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Plasmid#160995PurposeExpress mouse p150 protein with MIR deletion mutationDepositorInsertCHAF1A (Chaf1a Mouse)
UseTagsGFPExpressionMammalianMutationMIR (HP1-interacting domain) deletionPromoterAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFB-D3-ASK1
Plasmid#226619PurposeCo-expression of D3 and ASK1 complex in insect cellsDepositorInsertsUseTagsHis tag followed with three copies of MsybExpressionInsectMutationThe flexible loop in D3 (Q478-D515) can be remove…PromoterPolyhedrinAvailable sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
GFP Cav2.2 W391A pRK5
Plasmid#206084Purposeexpression of rabbit Cav2.2 calcium channel with a N-terminal GFP tag and a mutation (W391A) in the ?1-interacting domain (AID) in the I-II loop of Cav2.2 that prevents Beta subunit bindingDepositorInsertcacna1b (CACNA1B Rabbit)
UseTagsGFPExpressionMammalianMutationW391APromoterCMVAvailable sinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
CSII-TK-MAIP1
Plasmid#203599PurposeLentiviral vector to express MAIP1 in mammalian cellsDepositorInsertmatrix AAA peptidase interacting protein 1 (MAIP1 Human)
UseLentiviralTagsExpressionMammalianMutationWTPromoterTKAvailable sinceOct. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
psicheck2-MAIP1
Plasmid#203603PurposeReporter plasmid carrying MAIP1 3'UTRDepositorInsertmatrix AAA peptidase interacting protein 1 (MAIP1 Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterSV40Available sinceOct. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
Cav2.2 HA W391A pcDNA3
Plasmid#206071Purposeexpression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and a mutation (W391A) in the ?1-interacting domain (AID) in the I-II loop of Cav2.2DepositorInsertcacna1b (CACNA1B Rabbit)
UseTagsExpressionMammalianMutationW391APromoterCMVAvailable sinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-RIPK3[V458D][Q459D][V460D][G461D]-FLAG
Plasmid#199327Purposemutated RHIM motive (interaction active)DepositorInsertReceptor-interacting serine/threonine-protein kinase 3 (RIPK3 Human)
UseTagsFLAGExpressionMammalianMutationchanged V458, Q459,V460 and G461 to aspartic acidPromoterAvailable sinceJune 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmCYFIP1_T
Plasmid#147578PurposeInsect Expression of DmCYFIP1DepositorInsertDmCYFIP1 (Sra-1 Fly)
UseTagsExpressionInsectMutation5 silent and one non silent A146V mutations compa…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmCYFIP1_T
Plasmid#147585PurposeInsect Expression of DmCYFIP1DepositorInsertDmCYFIP1 (Sra-1 Fly)
UseTagsExpressionInsectMutation5 silent and one non silent A146V mutations compa…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmCYFIP1-4xmut_T
Plasmid#147586PurposeInsect Expression of DmCYFIP1-4xmutDepositorInsertDmCYFIP1-4xmut (Sra-1 Fly)
UseTagsExpressionInsectMutation5 silent and one non silent A146V mutations compa…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmCYFIP1-K743E_T
Plasmid#147587PurposeInsect Expression of DmCYFIP1-K743EDepositorInsertDmCYFIP1-K743E (Sra-1 Fly)
UseTagsExpressionInsectMutation5 silent and one non silent A146V mutations compa…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKIF1C_P176L-mCherry-FRB
Plasmid#185983PurposeMammalian expression of human KIF1C with P176L mutation and mCherry and FRB tags.DepositorInsertKIF1C (KIF1C Human)
UseTagsmCherry and FRBExpressionMammalianMutationP176L - mutation causing HSP (SPG58)PromoterAvailable sinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKIF1C_R169W-mCherry-FRB
Plasmid#185984PurposeMammalian expression of human KIF1C with R169W mutation and mCherry and FRB tags.DepositorInsertKIF1C (KIF1C Human)
UseTagsmCherry and FRBExpressionMammalianMutationR169W - mutation causing HSP (SPG58) and silent m…PromoterAvailable sinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQlinkG2-PTPIP51(236-470)
Plasmid#170534PurposeExpresses Cleavable GST-tagged PTPIP51 TPR domain (236-470) in E. ColiDepositorInsertProtein tyrosine phosphatase interacting protein 51 (RMDN3 Human)
UseTagsGST tagExpressionBacterialMutationPromoterT5 promoterAvailable sinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQlinkH-PTPIP51(236-470)
Plasmid#170530PurposeExpresses Cleavable His-tagged PTPIP51 TPR domain (236-470) in E. ColiDepositorInsertProtein tyrosine phosphatase interacting protein 51 (236-470) (RMDN3 Human)
UseTagsHis tagExpressionBacterialMutationPromoterT5 promoterAvailable sinceJuly 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pWPXL-p150-PIP1Δ
Plasmid#160993PurposeExpress mouse p150 protein with PIP1 deletion mutationDepositorInsertCHAF1A (Chaf1a Mouse)
UseTagsGFPExpressionMammalianMutationPIP1 (PCNA-interacting protein) motif deletionPromoterAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTAMAHISTEV_Ab-PirA
Plasmid#128959Purposeexpresses Acinetobacter baumannii PirA in E. coliDepositorInsertferric enterobactin receptor PirA
UseTagsTEV protease cleavable 7xHis and TamAExpressionBacterialMutationSignal peptide sequence has been removedPromoterT7Available sinceAug. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNAS1b split mCherry NEDD4 WW2 neg ctrl
Plasmid#112030PurposeMode #2 phosphosite negative control (split mCherry, using NEDD4 WW2 binding domain paired with negative control peptide)DepositorInsertNEDD4 WW2 domain fused to C-terminal split mCherry, neg ctrl interacting peptide fused to N-terminal split mCherry
UseTagsSplit mCherryExpressionBacterialMutationPromoterAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19-E6KTN8
Plasmid#103076PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertE6KTN8(Cas9 coding gene from Actinomyces sp. oral taxon 180 str. F0310)
UseCRISPR; Cloning vectorTagsExpressionMutationhuman codon-optimizedPromoterAvailable sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcdna3 T7 Jip1b(471-707)(C478S and C699T)
Plasmid#52132Purposea mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.DepositorInsertJNK interacting protein-1b (JIP-1b)(471-707)(M1+6 ) (Mapk8ip1 Mouse)
UseTagsT7ExpressionMammalianMutationdeleted amino acid 1-470 and changed C478S and C6…PromoterAvailable sinceJune 11, 2014AvailabilityAcademic Institutions and Nonprofits only