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Showing: 2921 - 2940 of 5031 results

  4. pABD1031

    Plasmid
    #68004
    Purpose
    Protein co-localization of PIF1 (PIL5) via fluorescent fusion after transient expression in planta
    Depositor
    Bruce Downie
    Article
    Majee et al Proc Natl Acad Sci U S A. 2018 Apr 24;11
    Insert
    PHY-INTERACTING FACTOR 1 (PIF1) (PIL5 )
    Use
    Tags
    eYFP
    Expression
    Plant
    Mutation
    Promoter
    2X Cauliflower Mosaic Virus 35S promoter
    Available since
    Dec. 17, 2018
    Availability
    Academic Institutions and Nonprofits only

  5. pABD1275

    Plasmid
    #68017
    Purpose
    PIF1 protein overproduction for purification from bacterial cells
    Depositor
    Bruce Downie
    Article
    Majee et al Proc Natl Acad Sci U S A. 2018 Apr 24;11
    Insert
    PHY-INTERACTING FACTOR 1 (PIF1) (PIL5 AT2G20180, Mustard Weed)
    Use
    Tags
    6x-His
    Expression
    Bacterial
    Mutation
    Codon Optimized for bacterial expression (GenScri…
    Promoter
    T7
    Available since
    Dec. 17, 2018
    Availability
    Academic Institutions and Nonprofits only

  8. pBa-LSS-GFP-LDLR wt

    Plasmid
    #98184
    Purpose
    Low Density Lipoprotein Receptor N-terminally tagged with Green Fluorescent Protein. LSS= LDLR signal sequence, which is cleaved leaving the GFP attached to the mature LDLR protien.
    Depositor
    Gary Banker, Marvin Bentley
    Article
    Jenkins et al J Cell Biol. 2012 Aug 20;198(4):749-61.
    Insert
    LDLR (LDLR Human)
    Use
    Tags
    GFP and LDLR signal sequence, N-term of GFP so GF…
    Expression
    Mammalian
    Mutation
    none
    Promoter
    Chicken Beta Actin
    Available since
    Sept. 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    98184-plasmid-map-sequence-id-193286

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  9. WT Smarca4-sfGFP

    Plasmid
    #107056
    Purpose
    Expression of wild-type Smarca4 (Brg1) with C-terminal super-folder GFP fusion.
    Depositor
    Courtney Hodges, Jerry Crabtree
    Article
    Hodges et al Nat Struct Mol Biol. 2018 Jan;25(1):61-7
    Insert
    SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (Smarca4 Mouse)
    Use
    Lentiviral
    Tags
    Super-folder GFP (sfGFP)
    Expression
    Mammalian
    Mutation
    wild-type
    Promoter
    CAG
    Available since
    April 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    107056-plasmid-map-sequence-id-321500

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  10. WT Smarca4-V5 IRES-puro

    Plasmid
    #107058
    Purpose
    Expression of wild-type Smarca4 (Brg1)-V5 fusion with IRES puromycin resistance.
    Depositor
    Courtney Hodges, Jerry Crabtree
    Article
    Hodges et al Nat Struct Mol Biol. 2018 Jan;25(1):61-7
    Insert
    SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (Smarca4 Mouse)
    Use
    Lentiviral
    Tags
    IRES-puroR and V5
    Expression
    Mammalian
    Mutation
    wild-type
    Promoter
    CAG
    Available since
    April 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    107058-plasmid-map-sequence-id-201960

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  11. pHLmMBP-10

    Plasmid
    #72348
    Purpose
    Mammalian expression of secreted N-terminally 8His-tagged mMBP TEV cleavage site-linked fusion proteins with C-terminal 6His-tag/Strep-tag II/HA-tag
    Depositor
    Luca Jovine
    Article
    Bokhove et al J Struct Biol. 2016 Feb 3. 194:1-7. pii
    Type
    Empty backbone
    Use
    Tags
    6His-tag, 8His-tag, HA-tag, Strep-tag II, and mMB…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV enhancer + chicken beta-actin promoter
    Available since
    Feb. 17, 2016
    Availability
    Academic Institutions and Nonprofits only
    72348-plasmid-map-sequence-id-133757

