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Plasmid#120275PurposeBlunt-end cloning vector of PCR-products using ZERO-backgound systemDepositorTypeEmpty backboneUseTagsExpressionMutationPromoterAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only
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pET15-MHL
Plasmid#26092PurposeSGC Empty backbone for bacterial expression under T7 promoter. Uses infusion based cloning method.DepositorTypeEmpty backboneUseTagsHis and TEV cleavage siteExpressionBacterialMutationPromoterT7-lacOAvailable sinceSept. 6, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
p15TV-L
Plasmid#26093PurposeSGC Empty backbone for bacterial expression under T7 promoter. Uses infusion based cloning method.DepositorTypeEmpty backboneUseTags6xHis and TEV cleavage siteExpressionBacterialMutationPromoterT7-lacOAvailable sinceSept. 6, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pME-myc-BirA (JDW 1210)
Plasmid#224509PurposeGateway compatible middle entry clone containing a Myc tagged BirADepositorInsertMyc-BirA
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME-TAEL (JDW 1324)
Plasmid#224508PurposeGateway compatible middle entry clone containing TAEL2.0 transcription factor for making binary TAEL system vectors (generating a tissue specific driver)DepositorInsertTAEL
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME-Myc-BioID2-MCS1 (JDW 1095)
Plasmid#224510PurposeGateway compatible middle entry clone containing a Myc-tagged BIOID2 constructDepositorInsertMyc-BioID2
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME-Myc-BioID2-MCS2 (JDW 1209)
Plasmid#224511PurposeA Gateway compatible middle clone with a Myc-tagged BIOID2 construct followed by a flexible linker and an MCSDepositorInsertMyc-BioID2
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME-3x-FLAG-Ubiquitin (JDW 1098)
Plasmid#224513PurposeA Gateway compatible middle entry clone containing a 3x FLAG tagged human Ubiquitin for monitoring ubiquitylation statusDepositorInsert3x-FLAG-Ubiquitin
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME-DreERT2 (JDW 1344)
Plasmid#224523PurposeA Gateway compatible middle entry clone containing Dre recombinase fused to mutant ERT2 for tamoxifen-inducible recombinase activityDepositorInsertDreERT2
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME EFS-rtTA/rtTA3 (JDW 1230)
Plasmid#224525PurposeA Gateway compatible middle entry clone containing a rtTA-3rd generation transactivatorDepositorInsertEFS-rtTA
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
p3E-V5-mClover3-pA (JDW 1318)
Plasmid#224530PurposeA Gateway compatible 3' entry clone containing a V5 tagged mClover3 followed by a polyADepositorInsertV5-mClover3-pA
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
p3E-IRES-mTagBFP2-pA (JDW 1361)
Plasmid#224535PurposeA Gateway compatible 3' entry clone containing an IRES-FLAG-NLS-mTagBFP2-pADepositorInsertIRES-FLAG-NLS-mTagBFP2-pA
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-Lifeact- mScarlet-HA (JDW 1247)
Plasmid#224538PurposeA Gateway compatible middle entry clone containing Lifeact-mScarlet-I (far red f-actin reporter)DepositorInsertLifeact-mScarlet-HA
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pelBHisTEV
Plasmid#157741Purposeempty vector; TEV-protease-removable N-term PelB signal sequence +His10; potential C-term His6; T7lac promoter; Kan-R; avoids added residues in final protein since cloning uses BseRI restriction sitesDepositorTypeEmpty backboneUseTagsPelB signal sequence + His10-tag + TEV protease c…ExpressionBacterialMutationPromoterT7lacAvailable sinceJune 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pT7-pelB
Plasmid#157740Purposeempty vector; N-terminal PelB signal sequence; potential C-terminal His6; T7 promoter; like pET-20b, but additionally avoids added residues in final protein since cloning uses BseRI restriction sitesDepositorTypeEmpty backboneUseTagsPelB signal sequence and possible His6-tag (adds …ExpressionBacterialMutationPromoterT7Available sinceJune 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pICE ADE2
Plasmid#122876PurposeADE2 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.DepositorTypeEmpty backboneUseYeast recombination cloningTagsExpressionYeastMutationPromoterAvailable sinceMay 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pICE HIS3
Plasmid#122877PurposeHIS3 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.DepositorTypeEmpty backboneUseYeast recombination cloningTagsExpressionYeastMutationPromoterAvailable sinceFeb. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pICE TRP1
Plasmid#122881PurposeTRP1 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.DepositorTypeEmpty backboneUseYeast recombination cloningTagsExpressionYeastMutationPromoterAvailable sinceOct. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pICE LEU2
Plasmid#122878PurposeLEU2 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.DepositorTypeEmpty backboneUseYeast recombination cloningTagsExpressionYeastMutationPromoterAvailable sinceDec. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pICE LYS2
Plasmid#122879PurposeLYS2 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.DepositorTypeEmpty backboneUseYeast recombination cloningTagsExpressionYeastMutationPromoterAvailable sinceMay 25, 2019AvailabilityAcademic Institutions and Nonprofits only