Addgene

We narrowed to 5,239 results for: pCas

Showing: 2941 - 2960 of 5239 results

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pFH100

Plasmid

#128205

Purpose

improved sgRNA backbone for SpCas9, combine with AtU6-26 promoter module (pFH35)

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

improved sgRNA backbone for SpCas9, combine with AtU6-26 promoter module (pFH35)

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pSL1315

Plasmid

#129527

Purpose

pDEF-SpCas9, Best (-458) Vector for CRISPRi of PyALBA4, PbEF1a promoter, 1st Generation

Depositor

Scott Lindner

Article

Walker et al J Biol Chem. 2019 Jun 14;294(24):9555-95

Insert

dSpCas9

Use

Plasmodium, rodent-infectious species

Tags

NLS, 1xHA

Expression

Mutation

Promoter

Available since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pSK-Spsg

Plasmid

#214351

Purpose

Expresses cloning backbone for SpCas9 sgRNA

Depositor

Yongming Wang

Article

Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

Insert

SpCas9 tracrRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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3919-32

Plasmid

#176496

Purpose

pCAG-puro-T2A-TetON3G-polyA

Depositor

Albert La Spada

Article

Hsu et al Nat Commun. 2018 Mar 5;9(1):942. doi: 10

Insert

puroR-T2A-TetOn3G

Use

Tags

Expression

Mammalian

Mutation

Promoter

TRE

Available since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pFA197

Plasmid

#149602

Purpose

(parent plasmid pCFB262) pXII - 4 -LoxP -SpHIS5 -209bp - pCYC1 -PcaO -108 -yeGFP

Depositor

Michael Jensen

Article

Ambri et al ACS Synth Biol. 2020 Feb 21;9(2):218-226

Insert

yeGFP

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

PcaO_1O8

Available since

Dec. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pSL1211

Plasmid

#129521

Purpose

pDEF-SpCas9::GFP, For deletion of pyalba4, PyGAPDH promoter, 2nd Generation

Depositor

Scott Lindner

Article

Walker et al J Biol Chem. 2019 Jun 14;294(24):9555-95

Insert

SpCas9

Use

Plasmodium, rodent-infectious species

Tags

3XFlag-NLS

Expression

Mutation

Promoter

Available since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pFH70

Plasmid

#128190

Purpose

Level1 ZmUBIp::nSpCas9-PmCDA (Arabidopsis codon optimized):NOSt

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

ZmUBIp::nSpCas9-PmCDA (Arabidopsis codon optimized):NOSt

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pEPOR0SP0014

Plasmid

#117524

Purpose

Level0 Golden Gate part: CDS, SpCas9-KA

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

SpCas9-KA (with stop codon)

Use

CRISPR

Tags

Expression

Mutation

K855A

Promoter

Available since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR0SP0009

Plasmid

#117522

Purpose

Level0 Golden Gate part: CDS, SpCas9-p (no stop codon)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

SpCas9-p (no stop codon)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pICSL90005

Plasmid

#117520

Purpose

Level0 Golden Gate part: CDS, SpCas9-h (no stop codon)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

SpCas9-h (no stop codon)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pX330A-1x2

Plasmid

#58766

Purpose

Expresses Cas9 nuclease and gRNA

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 nuclease

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CBh

Available since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pC0052-REPAIR non-targeting guide clone into pC0043

Plasmid

#103868

Purpose

Non-targeting guide RNA for use with PspCas13b. Useful as a control for RNA editing and RNA knockdown experiments.

Depositor

Feng Zhang

Article

Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

Insert

non-targeting guide RNA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pMCRi

Plasmid

#207524

Purpose

Plasmid for CRISPRi; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→ Sp dCas9, PEM7 →sgRNA; SmR/SpR

Depositor

Pablo Ivan Nikel

Article

Kozaeva et al Nucleic Acids Res. 2024 Jan 5:gkad1236.

Insert

xylS (cured of BsaI-sites), Pm→ Sp dCas9, PEM7 →sgRNA; SmR/SpR

Use

Pseudomonas vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pDGE1109

Plasmid

#202773

Purpose

CRISPR-KO with TRE2 exonuclease co-expression in Arabidopsis thaliana

Depositor

Johannes Stuttmann

Article

Ordon et al Plant Methods. 2023 Mar 28;19(1):30. doi

Inserts

FAST marker
pRPS5a:TREX2-zCas9_ttriple
ccdB cassette (BsaI-excisable)

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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sABE v3.22_C

Plasmid

#209554

Purpose

pCMV-SV40_NLS-2xFKBP3-TadA-8e(77-167)-SpCas9(D10A)-SV40_NLS-BGHpA

Depositor

Xue Gao

Article

Zeng et al Nat Commun. 2023 Sep 11;14(1):5573. doi:

Insert

2xFKBP-TadA-8e(77-167)-SpCas9(D10A)

Use

Tags

Expression

Mammalian

Mutation

Promoter

pCMV

Available since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pDGE1108

Plasmid

#202772

Purpose

CRISPR-KO in Arabidopsis thaliana

Depositor

Johannes Stuttmann

Article

Ordon et al Plant Methods. 2023 Mar 28;19(1):30. doi

Inserts

FAST marker
pRPS5a:zCas9_ttriple
ccdB cassette (BsaI-excisable)

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pFB-Flag-MyoD-MyoD

Plasmid

#25992

Depositor

Jeffrey Dilworth

Article

Dilworth et al Proc Natl Acad Sci U S A. 2004 Aug 10. 1

Insert

MyoD-MyoD homodimer (Myod1 Mouse)

Use

Baculovirus transfer vector

Tags

Flag

Expression

Mutation

Promoter

Available since

Sept. 8, 2010

Availability

Academic Institutions and Nonprofits only
pCBI-LexA-CIB1-biLINuS

Plasmid

#192605

Purpose

Plasmid for constitutive expression of LexA-CIB1-biLINuS.

Depositor

Yingxiao Wang

Article

Huang et al Sci Adv. 2020 Feb 19;6(8):eaay9209. doi:

Insert

LexA-CIB1-biLINuS

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

pCAGGS

Available since

Nov. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pH-A3A-PBE

Plasmid

#119774

Purpose

Conversion of C to T in rice plants transformation

Depositor

Caixia Gao

Article

Zong et al Nat Biotechnol. 2018 Oct 1. pii: nbt.426

Insert

APOBEC3A-nCas9-NLS-UGI-NLS

Use

Tags

NLS (flanking UGI)

Expression

Plant

Mutation

nCas9 has a mutation D10A in wild type SpCas9

Promoter

maize Ubiquitin-1

Available since

Sept. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pmCherry_gRNA

Plasmid

#80457

Purpose

gRNA expression vector with mCherry transfection control

Depositor

Ervin Welker

Article

Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

Inserts

sp Cas9 gRNA
mCherry

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6 and chicken β-actin promoter

Available since

Aug. 11, 2016

Availability

Academic Institutions and Nonprofits only

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