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Plasmid#214337PurposeProtein overexpression in bacteriaDepositorInsertIL18 (IL18 Human)
UseTagsHis-TEVExpressionBacterialMutationPromoterT7Available sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a_Pro-IL-18_rat
Plasmid#214333PurposeProtein overexpression in bacteriaDepositorInsertIL18 (Il18 Rat)
UseTagsHis-TEV and MycExpressionBacterialMutationPromoterT7Available sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-human-pro-IL-18
Plasmid#214317PurposeExpression of gene in mammalian cells by retroviral transductionDepositorInsertIL18 (IL18 Human)
UseRetroviralTagsFlag and MycExpressionMutationPromoterMESVAvailable sinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
NSP3C-FR-2Strep
Plasmid#210312PurposeExpresses the C terminal of SARS-CoV-2 NSP3 in mammalian cellsDepositorInsertC terminal of SARS-CoV-2 NSP3 (ORF1ab )
UseTags2StrepExpressionMammalianMutationPromoterAvailable sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
NSP3C-EGFP
Plasmid#210318PurposeExpresses the C terminal of SARS-CoV-2 NSP3 fused with EGFP tag in mammalian cellsDepositorInsertC terminal of SARS-CoV-2 NSP3 (ORF1ab )
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
SARS-CoV-2 N-2Strep
Plasmid#210376PurposeExpresses SARS-CoV-2 N protein fused with 2Strep in mammalian cellsDepositorInsertSARS-COV-2 N (N SARS-CoV-2)
UseTags2StrepExpressionMammalianMutationPromoterAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
REEP6-2Strep
Plasmid#210351PurposeExpresses REEP6 fused with 2Strep in mammalian cellsDepositorInsertREEP6 (REEP6 Human)
UseTags2StrepExpressionMammalianMutationPromoterAvailable sinceDec. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
FB323
Plasmid#203570PurposeModule for the expression of geneticin resistance and Nanoluciferase, and the expression of firefly luciferase under the Ppaf promoterDepositorInsertPgpdA:Nluc:Ttub::PtrpC:nptII:Ttub::Ppaf:Luc:Ttub
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHIT186
Plasmid#204513PurposeBacterial expression of CSR-1 PAZ domain with 6xHis and maltose binding protein tags for use as an antigenDepositorInsertPAZ domain of csr-1 (C. elegans) (csr-1 Nematode)
UseTags6xHis, MBPExpressionBacterialMutationPromoterT7lacAvailable sinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHIT520
Plasmid#204519PurposeBacterial expression of CEC-8 N-terminal fragment with 6xHis and maltose binding protein tags for in vitro assaysDepositorInsertN-terminal fragment of cec-8 (C. elegans) (Y55B1BR.3 Nematode)
UseTags6xHis, MBPExpressionBacterialMutationPromoterT7lacAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHIT320
Plasmid#204517PurposeBacterial expression of HIM-1 internal fragment with 6xHis and maltose binding protein tags for use as an antigenDepositorInsertInternal fragment of him-1 (C. elegans) (him-1 Nematode)
UseTags6xHis, MBPExpressionBacterialMutationPromoterT7lacAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-ZNF354C_mutant_D17A-V18A
Plasmid#194184PurposeExpresses ZNF354C with mutated KRAB domain (D17A, V18A mutation)DepositorInsertZNF354C (ZNF354C Human)
UseTagsMyc-DDKExpressionMammalianMutationKRAB domain mutant D17A/V18APromoterCMVAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-ZNF354C_mutant_E25A-W26A
Plasmid#194186PurposeExpresses ZNF354C with mutated KRAB domain (E25A, W26A mutation)DepositorInsertZNF354C (ZNF354C Human)
UseTagsMyc-DDKExpressionMammalianMutationKRAB domain mutant E25A/W26APromoterCMVAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET29a(+)::D1141A
Plasmid#192963PurposeTest the activity of the putative aspartyl protease motif DPXGL-(18)-DPXGLDepositorInsertTse5_D1141A
UseTags9xhis + TEV tagExpressionBacterialMutationD1141APromoterAvailable sinceDec. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET29a(+)::9xhis-Tse5
Plasmid#192962PurposeHeterologous expression of Tse5 in E. coli Lemo21 cells for purification of Tse5-CTDepositorInsertTse5
UseTags9xhis + TEV tagExpressionBacterialMutationPromoterAvailable sinceDec. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28-6xhis-sumo-CeMIGA(106-496)
Plasmid#192832PurposeExpress C.elegans MIGA fragment 106-496 in bacteriaDepositorInsertMitoguardin homologue (miga-1 Nematode)
UseTags6xhis sumoExpressionBacterialMutationPromoterT7Available sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJH4140
Plasmid#191479PurposePacr-2s-loxP::EBFP::loxP::Chrimson::wCherry unc-54 3' UTR C.elegans A/B MN expression of Arch RFPDepositorInsertChrimson
UseTagswCherryExpressionWormMutationPromoterPacr-2sAvailable sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJH4143
Plasmid#191531PurposePunc-17-Cre unc-54 3'UTR C.elegans for Cre/LoxDepositorInsertCre
UseCre/LoxTagsno taqExpressionMutationPromoterPunc-17Available sinceDec. 13, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJH2999
Plasmid#191373PurposePunc-25-GCaMP3 ::wCherry unc-54 3' UTR C.elegans DD neurou expression of GCaMP3 RFPDepositorInsertGCaMP3
UseTagswCherryExpressionWormMutationPromoterPunc-25Available sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJH3557
Plasmid#191444PurposePttr-39-Chrimson::wCherry unc-54 3' UTR C.elegans D motor neurou expression of Chrimson RFPDepositorInsertChrimson
UseTagswCherryExpressionWormMutationPromoterPttr-39Available sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only