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Plasmid#67662PurposeMammalian expression vector driving APEX2-TAPP1 (Human TAPP1-PH domain, [marks PI(3,4)P2] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.DepositorInsertmKate2-P2A-APEX2-TAPP1-PH
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
mKate2-P2A-APEX2-GBP
Plasmid#67650PurposeMammalian expression vector driving APEX2-GBP (EM peroxidase marker fused to GFP-targeting nanobody). Also contains bicistronially expressed cytoplasmic red fluorescent reporter.DepositorInsertAPEX2-GBP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
mKate2-P2A-APEX2-ML1N*2
Plasmid#67665PurposeMammalian expression vector driving APEX2-ML1N (Mouse 2xML1N domain, [marks PI(3,5)P2] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.DepositorInsertmKate2-P2A-APEX2-ML1N*2
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
AKT-APEX2-P2A-mKate2
Plasmid#67654PurposeMammalian expression vector driving AKT-APEX2 (Human AKT-PH domain, [marks PI(3,4)P2 and PI(3,4,5)P3] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.DepositorInsertAKT-PH-APEX2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
BTK-APEX2-P2A-mKate2
Plasmid#67653PurposeMammalian expression vector driving BTK-APEX2 (Human BTK-PH domain, [marks PI(3,4,5)P3] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red fluorescent reporter.DepositorInsertBTK-PH-APEX2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
2PH-PLCd-APEX2-P2A-mKate2
Plasmid#71284PurposeMammalian expression vector driving 2PH-PLC-delta-APEX2 (Human 2xPLCd PH domain, [marks PI(4,5)P2] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporterDepositorInsert2PH-PLCd-APEX2-P2A-mKate2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
mKate2-P2A-APEX2-2xFYVE_hrs
Plasmid#67663PurposeMammalian expression vector driving APEX2-2xFYVE (2xMouse FYVE domain from HRS, [marks PI3P] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.DepositorInsertmKate2-P2A-APEX2-2xFYVE
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
mKate2-P2A-APEX2-2xML1N(7Q)
Plasmid#67666PurposeMammalian expression vector driving APEX2-ML1N (Mouse 2xML1N domain (mutant version), [marks PI(3,5)P2] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.DepositorInsertmKate2-P2A-APEX2-ML1Nmut*2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
8024 GFP_Kras_G12C_2A_puro
Plasmid#64373PurposeThis a retroviral expression plasmid expressing GFP tagged G12C mutant Kras 2A oncogene along with puro resistance geneDepositorInsertmouse Kras2A (Kras Mouse)
UseRetroviralTagsGFPExpressionMutationchanged glycine 12 to cysteinePromoterAvailable sinceJuly 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
8027_GFP-KrasG12C_2B_puro
Plasmid#64372PurposeThis a retroviral expression plasmid expressing GFP tagged G12C mutant Kras 2B oncogene along with puro resistance geneDepositorInsertmouse Kras2B (Kras Mouse)
UseRetroviralTagsGFPExpressionMutationchanged glycine 12 to cysteinePromoterAvailable sinceJuly 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
8047_GFP-Kras2B_ires_puro
Plasmid#64371PurposeThis a retroviral expression plasmid expressing GFP tagged wild type Kras 2B oncogene along with puro resistance geneDepositorInsertmouse Kras2B (Kras Mouse)
UseRetroviralTagsGFPExpressionMutationnonePromoterAvailable sinceJuly 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
8798 GFP-KrasG12C_2B_hygro
Plasmid#64376PurposeThis a retroviral expression plasmid expressing GFP tagged G12C mutant Kras 2B oncogene along with hygro resistance geneDepositorInsertmouse Kras2B (Kras Mouse)
UseRetroviralTagsGFPExpressionMutationchanged glycine 12 to cysteinePromoterAvailable sinceJuly 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-jGCaMP8s-P2A-ChrimsonR-ST (AAV9)
Viral Prep#174007-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-hSyn-DIO-jGCaMP8s-P2A-ChrimsonR-ST (#174007). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-jGCaMP8s-P2A-ChrimsonR-ST plasmid DNA. Syn-driven, Cre-dependent expression of calcium sensor GCaMP8s and soma-targeted ChrimsonR. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsNoneAvailable sinceMay 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV-hSyn1-GCaMP6f-P2A-nls-dTomato (AAV1)
