Addgene

We narrowed to 5,034 results for: pcdna3.1

Showing: 2901 - 2920 of 5034 results

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pNIR-FbALFA

Plasmid

#184678

Purpose

NIR-Fb to ALFA-tag

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

Insert

NIR-FbALFA

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 23, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-DN-hCUL3-FLAG

Plasmid

#15820

Depositor

Wade Harper

Article

Jin et al Methods Enzymol. 2005 . 399():287-309.

Insert

Cullin3 (CUL3 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Dominant negative: N418

Promoter

Available since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

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Mito-mhYFP

Plasmid

#186526

Purpose

Localization of monomeric hyperfolder YFP fluorescent protein to mitochondria

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

Monomeric hyperfolder YFP

Use

Tags

Human COX8a first 29 amino acids

Expression

Mammalian

Mutation

hfYFP-S147P/L195M/V206K

Promoter

CMV

Available since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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4xHRE_minCMV-sfGFP-CMV_dsRed Exp

Plasmid

#117401

Purpose

sfGFP expressed from hypoxia-inducible minimal CMV promoter and dsRed expressed from consitutive CMV promoter

Depositor

Yvonne Chen

Article

Ede et al ACS Synth Biol. 2016 May 20;5(5):395-404

Insert

SuperFolder GFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 24, 2018

Availability

Academic Institutions and Nonprofits only

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VinculinTS-A50I/I997A

Plasmid

#111829

Purpose

Vinculin tension sensor with point mutations affecting ability to bind talin and actin

Depositor

Brenton Hoffman

Article

Rothenberg et al Biophys J. 2018 Apr 10;114(7):1680-1694.

Insert

VinculinTS (I997A)

Use

Tags

Expression

Mammalian

Mutation

changed vinculin alanine 50 to isoleucine and iso…

Promoter

CMV

Available since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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Fra-2

Plasmid

#187907

Purpose

Expresses Fra-2 in transfected cells

Depositor

Jennifer Mitchell

Article

Mitchell et al Endocrinology. 2005 Apr;146(4):2048-54.

Insert

Fra2 (Fosl2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

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mNeonGreen-DEVD-NanoLuc

Plasmid

#98287

Purpose

caspase-3 BRET Sensor

Depositor

Maarten Merkx

Article

den Hamer et al ACS Sens. 2017 Jun 23;2(6):729-734. doi:

Insert

mNeonGreen-LEHD-NanoLuc

Use

Tags

His-tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 11, 2019

Availability

Academic Institutions and Nonprofits only

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pFH3.29_Cre

Plasmid

#205560

Purpose

Express Cre (without export tag)

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

Cre

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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ZipGFP1-10_TEV

Plasmid

#81242

Purpose

Zipped GFP1-10 with TEV cleavage sequence T2A mCherry

Depositor

Xiaokun Shu

Article

To et al Cell Chem Biol. 2016 Jul 21;23(7):875-82

Insert

zipped GFP11 with TEV cleavage sequence T2A mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 18, 2016

Availability

Academic Institutions and Nonprofits only

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RET-WT

Plasmid

#125252

Purpose

Expresses human RET in mammalian cells

Depositor

Sissy Jhiang

Article

Xing et al Endocrinology. 1996 May;137(5):1512-9. d

Insert

RET (RET Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-DN-hCUL4B-FLAG

Plasmid

#15822

Depositor

Wade Harper

Article

Jin et al Methods Enzymol. 2005 . 399():287-309.

Insert

Cullin4B (CUL4B Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Dominant negative: N594

Promoter

Available since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA-Flag-Kdm4b-pA

Plasmid

#104486

Purpose

Express mouse Kdm4b in mammalian cells. It has a T7 promoter for in vitro transcription. Since polyA is encoded on the vector, polyA-tailing is unnecessary.

Depositor

Yi Zhang

Article

Inoue et al Genes Dev. 2017 Oct 1;31(19):1927-1932.

Insert

Kdm4b (Kdm4b Mouse)

Use

Tags

Flag epitope tag

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 19, 2017

Availability

Academic Institutions and Nonprofits only

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mNeonGreen-IETD-NanoLuc

Plasmid

#98288

Purpose

caspase-8 BRET Sensor

Depositor

Maarten Merkx

Article

den Hamer et al ACS Sens. 2017 Jun 23;2(6):729-734. doi:

Insert

mNeonGreen-LEHD-NanoLuc

Use

Tags

His-tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 11, 2019

Availability

Academic Institutions and Nonprofits only

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Fra-1

Plasmid

#187906

Purpose

Expresses Fra-1 in transfected cells

Depositor

Jennifer Mitchell

Article

Mitchell et al Endocrinology. 2005 Apr;146(4):2048-54.

Insert

Fra1 (Fosl1 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA3-mitoT

Plasmid

#174519

Purpose

Engineered intermembrane bridge to link the inner and outer mitochondrial membranes in mammalian cells

Depositor

Oleh Khalimonchuk

Article

Viana et al iScience. 2021 Jan 29;24(2):102119. doi:

Insert

MitoT synthetic tether

Use

Tags

6xHis and eGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 16, 2021

Availability

Academic Institutions and Nonprofits only

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Mammalian_SpCas9_Target-AID3S

Plasmid

#188649

Purpose

Expresses SpCas9_Target-AID3S in mammalian cells

Depositor

Keiji Nishida

Article

Li et al Nat Commun. 2022 Aug 8;13(1):4531. doi:

Insert

nCas9-AID3S-UGI

Use

Tags

UGI

Expression

Mammalian

Mutation

D10A for SpCas9

Promoter

pCMV

Available since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pVH233-Tier1-PmPGK1-eYFP

Plasmid

#169522

Purpose

Tier-1 vector encoding PmPGK1-driven eYFP expression (PmPGK1-eYFP-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PPGK-driven enhanced yellow fluorescent protein

Use

Tags

Expression

Mammalian

Mutation

Promoter

PPGK

Available since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pNbGFP-miRFP670nano3

Plasmid

#184687

Purpose

Nanobody to GFP labelled with near-infrared fluorescent protein miRFP670nano3

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

Insert

NbGFP-miRFP670nano3

Use

Tags

miRFP670nano3

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 23, 2022

Availability

Academic Institutions and Nonprofits only

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4xHRE_YB TATA-sfGFP-CMV_dsRed

Plasmid

#117399

Purpose

sfGFP expressed from hypoxia-inducible YB_TATA promoter and dsRed expressed from consitutive CMV promoter

Depositor

Yvonne Chen

Article

Ede et al ACS Synth Biol. 2016 May 20;5(5):395-404

Insert

SuperFolder GFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 24, 2018

Availability

Academic Institutions and Nonprofits only

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ER2-mhYFP

Plasmid

#186528

Purpose

Localization and retention of monomeric hyperfolder YFP fluorescent protein in the ER; improved targeting efficiency

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

Monomeric hyperfolder YFP

Use

Tags

Calreticulin and KDEL

Expression

Mammalian

Mutation

hfYFP-S147P/L195M/V206K

Promoter

CMV

Available since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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