Addgene

We narrowed to 4,432 results for: ttl

Showing: 2901 - 2920 of 4432 results

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pBS4S

Plasmid

#55170

Purpose

Empty vector, integration at thrC, ampr, specr

Depositor

Thorsten Mascher

Article

Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

Type

Empty backbone

Use

Synthetic Biology; Bacillus biobrick box

Tags

Expression

Mutation

Promoter

none

Available since

Aug. 1, 2014

Availability

Academic Institutions and Nonprofits only
pdCASclos

Plasmid

#73640

Purpose

Transcriptional repression for gene Spo0A (CAC-2011) in Clostridium acetobutylicum ATCC 824

Depositor

Sheng Yang

Article

Li et al Biotechnol J. 2016 May 23. doi: 10.1002/

Inserts

dCas9
sgRNA to spo0A

Use

E.coli-clostridium shuttle vector

Tags

Expression

Mutation

D10A, H840A

Promoter

Pj23119 and ptb

Available since

June 22, 2016

Availability

Academic Institutions and Nonprofits only

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pOCC72

Plasmid

#118866

Purpose

shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and N-terminal monomeric GFP, cleavable with TEV

Depositor

Marc Bickle, PEPC MPI CBG

Article

Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

Insert

NcoI-HIS6-3C-mGFP-TEV-NotI-ccdB-AscI-stop-HindIII cassette

Use

Tags

HIS6, cleavable with 3C protease, mGFP, cleavable…

Expression

Insect

Mutation

Promoter

polH

Available since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pOCC28

Plasmid

#118873

Purpose

shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal MBP tag, cleavable with 3C, and C-terminal HIS6 tag, cleavable with 3C

Depositor

Marc Bickle, PEPC MPI CBG

Article

Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

Insert

NcoI-MBP-3C--NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette

Use

Tags

HIS6, cleavable with 3C protease and MBP, cleavab…

Expression

Insect

Mutation

Promoter

polH

Available since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pOCC102

Plasmid

#118883

Purpose

shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C protease

Depositor

Marc Bickle, PEPC MPI CBG

Article

Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

Insert

NcoI-HIS6-MBP-3C-NotI-ccdB-AscI-stop-HindIII cassette

Use

Tags

HIS6-MBP, cleavable with 3C protease

Expression

Insect

Mutation

Promoter

polH

Available since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pRAN473

Plasmid

#120813

Purpose

E. coli-C. difficile shuttle vector: tetracycline-inducible promoter (Ptet) driving expression of red fluorescent protein (mCherryOpt), multiple cloning site for fusion to N-terminus of target protein

Depositor

Craig Ellermeier, David S. Weiss

Article

Ransom et al Appl Environ Microbiol. 2015 Mar;81(5):1

Insert

red fluorescent protein (mCherry), codon-optimized for C. difficile, multiple-cloning site for protein fusion

Use

Tags

Expression

Bacterial

Mutation

Promoter

Ptet

Available since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pOCC167

Plasmid

#118909

Purpose

shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with honey bee melitine secretion signal and C-terminal HIS6, cleavable with 3C protease

Depositor

Marc Bickle, PEPC MPI CBG

Article

Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

Insert

NcoI-HBMss-NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette

Use

Tags

HIS6, cleavable with 3C protease

Expression

Insect

Mutation

Promoter

polH

Available since

Jan. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR221-PGK.Pro

Plasmid

#79489

Purpose

MultiSite Gateway entry clones, first fragment, (attL1, attR5) Human PGK promoter

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

PGK Promoter (PGK1 Synthetic, Human)

Use

Multisite gateway entry clone

Tags

Expression

Mutation

Promoter

PGK

Available since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

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pOCC267

Plasmid

#118887

Purpose

shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and N-terminal HALO tag

Depositor

Marc Bickle, PEPC MPI CBG

Article

Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

Insert

NcoI-HIS6-MBP-3C-HALO-NotI-ccdB-AscI-stop-HindIII cassette

Use

Tags

HIS6-MBP, cleavable with 3C protease; HALO tag

Expression

Insect

Mutation

Promoter

polH

Available since

Jan. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pHCas9-Nours

Plasmid

#107733

Purpose

Escherichia coli- S. cerevisiae shuttle plasmid harbors a Cas9 gene, a natMX6 and URA3 selection markers used in S. cerevisiae

Depositor

Sheng Yang

Article

Wang et al Biotechnol Bioeng. 2018 Sep 10. doi: 10.

Insert

Cas9

Use

CRISPR

Tags

SV40 NLS

Expression

Yeast

Mutation

Human optimized

Promoter

TEF1

Available since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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Ad-CBR (GFP/cAPC)

Plasmid

#16576

Depositor

Bert Vogelstein

Article

Shih et al Cancer Res. 2000 Mar 15. 60(6):1671-6.

