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Showing: 2941 - 2960 of 3269 results

  1. pEH_AAV_TERT_prom_C7C_1.8kb

    Plasmid
    #183079
    Purpose
    rAAV transfer plasmid with ITRs flanking: (1) TERT wildtype promoter and exon 1 C7C (C21>T21), 1.8kb homologous recombination donor template with ~900 bp homology arms, and (2) PGK-Puro.
    Depositor
    Aviv Regev
    Article
    Hodis et al Science. 2022 Apr 29;376(6592):eabi8175.
    Insert
    TERT wildtype promoter and exon 1 C7C (C21>T21), 1.8kb homologous recombination donor template with ~900 bp homology arms (TERT Human)
    Use
    AAV; Homologous recombination donor template
    Tags
    Expression
    Mutation
    Wildtype promoter, Exon 1 C7C (C21>T21) silent…
    Promoter
    None for primary insert (Though partial TERT prom…
    Available since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    183079-plasmid-map-sequence-id-363414

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  2. pEH_AAV_TERT_prom_-146C>T_C7C_1.8kb

    Plasmid
    #183081
    Purpose
    rAAV transfer plasmid with ITRs flanking: (1) TERT -146C>T promoter and exon 1 C7C (C21>T21), 1.8kb homologous recombination donor template with ~900 bp homology arms, and (2) PGK-Puro.
    Depositor
    Aviv Regev
    Article
    Hodis et al Science. 2022 Apr 29;376(6592):eabi8175.
    Insert
    TERT -146C>T promoter and exon 1 C7C (C21>T21), 1.8kb homologous recombination donor template with ~900 bp homology arms (TERT Human)
    Use
    AAV; Homologous recombination donor template
    Tags
    Expression
    Mutation
    -146C>T promoter, Exon 1 C7C (C21>T21) sile…
    Promoter
    None for primary insert (Though partial TERT prom…
    Available since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    183081-plasmid-map-sequence-id-363415

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  3. pEH_AAV_BRAF_ex15_V600E_S607S_1.8kb

    Plasmid
    #183078
    Purpose
    rAAV transfer plasmid with ITRs flanking: (1) BRAF V600E (T>A), S607S (TCC>AGT), 1.8kb homologous recombination donor template centered on BRAF exon 15 with ~900 bp homology arms, and (2) PGK-Puro.
    Depositor
    Aviv Regev
    Article
    Hodis et al Science. 2022 Apr 29;376(6592):eabi8175.
    Insert
    BRAF Exon 15, 1.8kb homologous recombination donor, ~900 bp homology arms (BRAF Human)
    Use
    AAV; Homologous recombination donor template
    Tags
    Expression
    Mutation
    V600E (T>A), S607S (TCC>AGT)
    Promoter
    None for primary insert. PGK for puromycin resist…
    Available since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    183078-plasmid-map-sequence-id-363420

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  8. PB513B-1/TRE-hHNF1α-hHNF3β-hCEBPα-hCEBPβ-hCEBPγ-EF1α-Puro

    Plasmid
    #199550
    Purpose
    PiggyBac-based transposon vector plasmid which encodes the expression units of liver-enriched transcription factor genes (hHNF1α-hHNF3β-hCEBPα-hCEBPβ-hCEBP-γ) under control of the TRE/PCMVmin promoter
    Depositor
    Masamichi Kamihira
    Article
    Kitano et al Cells. 2022 Apr 1;11(7):1194. doi: 10.33
    Insert
    hHNF1α-hHNF3β-hCEBPα-hCEBPβ-hCEBPγ (HNF1A, FOXA2, CEBPB, CEBPA, CEBPG Human)
    Use
    Piggybac transposon vector
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE+CMVmin promoter
    Available since
    Aug. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    199550-plasmid-map-sequence-id-405449

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  10. pFBD-BirA_CDC20:161-P477

    Plasmid
    #210888
    Purpose
    Insect expression of human CDC20 fragment S161 to P477. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.
    Depositor
    Cheryl Arrowsmith
    Article
    Ackloo et al bioRxiv 2024.03.03.583197
    Insert
    CDC20:S161-P477 (CDC20 Human)
    Use
    Tags
    GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG
    Expression
    Insect
    Mutation
    wild type
    Promoter
    Polyhedrin
    Available since
    March 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    210888-plasmid-map-sequence-id-424502

