Addgene

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Showing: 2961 - 2980 of 10598 results

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pMJ824

Plasmid

#39314

Depositor

Jennifer Doudna

Article

Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

Insert

Cas9

Use

CRISPR

Tags

GST and His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only

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pT2ST

Plasmid

#59384

Purpose

Template plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a trimethoprim resistance gene.

Depositor

Shelley Copley

Article

Kim et al BMC Biotechnol. 2014 Sep 25;14(1):84.

Insert

TT-ISceI-dfrA

Use

Template

Tags

Expression

Mutation

Promoter

Available since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1 Stu2 1-306

Plasmid

#38315

Depositor

Anthony Hyman

Article

Widlund et al Mol Biol Cell. 2012 Sep 19.

Insert

5' 918 bp from STU2 ORF (STU2 Budding Yeast)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

Aug. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pMito.iGABASnFR.F102G

Plasmid

#112176

Purpose

GABA Sensor

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

Insert

iGABASnFR.F102G

Use

Tags

Expression

Mammalian

Mutation

F102G

Promoter

Available since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-Ace2_D92N_E199V-mRuby3

Plasmid

#129257

Purpose

Express Ace2_D92N_E199V-mRuby3 in mammalian cells

Depositor

Eric Schreiter

Article

Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

Insert

Ace2_D92N_E199V-mRuby3

Use

Tags

Expression

Mammalian

Mutation

D92N, E199V

Promoter

CAG

Available since

Aug. 16, 2019

Availability

Academic Institutions and Nonprofits only

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RV-mCD4-HH5-1

Plasmid

#124961

Purpose

Express the Helicobacter hepaticus specific TCRa/b HH5-1 in T cell hybridoma cells

Depositor

Dan Littman

Article

Xu et al Nature. 2018 Feb 15;554(7692):373-377. d

Insert

HH5-1

Use

retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 6, 2019

Availability

Academic Institutions and Nonprofits only

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pCIG3 B

Plasmid

#132942

Purpose

MLV packaging plasmid for B-tropic MLV gag-pol expression

Depositor

Jeremy Luban

Article

Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

Insert

B-tropic MLV gag-pol

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 7, 2019

Availability

Academic Institutions and Nonprofits only

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Myo 1b-myc

Plasmid

#135064

Purpose

Expression of rat Myo 1b (Myr 1) in mammalian cells. C-terminal myc-tag.

Depositor

Martin Bähler

Article

Ruppert et al J Cell Biol. 1993 Mar;120(6):1393-403. d

Insert

Myo 1b with 5' UTR (Myo1b Rat)

Use

Tags

Linker and Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 28, 2020

Availability

Academic Institutions and Nonprofits only

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Tol2-krt4-RFP

Plasmid

#97149

Purpose

As a control of miRNA sponge

Depositor

Qing Deng

Article

Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

Type

Empty backbone

Use

Zebrafish

Tags

Expression

Mutation

Promoter

Available since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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SEPT5 WT

Plasmid

#27272

Depositor

Harish Pant

Article

Amin et al J Neurosci. 2008 Apr 2. 28(14):3631-43.

Insert

Septin 5 (SEPTIN5 Human)

Use

Tags

Myc (MASMQKLISEEDL)

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only
pCAG-GFPmG1

Plasmid

#50444

Purpose

Expression of a EGFP variant in mammalian cells

Depositor

Connie Cepko

Article

Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

Insert

GFPmG1

Use

Tags

Expression

Mammalian

Mutation

E143K, N147Q

Promoter

CAG

Available since

March 20, 2014

Availability

Academic Institutions and Nonprofits only

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pB1H2w2-mutOdd

Plasmid

#18044

Depositor

Scot Wolfe

Article

Noyes et al Nucleic Acids Res. 2008 Mar 10. ():.

Insert

mutant Odd Skipped

Use

Tags

Expression

Bacterial

Mutation

A mutant version of the zinc finger protein Odd S…

Promoter

Available since

June 20, 2008

Availability

Academic Institutions and Nonprofits only

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pMito.iGABASnFR.mRuby3

Plasmid

#112179

Purpose

GABA Sensor

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

Insert

iGABASnFR

Use

Tags

mRuby3

Expression

Mammalian

Mutation

Promoter

Available since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pCS2 xDvl-RFP

Plasmid

#29683

Depositor

Edward De Robertis

Article

Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

Insert

dishevelled (dvl2.L Frog)

Use

Tags

RFP

Expression

Mammalian

Mutation

Promoter

Available since

June 9, 2011

Availability

Academic Institutions and Nonprofits only
pMito.iGABASnFR.F102G.mRuby3

Plasmid

#112180

Purpose

GABA Sensor

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

Insert

iGABASnFR.F102G

Use

Tags

mRuby3

Expression

Mammalian

Mutation

F102G

Promoter

Available since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pLKO.1P shSOD2

Plasmid

#102976

Purpose

Suppression of SOD2

Depositor

Richard Possemato

Article

Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

Insert

shSOD2 (SOD2 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

Available since

March 14, 2018

Availability

Academic Institutions and Nonprofits only

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pCIG3 N

Plasmid

#132941

Purpose

MLV packaging plasmid for N-tropic MLV gag-pol expression

Depositor

Jeremy Luban

Article

Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

Insert

N-tropic MLV gag-pol

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pUAS-Cre

Plasmid

#50797

Purpose

Expression of Cre recombinase under the control of the UAS-promoter

Depositor

Connie Cepko

Article

Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

Insert

Cre recombinase

Use

Multi-species

Tags

Expression

Mutation

Promoter

UAS-hsp70

Available since

March 20, 2014

Availability

Academic Institutions and Nonprofits only

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pTriEx-mCherry-LOV2 V416I

Plasmid

#81045

Purpose

mammalian expression of LOV V416I

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Insert

LOV V416I

Use

Tags

mCherry

Expression

Bacterial, Insect, and Mamm…

Mutation

V416I

Promoter

Available since

Aug. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pCAG-Ace2_D92N_E199V-mNeon-ST

Plasmid

#129261

Purpose

Express soma-localized Ace2_D92N_E199V-mNeon in mammalian cells

Depositor

Eric Schreiter

Article

Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

Insert

Ace2_D92N_E199V-mNeon-ST

Use

Tags

Expression

Mammalian

Mutation

D92N, E199V

Promoter

CAG

Available since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

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