Addgene

We narrowed to 18,394 results for: Tec;

Showing: 281 - 300 of 18394 results

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pJBL715

Plasmid

#140385

Purpose

Expresses MphR protein under the control of T7-lacO promoter

Depositor

Julius Lucks

Article

Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

Insert

T7-lacO-MphR-T

Use

Tags

6XHis

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pJBL701

Plasmid

#140371

Purpose

Expresses TetR protein under the control of T7-lacO promoter

Depositor

Julius Lucks

Article

Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

Insert

T7-lacO-TetR-T

Use

Tags

6XHis

Expression

Bacterial

Mutation

Promoter

Available since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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PX852

Plasmid

#62884

Purpose

C-term SpCas9 piece of inducible split-4 (Cas9(C)-FKBP split-4).

Depositor

Feng Zhang

Article

Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

Insert

SpCas9 (aa536-1368)

Use

CRISPR

Tags

FKBP12, NLS SV40, and NLS nucleoplasmin

Expression

Mammalian

Mutation

Promoter

CBh

Available since

March 4, 2015

Availability

Academic Institutions and Nonprofits only

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pETNb-nALFA

Plasmid

#159988

Purpose

For the protein expression with ALFA tag at N-terminus in E. coli

Depositor

Motoyuki Hattori

Article

Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

Type

Empty backbone

Use

Tags

MVKYI-8histidine-ALFA tag-HRV3C protease cleavage…

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pFBNb-cALFA

Plasmid

#159990

Purpose

For the protein expression with ALFA tag at C-terminus in insect cells

Depositor

Motoyuki Hattori

Article

Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

Type

Empty backbone

Use

Tags

HRV3C protease cleavage site-ALFA tag-8histidine

Expression

Insect

Mutation

Promoter

Available since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pJBL723

Plasmid

#140393

Purpose

Expresses HucR protein under the control of T7-lacO promoter

Depositor

Julius Lucks

Article

Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

Insert

T7-lacO-HucR-T

Use

Tags

6XHis

Expression

Bacterial

Mutation

Promoter

Available since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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pFastBacDual with PA+Histag

Plasmid

#84870

Purpose

Baculovirus Expression of Bacillus anthracis protective antigen

Depositor

Russell Vance

Article

Rauch et al J Exp Med. 2016 May 2;213(5):657-65. doi

Insert

PA

Use

Baculovirus

Tags

His

Expression

Insect

Mutation

Promoter

Polyhedrin

Available since

Nov. 14, 2016

Availability

Academic Institutions and Nonprofits only

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LV anti-FITC-LaG16-Y186F-T2A-BFP

Plasmid

#194931

Purpose

LV construct encoding Y186F mutant sNRR-based SynNotch receptor

Depositor

John Ngo

Article

Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

Insert

antiFITC-LaG16-sNRR(Y186F)-SynNotch-Gal4-VP64-T2A-BFP

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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LV anti-FITC-LaG16-Y186A-T2A-BFP

Plasmid

#194932

Purpose

LV construct encoding Y186A mutant sNRR-based SynNotch receptor

Depositor

John Ngo

Article

Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

Insert

antiFITC-LaG16-sNRR(Y186A)-SynNotch-Gal4-VP64-T2A-BFP

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pETNb-cALFA

Plasmid

#159989

Purpose

For the protein expression with ALFA tag at C-terminus in E. coli

Depositor

Motoyuki Hattori

Article

Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

Type

Empty backbone

Use

Tags

HRV3C protease cleavage site-ALFA tag-8histidine

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pJBL7025

Plasmid

#128810

Purpose

Fluoride riboswitch-regulated catechol 2,3-dioxygenase

Depositor

Julius Lucks

Article

Thavarajah et al ACS Synth Biol. 2019 Dec 12. doi: 10.102

Insert

Fluoride riboswitch-regulated catechol 2,3-dioxygenase

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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LV anti-FITC-LaG16-R103A-T2A-BFP

Plasmid

#194935

Purpose

LV construct encoding R103A mutant sNRR-based SynNotch receptor

Depositor

John Ngo

Article

Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

Insert

antiFITC-LaG16-sNRR(R103A)-SynNotch-Gal4-VP64-T2A-BFP

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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LV TRE-GFP-TMD-DLL1

Plasmid

#194936

Purpose

LV construct encoding GFP-TMD-DLL1 ligand under dox-inducible TRE promoter and containing a PGK-rtTA-M2-IRES-Puro regulator/resistance marker cassette.

Depositor

John Ngo

Article

Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

Insert

eGFP-(PDGFR)TMD-rDLL1 ICD (WT)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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psAAVS1-T2

Plasmid

#60543

Purpose

GFP reporter for CRISPR/CAS9 and gRNA_AAVS1-T2 activities

Depositor

William Simonds

Article

Zhang et al J Biotechnol. 2014 Sep 2. pii: S0168-165

Insert

ProtoSpacer AAVS1-T2 (AAVS1 Human)

Use

CRISPR; Kan

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 6, 2014

Availability

Academic Institutions and Nonprofits only

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LV anti-FITC-LaG16-Y192A-T2A-BFP

Plasmid

#194933

Purpose

LV construct encoding Y192A mutant sNRR-based SynNotch receptor

Depositor

John Ngo

Article

Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

Insert

antiFITC-LaG16-sNRR(Y192A)-SynNotch-Gal4-VP64-T2A-BFP

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 1, 2023

Availability

Academic Institutions and Nonprofits only

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pJBL713

Plasmid

#140383

Purpose

Expresses CtcS protein under the control of T7-lacO promoter

Depositor

Julius Lucks

Article

Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

Insert

T7-lacO-CtcS-T

Use

Tags

6XHis

Expression

Bacterial

Mutation

Promoter

Available since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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pJBL719

Plasmid

#140389

Purpose

Expresses QacR protein under the control of T7-lacO promoter

Depositor

Julius Lucks

Article

Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

Insert

T7-lacO-QacR-T

Use

Tags

6XHis

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pJBL721

Plasmid

#140391

Purpose

Expresses TtgR protein under the control of T7-lacO promoter

Depositor

Julius Lucks

Article

Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

Insert

T7-lacO-TtgR-T

Use

Tags

6XHis

Expression

Bacterial

Mutation

Promoter

Available since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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pXL-CAG-ICeuI-Cerulean-2A-sHRPbNLG1

Plasmid

#79912

Purpose

piggyBac transposon vector, Cerulean-2A-sHRPbNLG1

Depositor

Joshua Sanes

Article

Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

Insert

Cerulean-2A-sHRPbNLG1

Use

Piggybac

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pXL-BacII-CAG-Zeo-t2A-sHRPaNRX3beta

Plasmid

#79913

Purpose

piggyBac transposon vector, Zeocin-2A-sHRPaNRX3beta

Depositor

Joshua Sanes

Article

Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

Insert

Zeocin-t2A-sHRPbNRX3beta

Use

Piggybac

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 21, 2016

Availability

Academic Institutions and Nonprofits only

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