Addgene

We narrowed to 1,780 results for: ipp

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pCIG-LRRC37B

Plasmid

#220790

Purpose

To express the human receptor LRRC37B in mammalian cells together with EGFP

Depositor

Pierre Vanderhaeghen

Article

Libe-Philippot et al Cell. 2023 Dec 21;186(26):5766-5783.e25.

Insert

LRRC37B (LRRC37B Human)

Use

Tags

Expression

Mutation

Promoter

Available since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pBS-KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XOllas

Plasmid

#217512

Purpose

KD Recombinase dependent conditional smGdP-10XOllas cassette

Depositor

S. Lawrence Zipursky

Article

Sanfilippo et al Neuron. 2024 Jan 13:S0896-6273(23)00973-

Insert

KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XOllas

Use

Tags

smGdP-10XOllas

Expression

Insect

Mutation

Promoter

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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PHR-GFP-VP16

Plasmid

#183927

Purpose

Optogenetic PHR domain coupled to GFP and VP16 activation domain; binds to CIBN upon blue light exposure

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

PHR-GFP-VP16 (CRY2, UL48 Synthetic, Mustard Weed)

Use

Tags

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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mEmerald-GPAT4152-208

Plasmid

#134468

Purpose

GPAT4152-208 expression

Depositor

Jennifer Lippincott-Schwartz

Article

Chang et al J Cell Biol. 2019 Aug 5;218(8):2583-2599

Insert

GPAT4 (GPAT4 Human)

Use

Tags

Expression

Mammalian

Mutation

GPAT4, amino acids 152-208

Promoter

CMV

Available since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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Tol2 8xSTAR-mNeonGreen. PGK-puro

Plasmid

#136255

Purpose

Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

Depositor

Hugo Snippert

Article

Oost et al Cell Rep. 2018 Feb 6;22(6):1600-1614. do

Insert

stem cell Ascl2 reporter, 8 repeats

Use

Tol2 transposase

Tags

mNeonGreen

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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TVBB C-term-mScarlet-Blast

Plasmid

#169221

Purpose

Targeting vector backbone to support a knock-in of Linker-mScarlet-P2A-Blast at the C-terminus of a target locus

Depositor

Hugo Snippert

Article

Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

Insert

Double SapI flanked mScarlet-P2A-Blast

Use

Targeting vector backbone

Tags

Expression

Mutation

Promoter

Available since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pLV 4xSTAR-mTagBFP2-blast

Plasmid

#125239

Purpose

Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

Depositor

Hugo Snippert

Article

Oost et al Cell Rep. 2018 Feb 6;22(6):1600-1614. do

Insert

stem cell Ascl2 reporter, 4 repeats

Use

Lentiviral

Tags

mTagBFP2 (codon optimized)

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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TVBB C-term-mScarlet-Puro

Plasmid

#169220

Purpose

Targeting vector backbone to support a knock-in of Linker-mScarlet-P2A-Puro at the C-terminus of a target locus

Depositor

Hugo Snippert

Article

Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

Insert

Double SapI flanked mScarlet-P2A-Puro

Use

Targeting vector backbone

Tags

Expression

Mutation

Promoter

Available since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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TVBB C-term-mTurquoise2-Hygro

Plasmid

#169225

Purpose

Targeting vector backbone to support a knock-in of Linker-mTurquoise2-P2A-Hygro at the C-terminus of a target locus

Depositor

Hugo Snippert

Article

Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

Insert

Double SapI flanked mTurquoise2-P2A-Hygro

Use

Targeting vector backbone

Tags

Expression

Mutation

Promoter

Available since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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Halo-p58

Plasmid

#166896

Purpose

mammalian expression of p58 (ie ERGIC-53; LMAN1) tagged with HaloTag

Depositor

Jennifer Lippincott-Schwartz

Article

Weigel et al Cell. 2021 Apr 29;184(9):2412-2429.e16.

