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pSc1-puro
Plasmid#80438PurposeExpresses eGFP along with an U6 promoter driven gRNA which cleaves the plasmid in-cell, and also confers resistance to puromycinDepositorInsertssp Cas9 gRNA
eGFP
Puromycin resistance
UseCRISPRTagsExpressionMammalianMutationPromoterCMV, U6, and hPGKAvailable sinceSept. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
GFP-α-cateninΔβH
Plasmid#229705PurposeTransient expression of GFP-alpha-catenin-delta-beta-H in mammalian cellsDepositorInsertCTNNA1 (alpha-catenin) Human (CTNNA1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMV promoterAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
GFP-α-cateninV-
Plasmid#229696PurposeTransient expression of GFP-alpha-cateninV- in mammalian cellsDepositorInsertCTNNA1 (alpha-catenin) Human (CTNNA1 Human)
UseTagsEGFPExpressionMammalianMutationL344PPromoterCMV promoterAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
GFP-α-cateninA+
Plasmid#229697PurposeTransient expression of GFP-alpha-cateninA+ in mammalian cells; enhanced actin bindingDepositorInsertCTNNA1 (alpha-catenin) Human (CTNNA1 Human)
UseTagsEGFPExpressionMammalianMutationamino acids 670-673, RAIM--> GSGSPromoterCMV promoterAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
GFP-α-cateninA+ΔβH
Plasmid#229704PurposeTransient expression of GFP-alpha-cateninA+deltabetaH in mammalian cellsDepositorInsertCTNNA1 (alpha-catenin) Human (CTNNA1 Human)
UseTagsEGFPExpressionMammalianMutationamino acids 670-673, RAIM--> GSGSPromoterCMV promoterAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
GFP-α-cateninA-
Plasmid#229698PurposeTransient expression of GFP-alpha-cateninA- in mammalian cellsDepositorInsertCTNNA1 (alpha-catenin) Human (CTNNA1 Human)
UseTagsEGFPExpressionMammalianMutationL785A, I792A, V796APromoterCMV promoterAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
GFP-α-cateninWT
Plasmid#229709PurposeTransient expression of GFP-alpha-cateninWT in mammalian cellsDepositorInsertCTNNA1 (alpha-catenin) Human (CTNNA1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMV promoterAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pNES-SET-EMD-EGFP (C-NES-SET-EMD)
Plasmid#217770PurposeFluorescent reporter for ceramide (putative, mammalian expression)DepositorInsertSET (SET Human)
UseTagsEGFPExpressionMammalianMutationEMD domain (aa 70-226) plus nuclear export sequen…PromoterCMVAvailable sinceJune 12, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
p2X-KSR1-CA3-EGFP (2x-C-KSR)
Plasmid#217756PurposeFluorescent reporter for ceramide (putative, mammalian expression)DepositorInsertKinase suppressor of ras 1 CA3 domain (KSR1 Human)
UseTagsEGFPExpressionMammalianMutationTwo tandem CA3 domain (aa 317-400) 1st linker GG…PromoterCMVAvailable sinceJune 10, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pKSR1-CA3-EGFP (C-KSR)
Plasmid#217753PurposeFluorescent reporter for ceramide (mammalian expression)DepositorInsertKinase suppressor of ras 1 CA3 domain (KSR1 Human)
UseTagsEGFPExpressionMammalianMutationCA3 domain aa 317-400 plus vector-based linker LE…PromoterCMVAvailable sinceJune 10, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
Stx5L(m55V)-GFP
Plasmid#209845PurposeExpresses Stx5 M55V mutant fused to GFP in mammalian cellsDepositorInsertSTX5 (STX5 Human)
UseTagseGFPExpressionMammalianMutationChanged Methionine 55 to Valine, causes loss of S…PromoterCMVAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-EGFP-N3-CYB5R3 K214R
Plasmid#204570PurposeExpresses mCherry-EGFP-tagged CYB5R3 K214R in mammalian cellsDepositorInsertCYB5R3 (CYB5R3 Human)
UseTagsExpressionMammalianMutationK214RPromoterAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-EGFP-N3-CYB5R3
Plasmid#204569PurposeExpresses mCherry-EGFP-tagged CYB5R3 in mammalian cellsDepositorInsertCYB5R3 (CYB5R3 Human)
UseTagsExpressionMammalianMutationnonePromoterAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGFP-nE-mHP1alpha
Plasmid#181895PurposeFluorescently tagged HP1alpha, serines in N-terminal extension (NTE) replaced by glutamatesDepositorInsertnE-mHP1alpha (Cbx5 Mouse)
UseTagsGFPExpressionMammalianMutationS11E, S12E, S13E, S14EPromoterCMVAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGFP-PGL-1-mHP1alpha
Plasmid#181899PurposeFluorescently tagged HP1alpha fused to PGL-1DepositorInsertPGL-1-mHP1alpha (Cbx5 Mouse, Nematode)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
GFP-Cstem(7A)-Tac(5A)
Plasmid#162499PurposeExpresses GFP tagged Tac mutant in mammalian cells. The five O-glycosylation sites in the stem region are mutated, and the stem region of CD8a with mutations is inserted between GFP and Tac(5A).DepositorInsertUseTagsGFPExpressionMammalianMutationThe five theronine and two serine residues are mu…PromoterAvailable sinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEZYegfp
Plasmid#18671PurposepEGFPC4 converted Gateway Destination vector for expressing EGFP tagged fusion proteins in mammalian cellsDepositorTypeEmpty backboneUseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 26, 2008AvailabilityAcademic Institutions and Nonprofits only -
pRSET a sfGCaMP6s
Plasmid#100022PurposeBacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. cpEGFP replaced with a superfolder cpGFP variant.DepositorInsertsfGCaMP6s
UseTags6x HIS tag and Xpress_EK tagExpressionBacterialMutationcpEGFP replaced with superfolder cpGFPPromoterT7Available sinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET15b-eGO-Matryoshka-LE-LP
Plasmid#100017PurposeBacterial expression of fluorescent protein casette - cpEGFP with nested LSSmOrange and LE N-term and LP C-term amino acid linkersDepositorInsertcpEGFP-LSSmOrange
UseTags6x HIS tagExpressionBacterialMutationLeu and Glu N-term linker and Leu and Pro C-term …PromoterT7Available sinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only
