgenesis marker

Depositor

Bret Pearson

Article

Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

Insert

QUAS5x:GFPNLS

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

QUAS5x-E1b

Availability

Academic Institutions and Nonprofits only

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pTol2CG2-QUASM1:GFPNLS-SV40pA

Plasmid

#155123

Purpose

Tol2 vector containing QUASM1 reporter element upstream of GFP-NLS. Includes cmlc2:mRFP-SV40pA transgenesis marker

Depositor

Bret Pearson

Article

Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

Insert

QUASM1:GFPNLS

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

QUASM1-E1b

Availability

Academic Institutions and Nonprofits only

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pTol2CG2-QUASM1:mScarletNLS-SV40pA

Plasmid

#155124

Purpose

Tol2 vector containing QUASM1 reporter element upstream of mScarlet-NLS. Includes cmlc2:mRFP-SV40pA transgenesis marker

Depositor

Bret Pearson

Article

Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

Insert

QUASM1:mScarletNLS

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

QUASM1-E1b

Availability

Academic Institutions and Nonprofits only

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pTol2-ubi:loxP-mScarletNLS-SV40pA-loxp-QFGal4-SV40pA

Plasmid

#155125

Purpose

Tol2 vector containing Cre-inducible QFGal4-switch

Depositor

Bret Pearson

Article

Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

Insert

QFGal4-switch

Use

Cre/Lox; Zebrafish expression

Tags

Expression

Mutation

Promoter

Ubi

Availability

Academic Institutions and Nonprofits only

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Tol2-LyzC-RacFRET-U6ac-ctrl guides

Plasmid

#168247

Purpose

"label active Rac; control for neutrophil-specific knockout"

Depositor

Qing Deng

Article

Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

Insert

RacFRET

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

LyzC

Availability

Academic Institutions and Nonprofits only

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Tol2-LyzC-RacFRET-U6ac-rac2 guides

Plasmid

#168248

Purpose

"label active Rac under Rac2 neutrophil-specific knockout background"

Depositor

Qing Deng

Article

Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

Insert

RacFRET

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

LyzC

Availability

Academic Institutions and Nonprofits only

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Tol2-LyzC-PBD-GFP-U6ac-rac2 guides

Plasmid

#168251

Purpose

"label active Rac under Rac2 neutrophil-specific knockout background"

Depositor

Qing Deng

Article

Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

Insert

PBD GFP

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

LyzC

Availability

Academic Institutions and Nonprofits only

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Tol2-LyzC-PBD-GFP-U6ac-ctrl guides

Plasmid

#168252

Purpose

"label active Rac; control for neutrophil-specific knockout"

Depositor

Qing Deng

Article

Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

Insert

PBD GFP

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

LyzC

Availability

Academic Institutions and Nonprofits only

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Tol2-LyzC-Utrophin-GFP-U6ac-ctrl guides

Plasmid

#168253

Purpose

"label stable F-actin; control for neutrophil-specific knockout"

Depositor

Qing Deng

Article

Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

Insert

Utrophin-GFP

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

LyzC

Availability

Academic Institutions and Nonprofits only

Enlarge
Tol2-LyzC-Utrophin-GFP-U6ac-Rac2 guides

Plasmid

#168254

Purpose

"label stable F-actin under Rac2 neutrophil-specific knockout background"

Depositor

Qing Deng

Article

Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

Insert

Utrophin-GFP

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

LyzC

Availability

Academic Institutions and Nonprofits only

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pT3TS-spCas9 ABE-Umax-ex1-rest1

Plasmid

#222140

Purpose

Adenine base editor with modified TadA variant for extended window and restricted A-to-G base editing in zebrafish

Depositor

Gaurav Varshney

Article

Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

Insert

Modified TadA-nSpCas9

Use

CRISPR; Zebrafish expression

Tags

Bipartite NLS

Expression

Mutation

Promoter

T3

Availability

Academic Institutions and Nonprofits only

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pT3TS-spCas9 ABE-Umax-ex2-rest1

Plasmid

#222143

Purpose

Adenine base editor with modified TadA variant for extended window and restricted A-to-G base editing in zebrafish

Depositor

Gaurav Varshney

Article

Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

Insert

Modified TadA-nSpCas9

Use

CRISPR; Zebrafish expression

Tags

Bipartite NLS

Expression

Mutation

Promoter

T3

Availability

Academic Institutions and Nonprofits only

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ZP42 Vent-CS (ZV86)

Plasmid

#16900

Depositor

Randall Moon

Insert

zvox-1 (vent Zebrafish)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only
ZP10 Snail

Plasmid

#16931

Depositor

Randall Moon

Insert

snail (snai1a Zebrafish)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only
ZP9 Actin

Plasmid

#16932

Depositor

Randall Moon

Insert

Beta-actin (actb1 Zebrafish)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only