Viral Prep#51085-AAV1PurposeReady-to-use AAV1 particles produced from AAV-hSyn1-GCaMP6f-P2A-nls-dTomato (#51085). In addition to the viral particles, you will also receive purified AAV-hSyn1-GCaMP6f-P2A-nls-dTomato plasmid DNA. GCaMP6f calcium sensor and bicistronic, physically separate nuclear localized dTomato expression under the Synapsin promoter. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsnuclear dTomato (physically separate, not a fusion protein)Available sinceSept. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
AAV-hSyn1-GCaMP6s-P2A-nls-dTomato (AAV1)
Viral Prep#51084-AAV1PurposeReady-to-use AAV1 particles produced from AAV-hSyn1-GCaMP6s-P2A-nls-dTomato (#51084). In addition to the viral particles, you will also receive purified AAV-hSyn1-GCaMP6s-P2A-nls-dTomato plasmid DNA. GCaMP6s calcium sensor and bicistronic, physically separate nuclear localized dTomato expression under a human synapsin1 promoter. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsnuclear dTomato (physically separate, not a fusion protein)Available sinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-HA-hM4D(Gi)-IRES-mCitrine (AAV2)
Viral Prep#50467-AAV2PurposeReady-to-use AAV2 particles produced from pAAV-CaMKIIa-HA-hM4D(Gi)-IRES-mCitrine (#50467). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-HA-hM4D(Gi)-IRES-mCitrine plasmid DNA. CaMKIIa-driven HA-tagged hM4D(Gi) and bicistronic mCitrine expression, for chemogenetic inhibition. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCaMKIITagsmCitrineAvailable sinceMay 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAV-EF1a-DIO-GCaMP6s-P2A-nls-dTomato (AAV1)
Viral Prep#51082-AAV1PurposeReady-to-use AAV1 particles produced from AAV-EF1a-DIO-GCaMP6s-P2A-nls-dTomato (#51082). In addition to the viral particles, you will also receive purified AAV-EF1a-DIO-GCaMP6s-P2A-nls-dTomato plasmid DNA. GCaMP6s calcium sensor and bicistronic, physically separate nuclear localized dTomato expression under the EF1a promoter. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsnuclear dTomato (physically separate, not a fusion protein)Available sinceSept. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-HA-hM4D(Gi)-IRES-mCitrine (AAV8)
Viral Prep#50467-AAV8PurposeReady-to-use AAV8 particles produced from pAAV-CaMKIIa-HA-hM4D(Gi)-IRES-mCitrine (#50467). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-HA-hM4D(Gi)-IRES-mCitrine plasmid DNA. CaMKIIa-driven HA-tagged hM4D(Gi) and bicistronic mCitrine expression, for chemogenetic inhibition. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCaMKIITagsmCitrineAvailable sinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-GFAP-HA-rM3D(Gs)-IRES-mCitrine (AAV5)
Viral Prep#50472-AAV5PurposeReady-to-use AAV5 particles produced from pAAV-GFAP-HA-rM3D(Gs)-IRES-mCitrine (#50472). In addition to the viral particles, you will also receive purified pAAV-GFAP-HA-rM3D(Gs)-IRES-mCitrine plasmid DNA. GFAP-driven HA-tagged rM3D(Gs) and bicistronic mCitrine expression, for chemogenetic excitation. These AAV preparations are suitable purity for injection into animals.DepositorPromoterGFAPTagsmCitrineAvailable sinceJan. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV-hSyn1-GCaMP6f-P2A-nls-dTomato (AAV Retrograde)
Viral Prep#51085-AAVrgPurposeReady-to-use AAV Retrograde particles produced from AAV-hSyn1-GCaMP6f-P2A-nls-dTomato (#51085). In addition to the viral particles, you will also receive purified AAV-hSyn1-GCaMP6f-P2A-nls-dTomato plasmid DNA. GCaMP6f calcium sensor and bicistronic, physically separate nuclear localized dTomato expression under a human synapsin1 promoter. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsnuclear dTomato (physically separate, not a fusion protein)Available sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only