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  11. pBIG2abc_hsHAUScomplex_FL

    Plasmid
    #202203
    Purpose
    Baculoviral transfer vector to co-express 8 subunits of human augmin complex
    Depositor
    Thomas Surrey
    Article
    Ustinova et al J Cell Sci. 2023 Jun 15;136(12):jcs26109
    Inserts
    HAUS1 (HAUS1 Human, codon-optimized form for expression in Trichoplusia ni cells)
    HAUS3 (HAUS3 Human, codon-optimized form for expression in Trichoplusia ni cells)
    HAUS2 (HAUS2 Human, codon-optimized form for expression in Trichoplusia ni cells)
    HAUS6 (HAUS6 Human, codon-optimized form for expression in Trichoplusia ni cells)
    HAUS7 (HAUS7 Human, codon-optimized form for expression in Trichoplusia ni cells)
    HAUS4 (HAUS4 Human, codon-optimized form for expression in Trichoplusia ni cells)
    HAUS5 (HAUS5 Human, codon-optimized form for expression in Trichoplusia ni cells)
    HAUS8 (HAUS8 Human, codon-optimized form for expression in Trichoplusia ni cells)
    Use
    Tags
    -mGFP-TEV-TwinStrep and His-tag (6x)
    Expression
    Insect
    Mutation
    Promoter
    polyhedrin promoter
    Available since
    Sept. 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    202203-plasmid-map-sequence-id-407808

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  12. JGI_018101

    Plasmid
    #158920
    Purpose
    Hydrocarbon Structures for Fuels and Chemicals
    Depositor
    Joint Genome Institute
    Insert
    WP_028327376_1
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    158920-plasmid-map-sequence-id-315979

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  13. JGI_017953

    Plasmid
    #158881
    Purpose
    Hydrocarbon Structures for Fuels and Chemicals
    Depositor
    Joint Genome Institute
    Insert
    WP_063765283_1
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    158881-plasmid-map-sequence-id-317629

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  14. Quas:GFPpA;Betacrystallin:CFP

    Plasmid
    #81181
    Purpose
    Replaces #61376. QUAS driving GFP with beta crystalline CFP eye marker
    Depositor
    Marnie Halpern
    Insert
    QUAS:GFP;beta crys:CFP
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 22, 2016
    Availability
    Academic Institutions and Nonprofits only
    81181-plasmid-map-sequence-id-160381

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  15. MTMR6A

    Plasmid
    #42483
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    MTMR6A
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    See Comments
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  16. TNKS2A

    Plasmid
    #42491
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    TNKS2A
    Use
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    See Comments
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  17. SEC14L4

    Plasmid
    #73233
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    SEC14L4 (SEC14L4 Human)
    Use
    Tags
    His-6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    73233-plasmid-map-sequence-id-137211

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  18. TNKS2A

    Plasmid
    #42463
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    TNKS2A (TNKS2 Human)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    See Comments
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  19. pAc5.1B-EGFP-DmRigor-mortis_AA

    Plasmid
    #148203
    Purpose
    Insect Expression of DmRigor-mortis
    Depositor
    Elisa Izaurralde
    Insert
    DmRigor-mortis (rig Fly)
    Use
    Tags
    Expression
    Insect
    Mutation
    25 silent and 12 non-silent mutations compared to…
    Promoter
    Available since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    148203-plasmid-map-sequence-id-290651

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  20. pAc5.1B-lambdaN-HA-DmRigor-mortis_AA

    Plasmid
    #148218
    Purpose
    Insect Expression of DmRigor-mortis
    Depositor
    Elisa Izaurralde
    Insert
    DmRigor-mortis (rig Fly)
    Use
    Tags
    Expression
    Insect
    Mutation
    25 silent and 12 non-silent mutations compared to…
    Promoter
    Available since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    148218-plasmid-map-sequence-id-290666

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Showing: 2941 - 2960 of 3269 results