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Showing: 281 - 300 of 37829 results

  6. pTarget TMEM97-P2A-mCardinal

    Plasmid
    #157747
    Purpose
    Express in mammalian cells TMEM97 and mCardianl to follow transfection efficiency using flow cytometry. mCardinal is expressed following a P2A skip peptide so it is not connected to TMEM97.
    Depositor
    Andrew Kruse
    Article
    Alon et al Proc Natl Acad Sci U S A. 2017 Jul 3;114
    Insert
    TMEM97 (TMEM97 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 10, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    157747-plasmid-map-sequence-id-308120

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  8. pRW004-SM-destination-proUBQ10-Cas9

    Plasmid
    #104438
    Purpose
    Destination vector expressing plant-codon-optimized Cas9 under UBQ10 promoter, with sgRNAs be shuffled in; seed coat specific red fluorescence for screening trangene free plants;
    Depositor
    Detlef Weigel
    Article
    Wu et al Plant Methods. 2018 Aug 2;14:65. doi: 10
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Bacterial and Plant
    Mutation
    Promoter
    UBQ10
    Available since
    Jan. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    104438-plasmid-map-sequence-id-201144

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  18. pU6_(GLuc)_INT[Spinach2]

    Plasmid
    #68430
    Purpose
    Transient expression in mammalian cells of an "INT" construct_bearing the "Spinach2" aptamer, targeting the GLuc reporter. U6 promoter
    Depositor
    John Rinn
    Article
    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:
    Insert
    INT construct_bearing the "Spinach2" aptamer
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    human U6
    Available since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    68430-plasmid-map-sequence-id-120208

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  19. pGL3-noSV40-humanEC1.45_min1-2_1XCoordinatorMutant

    Plasmid
    #173963
    Purpose
    Firefly luciferase enhancer reporter plasmid.
    Depositor
    Joanna Wysocka
    Article
    Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783
    Insert
    Minimal enhancer regions 1 and 2 (min1 and min2) from SOX9 enhancer cluster EC1.45 with 1 mutated Coordinator motif
    Use
    Luciferase
    Tags
    Expression
    Mutation
    1 mutated Coordinator motif
    Promoter
    SV40 promoter
    Available since
    Sept. 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    173963-plasmid-map-sequence-id-341768

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Showing: 281 - 300 of 37829 results