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Plasmid#217796PurposeVariant CE1 construct with Cdc13 telomere binding protein for template recruitment, expressed from CMV or T7 promoters.DepositorInsertCdc13-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationnSpCas9(H840A); EcKlenow(-exo;D355A/E357A)PromoterCMV and T7Available sinceSept. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEF1a_HH-EMX1 spacer-gRNA scaffold-EC73-5'-donor template-EC73-3'-HDV_pA_pCMV_EGFP-L138S-P2A-mCherry_pA
Plasmid#176459PurposeVector for expressing retron Ec73ncRNA-gRNA chimeric RNA (rgRNA) targeting EMX1 driven by EF1alpha promoter and and EGFP(L138S)-P2A-mCherry driven by CMVDepositorInsertHH-EMX1 spacer-gRNA scaffold-Ec73ncRNA-donor sequence-HDV
UseCRISPRTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEF1a_HH-HEK3 spacer-gRNA scaffold-EC73-5'-donor template-EC73-3'-HDV_pA_pCMV_EGFP-L138S-P2A-mCherry_pA
Plasmid#176460PurposeVector for expressing retron Ec73ncRNA-gRNA chimeric RNA (rgRNA) targeting HEK3 site driven by EF1alpha promoter and EGFP(L138S)-P2A-mCherry driven by CMVDepositorInsertHH-HEK3 spacer-gRNA scaffold-Ec73ncRNA-donor sequence-HDV
UseCRISPRTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
Click editor utilizing Cdc13(Y556A) for template recruitment - pCMV-T7-Cdc13(Y556A)-nCas9-EcKlenow (JO1329)
Plasmid#217797PurposeVariant CE1 construct with Cdc13(Y556A) telomere binding protein for template recruitment, expressed from CMV or T7 promoters.DepositorInsertCdc13(Y556A)-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationCdc13(Y556A); nSpCas9(H840A); EcKlenow(-exo;D355A…PromoterCMV and T7Available sinceSept. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
Click editor utilizing mSA for template recruitment and ePhi29 DNA polymerase - pCMV-T7-mSA-nCas9-ePhi29 (JO1398)
Plasmid#217803PurposeVariant CE1 construct with mSA for template recruitment and ePhi29(D169A) DNA pol, expressed from CMV or T7 promoters.DepositorInsertmSA-linker-SV40NLS-nSpCas9-BPNLS-ePhi29-BPNLS
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationnSpCas9(H840A); Phi29(D169A/M8R/V51A/M97T/G197D/E…PromoterCMV and T7Available sinceSept. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC7A6
Plasmid#132209PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC7A6 (SLC7A6 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 28, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCDNA3.1neo-NLucYAP15
Plasmid#107610Purposemeasure LATS activity by Luciferase AssayDepositorInsertNLucYAP15
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-SLC7A6_STOP
Plasmid#161375PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.DepositorInsertSLC7A6 (SLC7A6 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 15, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCDNA3.1neo-14-3-3-CLuc
Plasmid#107611Purposemeasure LATS activity by Luciferase AssayDepositorInsertpCDNA3.1hygro-BamHI-CLuc1433-NotI
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
p1xFlag CMV2 WW45
Plasmid#18970DepositorInsertSALVADOR 1 (SAV1 Human)
UseTags1xFlagExpressionMammalianMutationPromoterAvailable sinceSept. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
p2xFlag CMV2-YAP2
Plasmid#19045DepositorInsertYes-kinase associated protein (YAP1 Human)
UseTags2xFlagExpressionMammalianMutationPromoterAvailable sinceSept. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/HisMaxB-YAP1
Plasmid#18978DepositorInsertYes-kinase associated protein (YAP1 Human)
UseTagsHisExpressionMammalianMutationPromoterAvailable sinceSept. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
pEGFP C3-Mst2
Plasmid#19056DepositorInsertMammalian Ste20-like kinase 2 (STK3 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceSept. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
pEGFP C1-WW45
Plasmid#19052DepositorInsertSALVADOR 1 (SAV1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceSept. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2TK-YAP-Ag
Plasmid#19063DepositorInsertYes-kinase associated protein (YAP1 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceSept. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
pDsRed Monomer C1-YAP2
Plasmid#19057DepositorInsertYes-kinase associated protein (YAP1 Human)
UseTagsRed-FPPExpressionMammalianMutationPromoterAvailable sinceSept. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
pEGFP C3-YAP2
Plasmid#19055DepositorInsertYes-kinase associated protein (YAP1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceSept. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
p2xFlag CMV2-YAP2-S127D mutant
Plasmid#19051DepositorInsertYes-kinase associated protein (YAP1 Human)
UseTags2xFlagExpressionMammalianMutationSerine 127 changed to Aspartic AcidPromoterAvailable sinceSept. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
pHR Lck-TagBFP
Plasmid#171034PurposeTagBFP-tagged Lck kinaseDepositorInsertLck
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
p2xFlag CMV2-YAP2-S127A mutant
Plasmid#19050DepositorInsertYes-kinase associated protein (YAP1 Human)
UseTags2xFlagExpressionMammalianMutationSerine 127 changed to AlaninePromoterAvailable sinceSept. 8, 2008AvailabilityAcademic Institutions and Nonprofits only