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  12. pAAV hPGRN

    Plasmid
    #213682
    Purpose
    Expresses human progranulin (PGRN) with an N-terminal twin-Strep-V5 tag
    Depositor
    Thomas Kukar
    Article
    Root et al Cell Rep. 2024 Nov 19;43(12):114985. doi
    Insert
    Human Progranulin (hPGRN) (GRN Human)
    Use
    AAV
    Tags
    Twin-Strep tag and V5 tag (after signal peptide)
    Expression
    Mutation
    Promoter
    cytomegalovirus enhancer/chicken β-actin promoter
    Available since
    Dec. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    213682-plasmid-map-sequence-id-455751

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  13. K785R Smarca4-sfGFP

    Plasmid
    #107057
    Purpose
    Expression of dominant-negative K785R Smarca4 (Brg1) with C-terminal super-folder GFP fusion.
    Depositor
    Courtney Hodges, Jerry Crabtree
    Article
    Hodges et al Nat Struct Mol Biol. 2018 Jan;25(1):61-7
    Insert
    SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (Smarca4 Mouse)
    Use
    Lentiviral
    Tags
    Super-folder GFP (sfGFP)
    Expression
    Mammalian
    Mutation
    K785R
    Promoter
    CAG
    Available since
    April 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    107057-plasmid-map-sequence-id-201959

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  15. qTAG-N-Puro-mStayGold-ACTB

    Plasmid
    #227320
    Purpose
    Donor template for Puro-2A-mStayGold insertion into the N-terminus of the ACTB locus. For actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB (Addgene #207748)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    ACTB Homology Arms flanking a Puro-2A-mStayGold Cassette (ACTB Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Promoterless
    Available since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227320-plasmid-map-sequence-id-452091

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  18. pACT1:Cas9-GFP, U6:sgUPRT

    Plasmid
    #122853
    Purpose
    Expresses Cas9 fused with GFP and a sgRNA targeting the Cryptosporidium parvum UPRT gene
    Depositor
    David Sibley
    Article
    Wilke et al Cell Host Microbe. 2019 Jun 18. pii: S19
    Inserts
    Cas9-GFP
    U6-sgUPRT
    Use
    CRISPR; Cas9-gfp plasmid for genome editing of cr…
    Tags
    eGFP
    Expression
    Mutation
    Promoter
    Cryptosporidium parvum U6 and Cryptosporidium par…
    Available since
    July 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    122853-plasmid-map-sequence-id-238877

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  19. K785R Smarca4-V5 IRES-puro

    Plasmid
    #107059
    Purpose
    Expression of dominant-negative K785R Smarca4 (Brg1)-V5 fusion with IRES puromycin resistance.
    Depositor
    Courtney Hodges, Jerry Crabtree
    Article
    Hodges et al Nat Struct Mol Biol. 2018 Jan;25(1):61-7
    Insert
    SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (Smarca4 Mouse)
    Use
    Lentiviral
    Tags
    IRES-puroR and V5
    Expression
    Mammalian
    Mutation
    K785R
    Promoter
    CAG
    Available since
    April 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    107059-plasmid-map-sequence-id-201961

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  20. pACT1:Cas9-GFP, U6:sgTK

    Plasmid
    #122852
    Purpose
    Expresses Cas9 fused with GFP and a sgRNA targeting the Cryptosporidium parvum TK gene
    Depositor
    David Sibley
    Article
    Wilke et al Cell Host Microbe. 2019 Jun 18. pii: S19
    Inserts
    Cas9-GFP
    U6-sgTK
    Use
    CRISPR; Cas9-gfp plasmid for genome editing of cr…
    Tags
    eGFP
    Expression
    Mutation
    Promoter
    Cryptosporidium parvum U6 and Cryptosporidium par…
    Available since
    July 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    122852-plasmid-map-sequence-id-238880

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Showing: 2921 - 2940 of 5031 results