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Plasmid#122728PurposeExpress HPV16 E7 TCRDepositorInsertHPV16 E7 TCR
UseRetroviralTagsExpressionMutationPromoterAvailable sinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
nls-mCherry
Plasmid#108881PurposeExpression of nuclear localized mCherryDepositorInsertnls-mCherry
UseCan be used for mammalian expression or in vitro …TagsExpressionMammalianMutationPromoterAvailable sinceDec. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSB1C3-H3
Plasmid#109381PurposeArabinose inducible VioB-mCherry expression (weaker RBS). Used to test burden response in E. coliDepositorInsertpARABAD-VioB-mCherry
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
FLAG SMG1 WT
Plasmid#199573PurposeExpresses FLAG-tagged WT SMG1DepositorInsertSMG1 (SMG1 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDN34-BiNLS-hYAP1-1delta
Plasmid#169471Purposeexpresses optoYAP in mammalian cellsDepositorInsertYAP1-1delta (YAP1 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceOct. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBAD-R-GECO1.2
Plasmid#113937PurposeBacterial expression of red fluorescent Ca2+ sensor R-GECO1.2DepositorInsertR-GECO1.2
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLVX-IRES-tdTomato-FlagAkt1
Plasmid#64831PurposeLentiviral expression of murine Flag-Akt1DepositorInsertAkt1 (Akt1 Mouse)
UseLentiviralTags3xFlagExpressionMammalianMutationPromoterCMVAvailable sinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
LV-TRE-VP64 mouse MyoD-T2A-dsRedExpress2
Plasmid#60625PurposeExpresses VP64 mouse MyoD and dsRedExpress2 in response to doxycyclineDepositorInsertVP64 mouse MyoD
UseLentiviralTagsExpressionMutationPromoterTREAvailable sinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV2-GCaMP6f-P2A-H2B-PAmCherry
Plasmid#133419PurposeFluorescent reporter for calcium imaging and photoactivationDepositorInsertGCaMP6f-P2A-H2B-PAmCherry (H2BC11 Human)
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceDec. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOZ-N-FH RAP80
Plasmid#27501DepositorInsertRAP80 (UIMC1 Human)
UseRetroviralTagsFLAG and HAExpressionMammalianMutationPromoterAvailable sinceMarch 1, 2011AvailabilityAcademic Institutions and Nonprofits only -
H2B-mCherry
Plasmid#108880PurposeExpression of human histone2B fused to mCherry (nuclear marker)DepositorInsertH2B-mCherry
UseCan be used for mammalian expression or in vitro …TagsExpressionMammalianMutationPromoterAvailable sinceJune 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
mKate2-P2A-APEX2-csGBP
Plasmid#108875PurposeExpression of APEX2 fused to a conditionally stable nanobody against GFP (includes red cytoplasmic reporter)DepositorInsertmKate2-P2A-APEX2-csGBP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
1457_pDEST_miniTol2_R4-R2_Cryst_mCherry
Plasmid#171796PurposepDEST miniTol2-clone for R4-R2 3-component gateway assembly (p5E + pME). Include a mCherry selection marker (Crystallin promoter Lens/Eyes)DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-hygro-eGFP-HEC1-9A
Plasmid#114028PurposeInducible expression of eGFP-tagged HEC1-9A in FLPin Trex cell linesDepositorInserteGFP-HEC1 - 9A (NDC80) (NDC80 Human)
UseTagsExpressionMammalianMutationHec1 - 9APromoterAvailable sinceSept. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBAD-O-GECO1
Plasmid#113938PurposeBacterial expression of orange fluorescent Ca2+ sensor O-GECO1DepositorInsertO-GECO1
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLV-CMV-tagRFP-Mad2
Plasmid#114021PurposeLentiviral delivery of tagRFP-MAD2 expressed from the CMV promoterDepositorInserttagRFP-Mad2 (MAD2L1) (MAD2L1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSB1C3-Lux
Plasmid#109383PurposeArabinose inducible expression of the Vibrio Fischery Lux operon. Used to test burden response in E. coliDepositorInsertpARABAD-LUX operon
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pME-secAnxaV-NS
Plasmid#67718PurposeGateway entry clone containing secreted human AnxaV with no stop codonDepositorInsertsecAnxaV-NS
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
LV-TRE-VP16 mouse MyoD-T2A-dsRedExpress2
Plasmid#60626PurposeExpresses VP16 mouse MyoD and dsRedExpress2 in response to doxycyclineDepositorInsertVP16 mouse MyoD
UseLentiviralTagsExpressionMutationPromoterTREAvailable sinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCSN075
Plasmid#166732PurposeSingle copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to NLS and Mxi, codon optimized for Saccharomyces cerevisiaeDepositorInsertdCas12a-NLS-Mxi1
UseCRISPRTagsMxi1 and SV40 NLSExpressionYeastMutationE925APromoterS. cerevisiae TEF1Available sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only