Addgene

We narrowed to 818 results for: nanobody

Showing: 281 - 300 of 818 results

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pCDNA3.0_nucLAMA-F98 IRES mYFP

Plasmid

#130712

Purpose

Plasmid encoding nuclear localization of LAMA-F98 for GFP as a SNAP-Tag-fusion and mYFP

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

SNAP-Tag-LAMA-F98-NLS IRES mYFP

Use

Tags

NLS and SNAP-Tag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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LSS-SGFP2_H2A

Plasmid

#112937

Purpose

Visualization of histones

Depositor

Dorus Gadella

Article

Mahlandt et al bioRxiv 2022.11.24.517828

Insert

H2A

Use

Tags

LSS-SGFP2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA216-CoV2-RBD-gamma variant

Plasmid

#194600

Purpose

Mammalian expression of RBD domain from SARS-CoV-2 gamma

Depositor

Ben Murrell

Insert

CoV2-RBD-gamma variant (S Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA216-CoV2-RBD-delta variant

Plasmid

#194601

Purpose

Mammalian expression of RBD domain from SARS-CoV-2 delta

Depositor

Ben Murrell

Insert

CoV2-RBD-delta variant (S Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA216-CoV2-RBD-alpha variant

Plasmid

#194599

Purpose

Mammalian expression of RBD domain from SARS-CoV-2 alpha

Depositor

Ben Murrell

Insert

CoV2-RBD-alpha variant (S Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 10, 2023

Availability

Academic Institutions and Nonprofits only

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iRFP670 - H2A

Plasmid

#176092

Purpose

nuclear marker

Depositor

Dorus Gadella

Article

Mahlandt et al bioRxiv 2022.11.24.517828

Insert

H2A

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA3.0_mitoLAMA-F98 IRES ShadowG-mScarlet

Plasmid

#130708

Purpose

Plasmid encoding outer mitochondrial localization of LAMA-F98 for GFP as a SNAP-Tag-fusion and ShadowG-mScarlet

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

NTOM20-SNAP-Tag-LAMA-F98 IRES ShadowG-mScarlet

Use

Tags

NTOM20 and SNAP-Tag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA3.0_LynLAMA-G97 IRES EGFP

Plasmid

#130711

Purpose

Plasmid encoding outer inner membrane localization of LAMA-G97 for GFP as a SNAP-Tag-fusion and EGFP

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

Lyn11-SNAP-Tag-LAMA-G97 IRES EGFP

Use

Tags

Lyn11 and SNAP-Tag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA3.0_LynLAMA-F98 IRES EGFP

Plasmid

#130710

Purpose

Plasmid encoding outer inner membrane localization of LAMA-F98 for GFP as a SNAP-Tag-fusion and EGFP

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

Lyn11-SNAP-Tag-LAMA-F98 IRES EGFP

Use

Tags

Lyn11 and SNAP-Tag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA3.0_mitoLAMA-F98 IRES EGFP

Plasmid

#130706

Purpose

Plasmid encoding outer mitochondrial localization of LAMA-F98 for GFP as a SNAP-Tag-fusion and EGFP

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

NTOM20-SNAP-Tag-LAMA-F98 IRES EGFP

Use

Tags

NTOM20 and SNAP-Tag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3.0_mitoLAMA-G97 IRES ShadowG-mScarlet

Plasmid

#130709

Purpose

Plasmid encoding outer mitochondrial localization of LAMA-G97 for GFP as a SNAP-Tag-fusion and ShadowG-mScarlet

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

NTOM20-SNAP-Tag-LAMA-G97 IRES ShadowG-mScarlet

Use

Tags

NTOM20 and SNAP-Tag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3.0_mitoLAMA-G97 IRES EGFP

Plasmid

#130707

Purpose

Plasmid encoding outer mitochondrial localization of LAMA-G97 for GFP as a SNAP-Tag-fusion and EGFP

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

NTOM20-SNAP-Tag-LAMA-G97 IRES EGFP

Use

Tags

NTOM20 and SNAP-Tag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-30

Plasmid

#172754

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-30; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-30

Use

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaM-4

Plasmid

#172770

Purpose

Bacterial expression of anti-mCherry nanobody LaM-4, with pelB leader and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaM-4

Use

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-16-4GS-LaG-2

Plasmid

#172764

Purpose

Bacterial expression of dimerized anti-GFP nanobodies LaG-16 and LaG-2 (4GS linker)

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-16-4GS-LaG-2

Use

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-16

Plasmid

#172746

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-16; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-16

Use

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-pelB-LaM-3

Plasmid

#172769

Purpose

Bacterial expression of anti-mCherry nanobody LaM-3, with pelB leader and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaM-3

Use

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-pelB-LaM-8

Plasmid

#172772

Purpose

Bacterial expression of anti-mCherry nanobody LaM-8, with pelB leader and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaM-8

Use

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-pelB-LaG-41-4GS-LaG-2

Plasmid

#172766

Purpose

Bacterial expression of dimerized anti-GFP nanobodies LaG-41 and LaG-2 (4GS linker)

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-41-4GS-LaG-2

Use

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-pelB-LaG-10

Plasmid

#172743

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-10; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-10

Use

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 281 - 300 of 818 results

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