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Plasmid#1315DepositorInsertHsp104 L892W (HSP104 Budding Yeast)
UseTagsExpressionYeastMutationChanged Leu 892 to TrpPromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
UbG76V-eGFP-V5His_20aa
Plasmid#23970DepositorInsertUb-G76V-eGFP-V5HIS_20aa
UseTagseGFPExpressionMammalianMutationG76V mutation in Ubiquitin. Ub fused to N-termin…PromoterAvailable sinceMarch 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pPROEX Hsp104 (1-575)
Plasmid#1318DepositorInsertHsp104 (1-575) (HSP104 Budding Yeast)
UseTags6X His + TEV cleavageExpressionBacterialMutationaa1-575 of Hsp104PromoterAvailable sinceJan. 21, 2005AvailabilityAcademic Institutions and Nonprofits only -
pET151-Vps4(101-437)
Plasmid#87735PurposeBacterial expression of N-terminally truncated S. cerevisiae Vps4DepositorInsertVacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
UseTags6xHis-V5-TEVExpressionBacterialMutationdeleted amino acids 1-100PromoterT7Available sinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJC45BS104 Y819W
Plasmid#1307DepositorInsertHsp104 Y819W (HSP104 Budding Yeast)
UseTags10X His tagExpressionBacterialMutationChanged Tyr 819 to TrpPromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pGL3-p107 1*2*
Plasmid#32387DepositorInsertp107 promoter (Rbl1 Mouse)
UseLuciferaseTagsLuciferaseExpressionMammalianMutation1st and 2nd E2F binding sites mutated: GCG-->A…PromoterAvailable sinceSept. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pPROEX Hsp104 (773-908)
Plasmid#1316DepositorInsertHsp104 (773-908) (HSP104 Budding Yeast)
UseTags6X His + TEV cleavageExpressionBacterialMutationdeletion of aa1-773PromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pPROEX Hsp104 (361-548)
Plasmid#1317DepositorInsertHsp104 (361-548) (HSP104 Budding Yeast)
UseTags6X His + TEV cleavageExpressionBacterialMutationaa361-548 of Hsp104PromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pET151-Vta1(280-330) S278C
Plasmid#87739PurposeBacterial expression of a VSL domain variant of S. cerevisiae Vta1DepositorInsertVacuolar protein sorting-associated protein Vta1 (VTA1 Budding Yeast)
UseTags6xHis-V5-TEVExpressionBacterialMutationdeleted amino acids 1-277, mutated Ser 278 to CysPromoterT7Available sinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET151-Vps4(81-437)
Plasmid#87734PurposeBacterial expression of N-terminally truncated S. cerevisiae Vps4DepositorInsertVacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
UseTags6xHis-V5-TEVExpressionBacterialMutationdeleted amino acids 1-80PromoterT7Available sinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJC45BS104 L892W
Plasmid#1308DepositorInsertHsp104 L892W (HSP104 Budding Yeast)
UseTags10X His tagExpressionBacterialMutationChanged Leu 892 to TrpPromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pJC45BS104 F130W
Plasmid#1302DepositorInsertHsp104 F130W (HSP104 Budding Yeast)
UseTags10X His tagExpressionBacterialMutationChanged Phe 130 to TrpPromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pJC45BS104 L553W
Plasmid#1306DepositorInsertHsp104 L553W (HSP104 Budding Yeast)
UseTags10X His tagExpressionBacterialMutationChanged Leu 553 to TrpPromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pRS426-CeLACE-1
Plasmid#174523PurposeExpress C.elegans LACE-1 in yeast cellsDepositorInsertlace-1 (C30F12.2 Nematode)
UseTags6xHisExpressionYeastMutationPromoterMET25Available sinceSept. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
MYMH 49
Plasmid#138039PurposeEncodes Ribosome Binding Site (RBS) variant using GFP as a reporter geneDepositorInsertpSEVA-331Bb-8AT-GFP
UseTagsExpressionBacterialMutationAAA GAG GTG AAAPromoterAvailable sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
MYMH 56
Plasmid#138042PurposeEncodes Ribosome Binding Site (RBS) variant using GFP as a reporter geneDepositorInsertpSEVA-331Bb-9GA-GFP
UseTagsExpressionBacterialMutationAAA GAG GAA AAAPromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
MYMH 53
Plasmid#138041PurposeEncodes Ribosome Binding Site (RBS) variant using GFP as a reporter geneDepositorInsertpSEVA-331Bb-9GT-GFP
UseTagsExpressionBacterialMutationAAA GAG GAT AAAPromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
MYMH 51
Plasmid#138040PurposeEncodes Ribosome Binding Site (RBS) variant using GFP as a reporter geneDepositorInsertpSEVA-331Bb-9GC-GFP
UseTagsExpressionBacterialMutationAAA GAG GAC AAAPromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
MYMH 47
Plasmid#138038PurposeEncodes Ribosome Binding Site (RBS) variant using GFP as a reporter geneDepositorInsertpSEVA-331Bb-8AC-GFP
UseTagsExpressionBacterialMutationAAA GAG GCG AAAPromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
MYMH 45
Plasmid#138037PurposeEncodes Ribosome Binding Site (RBS) variant using GFP as a reporter geneDepositorInsertpSEVA-331Bb-8AG-GFP
UseTagsExpressionBacterialMutationAAA GAG GGG AAAPromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only