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Plasmid#127200PurposeProgesterone inducible expression of KeyA-BFPDepositorInsertpPAB1-Z3PM-tTDH1-pZ3-key_A_e18-mTagBFP2-tENO1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pAN1735
Plasmid#127204PurposeDual inducible expression of SynTF-mScarlet-degronSwitchA and KeyA-BFP-NLSDepositorInsertpGal1-SynTF-mScarlet-degronSwitch_A_t12-tSSA1-pZ3-key_A_e18-mTagBFP2-NLS(SV40)-tENO1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Lrrc40-trunc-IRES-BFP
Plasmid#117268PurposeRetroviral overexpression of Lrrc40 - truncated version with seemingly same DE genes in Th2 as MSCV-Lrrc40-IRES-BFPDepositorInsertLrrc40 (Lrrc40 Mouse)
UseRetroviralTagsExpressionMammalianMutationThe insert is truncated - deletion of bp 334-407 …PromoterAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOIMars1CyN
Plasmid#75221PurposeMammalian cell surface expression of fluorogen activating protein Mars1CyDepositorInsertss-HA-Mars1Cy-CD80(237-306)
UseTagsHA epitopeExpressionMammalianMutationPromoterCMVAvailable sinceMay 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
lenti-sgRNA(MS2)_zeo_hCYP3A4_enhancer_guide1
Plasmid#176839PurposeTo recruit SAM complex to human CYP3A4 proximal enhancer.DepositorInsertHuman CYP3A4 proximal enhancer sgRNA for activation
UseTagsExpressionMammalianMutationPromoterU6Available sinceNov. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-OptoFGFR1-D387A
Plasmid#59778PurposeExpresses optoFGFR1 with mutation in PHR to suppress homointeraction, thus cannot be activated by lightDepositorInsertOptoFGFR1-D387A (FGFR1 Mustard Weed, Human)
UseTagsmCitrineExpressionMammalianMutationPromoterCMVAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-con/fon DREADD Gq-mCherry
Plasmid#200661PurposeConstruct for chemogenetically activating and visualizing subpopulations of neurons in the retinaDepositorInsertDREADD Gq-mCherry
UseAAV, Cre/Lox, and Mouse Targeting ; IntrsectTagsmCherryExpressionMammalianMutationPromoterhSynAvailable sinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMIEG3-NFAT2
Plasmid#40351DepositorInsertNFAT2 (NFATC1 Mouse)
UseRetroviralTagsIRES eGFPExpressionMammalianMutationPromoterAvailable sinceSept. 28, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBabe-Sik-YF
Plasmid#210723PurposeConstruct for stable expression of constitutively active mouse Ptk6 (Sik) in mammalian cells.DepositorInsertProtein tyrosine kinase 6 (Ptk6 Mouse)
UseTagsExpressionMammalianMutationTyrosine 447 changed to PhenylalaninePromoterAvailable sinceJan. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMCGL
Plasmid#29307DepositorInsertGlucosamine-6-phosphate activated ribozyme
UseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMCGL-T7Mutant
Plasmid#32245DepositorInsertGlucosamine-6-phosphate activated ribozyme
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T2 RANK-L
Plasmid#108571PurposeEncodes C-terminal part of RANKL (aa158-aa316) with a GST N-terminal tagDepositorInsertRANKL (receptor activator of nuclear factor kappa-B ligand) (Tnfsf11 Mouse)
UseTagsGSTExpressionMammalianMutationaa158-aa316PromoterPtac (trp/lac)Available sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDF1-WT_RASA2-EF1-Puro
Plasmid#114096PurposeExpress RASA2 in mamalian cellsDepositorInsertRASA2 (RASA2 Human)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceOct. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDF1-S400F_RASA2-EF1-Puro
Plasmid#114098PurposeExpress mutant RASA2 in mamalian cellsDepositorInsertRASA2 (RASA2 Human)
UseLentiviralTagsFlagExpressionMammalianMutationS400FPromoterCMVAvailable sinceApril 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDF1-R310_RASA2-EF1-Puro
Plasmid#114097PurposeExpress mutant RASA2 in mamalian cellsDepositorInsertRASA2 (RASA2 Human)
UseLentiviralTagsFlagExpressionMammalianMutationR310*PromoterCMVAvailable sinceOct. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
61427-sgHtt_10
Plasmid#157861PurposeExpresses a sgRNA targeting mouse Htt promoter for gene activation using the SAM systemDepositorInsertHtt (Htt Mouse)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
61427-sgHtt_6
Plasmid#157860PurposeExpresses a sgRNA targeting mouse Htt promoter for gene activation using the SAM systemDepositorInsertHtt (Htt Mouse)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV hUbC- VP64-dCas9-VP64-T2A- GFP(No LoxP sites)
Plasmid#66708Purposeexpresses VP64-dCas9-VP64, same plasmid as Addgene 59791 but with LoxP sites removedDepositorInsertS.Pyogenes VP64-dCas9-VP64
UseLentiviral and Synthetic BiologyTagsVP64ExpressionMutationD10A and H840APromoterhUbCAvailable sinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-flex-ChR2 (E123T/T159C)-EYFP
Plasmid#135176PurposeAAV expression of TRE(TRE3G)-driven, Cre-dependent, humanized channelrhodopsin E123T/T159C mutant fused to EYFP for optogenetic activation.DepositorInsertshChR2
TRE3G
UseAAV and Cre/LoxTagsEYFP (C terminal on insert)ExpressionMammalianMutationE123T and T159CPromoterTRE3GAvailable sinceJan. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNANOG-Luc
Plasmid#25900DepositorInsertNANOG promoter (NANOG Human)
UseTagsLuciferaseExpressionMammalianMutationNonePromoterAvailable sinceAug. 5, 2010AvailabilityAcademic Institutions and Nonprofits only