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Plasmid#223959PurposeDual fluorescent reporter for histone H2B and for APC/C activityDepositorUseLentiviralTagsiRFP670 and mCeruleanExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2024AvailabilityAcademic Institutions and Nonprofits only
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Mito-CanlonicSF/pCMV-myc-mito
Plasmid#224465PurposeTo explore mitochondrial lactate dynamicsDepositorInsertCanlonicSF
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 19, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
Human Transporter Knockout Library with UMIs
Pooled Library#221409PurposegRNA library to test the impact of transporters on biological processesDepositorSpeciesHomo sapiensUseCRISPR and LentiviralAvailable sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
Anti-Kv3.3 K+ channel [N375/67R-2b]
Plasmid#219487PurposeMammalian Expression Plasmid of anti-Kv3.3 K+ channel (Mouse) subclass-switched IgG2b R-mAb. Derived from hybridoma N375/67.DepositorInsertAnti-Kv3.3 K+ channel (Mus musculus) recombinant mouse monoclonal antibody. (Kcnc3 Mouse)
UseTagsExpressionMammalianMutationPromoterDual CMVAvailable sinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPPI4-Strep-SNAP-CTLA-4-His6
Plasmid#223583PurposeProtein purification of human CTLA4 with Twin-Strep, SNAP, GCN4 and 6xHis tag from mammalian cellsDepositorInsertCTLA4 (CTLA4 Human)
UseTagsHis6 and Twin-Strep, SNAPExpressionMammalianMutationPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAPM-miR30-HNRNPK-ts1
Plasmid#174250PurposeHNRNPK knockdownDepositorInsertHNRNPK shRNA (HNRNPK Human)
UseLentiviral and RNAiTagsExpressionMutationPromoterSFFVAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAPM-miR30-HNRNPK-ts3
Plasmid#174252PurposeHNRNPK knockdownDepositorInsertHNRNPK shRNA (HNRNPK Human)
UseLentiviral and RNAiTagsExpressionMutationPromoterSFFVAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG>nls-Cas9-nls-2A-Citrine-EcoRI/NotI
Plasmid#169097PurposeCas9 and Citrine expression in transfected cellsDepositorInsertCas9-2A-Citrine
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
p-Tet-O-RUNX1T1-Puro
Plasmid#221666PurposeExpresses RUNX1T1 in mammalian cellsDepositorInsertRUNX1T1 (RUNX1T1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET22b-ELP[V]60
Plasmid#219406Purpose(VPGVG)60DepositorInsertELP[V]60
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-EGFP-N-IFT80
Plasmid#218731PurposeExpresses C-terminally EGFP-tagged IFT80 in mammalian cellsDepositorInsertIFT80 (IFT80 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCAGAvailable sinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
CCR10-DuET
Plasmid#213197PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertCCR10 (CCR10 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET151-GyrA
Plasmid#216229PurposeS. aureus USA300 GyrA Codon-optimized for E. coliDepositorInsertDNA gyrase
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET151-GyrB
Plasmid#216233PurposeS. aureus USA300 GyrB Codon-optimized for E. coliDepositorInsertDNA gyrase
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBiT3.1-N_RRP9:1-475
Plasmid#211133PurposeMammalian expression of full-length human RRP9. N-terminal HiBiT tag.DepositorInsertRRP9:M1-S475 (RRP9 Human)
UseTagsMVSGWRLFKKISGSSGGSSGExpressionMammalianMutationwild typePromoterCMVAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCF822-recipient_U6-sgRNA-EFS-Cas9-P2A-Puro
Plasmid#211687PurposeU6-sgRNA-EFS-Cas9-P2A-PuroDepositorInsertSpyCas9 and guide RNA recipient vector
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-HA-deLACCO1-NGR
Plasmid#207990PurposeControl biosensor for extracellular L-lactateDepositorInsertdeLACCO1
UseAAVTagsExpressionMutationPromoterCaMKIIAvailable sinceJan. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQE60 R196M wgMDH
Plasmid#204273PurposeBacterial expression of R196M wgMDH with IPTG induction - Active site mutantInsertMDHG_CITLA
UseTags6X HisExpressionBacterialMutationR196MPromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 R130A wgMDH
Plasmid#204266PurposeBacterial expression of R130A wgMDH with IPTG induction - Active site mutantInsertMDHG_CITLA
UseTags6X HisExpressionBacterialMutationR130APromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 D193E wgMDH
Plasmid#204269PurposeBacterial expression of D193E wgMDH with IPTG induction - Active site mutantInsertMDHG_CITLA
UseTags6X HisExpressionBacterialMutationD193EPromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only