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EK0303 SFFV-mCherry-SG(Bdnf.s3)SG-EGFP (FLP-IN)
Plasmid#191180PurposeNegative control sensor in human cells.DepositorInsertmCherry:Bdnf.s3:EGFP (negative control)
UseTagsExpressionMammalianMutationWTPromoterSFFVAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
3XHalo_GFP_TRF1#1
Plasmid#164644Purposetag TRF1 with GFP and HaloDepositorInsertTRF1 (TERF1 Human)
UseCre/LoxTagsExpressionMammalianMutationPromoterCAGAvailable sinceMarch 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
EIF2AK4 gRNA (BRDN0001146469)
Plasmid#75876Purpose3rd generation lentiviral gRNA plasmid targeting human EIF2AK4DepositorInsertEIF2AK4 (EIF2AK4 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAG-NLS-HA-Bxb1
Plasmid#51271PurposeComponent of the Bxb1-rcCre binary site-specific recombinase system, expression of the site-specific recombinase Bxb1 (mouse codon-optimized)DepositorInsertNLS-HA-Bxb1
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAd-Track Flag HA PGC-1 alpha
Plasmid#14426DepositorInsertPGC-1 alpha (Ppargc1a Mouse)
UseAdenoviralTagsFlag and HAExpressionMammalianMutationLast four amino acids of PGC-1a removed to make t…PromoterAvailable sinceFeb. 23, 2007AvailabilityAcademic Institutions and Nonprofits only -
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Lenti-sgNT3/Cre
Plasmid#89654PurposeExpresses a non-targeting gRNA and Cre-recombinaseDepositorInsertnon-targeting gRNA
UseCre/Lox and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFM699
Plasmid#74636Purposealias pFA6a-mCherry-natNT2 - vector for C-terminal tagging of yeast genes with mCherry and nourseothricin selection markerDepositorInsertmCherry
UseTagsExpressionYeastMutationPromoternoneAvailable sinceJune 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
T4 Lysozyme L99A WT*
Plasmid#18476DepositorInsertT4 Lysozyme (e Bacteriophage T4)
UseTagsExpressionBacterialMutationC54T C97A L99A WT*PromoterAvailable sinceAug. 28, 2008AvailabilityAcademic Institutions and Nonprofits only -
pU6T7
Plasmid#71462PurposeExpresses gRNA from hybrid human U6/T7 promoter for both cellular expression and in vitro transcription from same promoter (2 Gs at initiation)DepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterial and MammalianMutationPromoterhuman U6/phage T7Available sinceFeb. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
tdTomato-Calreticulin-N-16
Plasmid#58074PurposeLocalization: Endoplasmic Reticulum, Excitation: 554, Emission: 581DepositorInsertCalreticulin (CALR Human)
UseTagstdTomatoExpressionMammalianMutationPromoterCMVAvailable sinceDec. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-TARG1
Plasmid#172594PurposeFor purification (from E.coli) of His-tagged TARG1DepositorInsertTARG1 (OARD1 Human)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceApril 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)-SNAP-CLIP
Plasmid#127180PurposeBacterial expression of the fused self-labeling tags SNAP-tag and CLIP-tagDepositorInsertSNAP-CLIP
UseTagsHisTag and StrepTagExpressionBacterialMutationPromoterT7Available sinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-SynI-CreOn-FlpOff-mNaChBacMUT-P2A-EGFP
Plasmid#176281PurposeViral vector for co-expression of non-functional NaChBac and EGFP in cells expressing Cre AND NOT Flp driven by the human Synapsin promoter.DepositorInsertmNaChBacMUT-P2A-EGFP
UseAAV and Cre/LoxTagsExpressionMammalianMutationE191KPromoterhuman Synapsin IAvailable sinceJan. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBabe-CD44v6
Plasmid#107970Purposeexpression of CD44 variantDepositorInsertmCD44v6 (Cd44 Mouse)
UseRetroviralTagsExpressionMammalianMutationsplice variant; see Depositor Comments belowPromoterAvailable sinceJan. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJZC588
Plasmid#62315PurposesgRNA with 2x MS2 (wt+f6) for yeast cellsDepositorInsertsgRNA + 2x MS2 (wt+f6) binding module
UseTagsExpressionYeastMutationPromoterSNR52Available sinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
LV anti-FITC-LaG16-Y186A-T2A-BFP
Plasmid#194932PurposeLV construct encoding Y186A mutant sNRR-based SynNotch receptorDepositorInsertantiFITC-LaG16-sNRR(Y186A)-SynNotch-Gal4-VP64-T2A-BFP
UseLentiviral and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
EIF2AK4 gRNA (BRDN0001145948)
Plasmid#75875Purpose3rd generation lentiviral gRNA plasmid targeting human EIF2AK4DepositorInsertEIF2AK4 (EIF2AK4 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBW2287_pCAG-PV1-PYL-L1-FlpO-397C-NLS-BGHpA
Plasmid#108770PurposeExpresses aa397 to C-terminus of Flp recombinase fused to PYL CIDDepositorInsertFlp recombinase fragment aa397 to C-terminus
UseCre/Lox and Synthetic BiologyTagsNLS and PYLExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only