Insert

p58 (Lman1 Rat)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

L405Q, R419S- please see depositor comments

Promoter

CMV

Available since

May 13, 2021

Availability

Academic Institutions and Nonprofits only

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TVBB C-term-mNeongreen-Puro

Plasmid

#169228

Purpose

Targeting vector backbone to support a knock-in of Linker-mNeongreen-P2A-Puro at the C-terminus of a target locus

Depositor

Hugo Snippert

Article

Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

Insert

Double SapI flanked mNeongreen-P2A-Puro

Use

Targeting vector backbone

Tags

Expression

Mutation

Promoter

Available since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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TVBB C-term-mTurquoise2-Puro

Plasmid

#169224

Purpose

Targeting vector backbone to support a knock-in of Linker-mTurquoise2-P2A-Puro at the C-terminus of a target locus

Depositor

Hugo Snippert

Article

Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

Insert

Double SapI flanked mTurquoise2-P2A-Puro

Use

Targeting vector backbone

Tags

Expression

Mutation

Promoter

Available since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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TVBB C-term-mNeongreen-Blast

Plasmid

#169229

Purpose

Targeting vector backbone to support a knock-in of Linker-mNeongreen-P2A-Blast at the C-terminus of a target locus

Depositor

Hugo Snippert

Article

Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

Insert

Double SapI flanked mNeongreen-P2A-Blast

Use

Targeting vector backbone

Tags

Expression

Mutation

Promoter

Available since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pCIBN-hTRF2-tagRFP-T

Plasmid

#103812

Purpose

BLInCR 'Localizer' construct that marks telomeres and is targeted by a PHR-tagged effector upon illumination with blue light; can be visualized without triggering PHR recruitment

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

CIBN-hTRF2-tagRFP-T (CIB1, TERF2 Human, Mustard Weed)

Use

Tags

Expression

Mammalian

Mutation

hTRF2: deletion of amino acids 1-42 and 476 compa…

Promoter

CMV

Available since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_VNp-LZ_VNp-LZ-mNeongreen-Etanercept

Plasmid

#182424

Purpose

Bacterial expression of Vesicle Nucleating peptide-Leucine Zipper and Vesicle Nucleating peptide-Leucine Zipper-mNeongreen-Etanercept fusion proteins

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Inserts

VNp-LZ-mNeongreen-Etanercept (TNFRSF1B Human)
VNp-LZ

Use

Tags

Vesicle Nucleating peptide (VNp), and Vesicle Nuc…

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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PHR-GFP-FUSN-VP16

Plasmid

#183933

Purpose

Optogenetic PHR domain coupled to GFP, FUSN (high phase separation propensity) and VP16 activation domain; binds to CIBN upon blue light exposure

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

PHR-GFP-FUSN-VP16 (CRY2, UL48 Synthetic, Mustard Weed)

Use

Tags

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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pCIBN-tagBFP-hNCL

Plasmid

#103805

Purpose

BLInCR 'Localizer' construct that marks the nucleoli and is targeted by a PHR-tagged effector upon illumination with blue light

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

CIBN-tagBFP-hNCL (CIB1, NCL Human, Mustard Weed)

Use

Tags

Expression

Mammalian

Mutation

hNCL: A395G (K132R), A421G (K141E), A424G (K142E)…

Promoter

CMV

Available since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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TVBB C-term-Dendra2-Puro

Plasmid

#169217

Purpose

Targeting vector backbone to support a knock-in of Linker-Dendra2-P2A-Puro at the C-terminus of a target locus

Depositor

Hugo Snippert

Article

Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

Insert

Double SapI flanked Dendra2-P2A-Puro

Use

Targeting vector backbone

Tags

Expression

Mutation

Promoter

Available since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pCIBN-LacI

Plasmid

#103814

Purpose

BLInCR 'Localizer' construct that marks lacO arrays and is targeted by a PHR-tagged effector upon illumination with blue light

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

CIBN-LacI (CIB1, lacI E. coli, Mustard Weed)

Use

Tags

Expression

Mammalian

Mutation

LacI: C19T (silent, L7L), T264C (silent, A88A), C…

Promoter

CMV

Available since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pCIBN-EYFP-hNCL

Plasmid

#103806

Purpose

BLInCR 'Localizer' construct that marks the nucleoli and is targeted by a PHR-tagged effector upon illumination with blue light

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

CIBN-EYFP-hNCL (CIB1, NCL Human, Mustard Weed)

Use

Tags

Expression

Mammalian

Mutation

hNCL: A395G (K132R), A421G (K141E), A424G (K142E)…

Promoter

CMV

Available since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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