Insert

cAPC (APC Human)

Use

Adenoviral

Tags

EGFP

Expression

Mammalian

Mutation

Residues 958-2076

Promoter

Available since

July 3, 2008

Availability

Academic Institutions and Nonprofits only
gCOTS-pyl

Plasmid

#92048

Purpose

This is a S. elongatus (PCC7942) shuttle vector used for recombination of the pylRS orthogonal translation system to genomic neutral site 2.

Depositor

Lital Alfonta

Article

Chemla et al Biochemistry. 2017 Apr 12. doi: 10.1021/

Inserts

Pyrrolysyl tRNA(cua)
Pyrrolysyl tRNA synthetase (methanosarcina mazei)

Use

Cyanobacteria s. elongatus genome recombination i…

Tags

Expression

Mutation

Promoter

LeuP (native S. elongatus promoter) and PrcbL

Available since

Aug. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pOEM-GED-EGFP

Plasmid

#102782

Purpose

Baculovirus rescue vector, VSVGED pseudotype, eGFP

Depositor

Elly Tanaka

Article

Oliveira et al Dev Biol. 2018 Jan 15;433(2):262-275. do

Insert

EGFP

Use

Baculoviral rescue shuttle vector

Tags

Expression

Insect and Mammalian

Mutation

Promoter

CMV

Available since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pOCC193

Plasmid

#118903

Purpose

shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal MBP, cleavable with 3C and monomeric GFP and C-terminal TwinStrep, cleavable with 3C

Depositor

Marc Bickle, PEPC MPI CBG

Article

Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

Insert

NcoI-MBP-3C-mGFP-NotI-ccdB-AscI-3C-TwinStrep-stop-HindIII cassette

Use

Tags

MBP, cleavable with 3C protease; mGFP and TwinStr…

Expression

Insect

Mutation

Promoter

polH

Available since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pOCC305

Plasmid

#118898

Purpose

shuttle vector for baculovirus production, using FlashBac bacmid; for rec. protein with N-terminal TwinStrep-MBP tag, cleavable with 3C and C-terminal HIS6, cleavable with 3C

Depositor

Marc Bickle, PEPC MPI CBG

Article

Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

Insert

NcoI-TwinStrep-MBP-3C-NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette

Use

Tags

HIS6, cleavable with 3C protease and TwinStrep-MB…

Expression

Insect

Mutation

Promoter

polH

Available since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pOCC93

Plasmid

#118911

Purpose

shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with GP64 viral secretion signal and C-terminal eGFP-HIS6

Depositor

Marc Bickle, PEPC MPI CBG

Article

Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

Insert

NcoI-GP64ss-NotI-ccdB-AscI-eGFP-HIS6-stop-HindIII cassette

Use

Tags

eGFP-HIS6

Expression

Insect

Mutation

Promoter

polH

Available since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pBEG R2-iCreT2ALuc-L3 #BGV208

Plasmid

#48992

Purpose

Supplies Cre recombinase and Luciferase and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid).

Depositor

David Dankort

Article

Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

Inserts

cre recombinase
Luciferase

Use

Luciferase; Gateway cloning vector

Tags

IRES (weak)

Expression

Mutation

Promoter

Unknown

Available since

June 16, 2014

Availability

Academic Institutions and Nonprofits only
pOCC121

Plasmid

#118893

Purpose

shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal MBP tag, cleavable with 3C, and monomeric GFP and C-terminal HIS6, cleavable with 3C

Depositor

Marc Bickle, PEPC MPI CBG

Article

Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

Insert

NcoI-MBP-3C-mGFP-NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette

Use

Tags

HIS6, cleavable with 3C protease and MBP, cleavab…

Expression

Insect

Mutation

Promoter

polH

Available since

Jan. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pCEP4-SERTS585P-myc

Plasmid

#107498

Purpose

Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation S585P conferring gain of function phenotype

Depositor

Erik Procko

Article

Young et al bioRxiv 2021.04.19.440442

Insert

Serotonin Transporter (SERT) (SLC6A4 Human)

Use

Tags

Expression

Mammalian

Mutation

S585P

Promoter

CMV

Available since

March 15, 2018

Availability

Academic Institutions and Nonprofits only

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pOCC70

Plasmid

#118878

Purpose

shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal monomeric GFP, cleavable with TEV, and C-terminal HIS6 tag, cleavable with 3C

Depositor

Marc Bickle, PEPC MPI CBG

Article

Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

Insert

NcoI-NotI-ccdB-AscI-TEV-mGFP-3C-HIS6-stop-HindIII cassette

Use

Tags

mGFP, cleavable with TEV protease; HIS6, cleavabl…

Expression

Insect

Mutation

Promoter

polH

Available since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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