Addgene

We narrowed to 11,285 results for: pcDNA

Showing: 2901 - 2920 of 11285 results

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pcDNA5-FRT-TO-Nsp1-HF

Plasmid

#157681

Purpose

mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp1 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

HF-Nsp1 (ORF1ab SARS-CoV-2)

Use

Tags

His8-Flag

Expression

Mammalian

Mutation

codon optimized

Promoter

Available since

Aug. 4, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3.1/HisC 14-3-3 sigma

Plasmid

#11947

Depositor

Michael Yaffe

Article

Wilker et al J Biol Chem. 2005 May 13. 280(19):18891-

Insert

Stratifin (SFN Human)

Use

Tags

His

Expression

Mammalian

Mutation

Promoter

Available since

May 17, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA4-TO-Myc-rZAP

Plasmid

#17381

Depositor

Stephen Goff

Article

Gao et al Science. 2002 Sep 6. 297(5587):1703-6.

Insert

ZAP (Zc3hav1 Rat)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

March 1, 2010

Availability

Academic Institutions and Nonprofits only
pY126 (pcDNA3.1-huBoCpf1)

Plasmid

#92302

Purpose

Expression of humanized BoCpf1

Depositor

Feng Zhang

Insert

huBoCpf1

Use

Tags

NLS and NLS-3xHA

Expression

Mammalian

Mutation

Promoter

Available since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

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pY113 (pcDNA3.1-huPb2Cpf1)

Plasmid

#92272

Purpose

Expression of humanized Pb2Cpf1

Depositor

Feng Zhang

Insert

huPb2Cpf1

Use

Tags

NLS and NLS-3xHA

Expression

Mammalian

Mutation

Promoter

Available since

June 22, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/TO-UBE2QL1-Strep/HA

Plasmid

#124666

Purpose

Expresses UBE2QL1 with C-terminal Strep-HA tag in mammalian cells

Depositor

Hemmo Meyer

Article

Koerver et al EMBO Rep. 2019 Aug 21:e48014. doi: 10.15

Insert

UBE2QL1 (UBE2QL1 Human)

Use

Tags

2xStrep/HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag MKK7g1

Plasmid

#14624

Depositor

Roger Davis

Article

Tournier et al Mol Cell Biol. 1999 Feb . 19(2):1569-81.

Insert

MKK7g1 (Map2k7 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

July 25, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA-GFP-BIRC3-LU

Plasmid

#160700

Purpose

Expresses GFP-BIRC3 with the long 3'UTR in mammalian cells

Depositor

Christine Mayr

Article

Lee et al Mol Cell. 2019 May 16;74(4):701-712.e9.

Insert

GFP-BIRC3 (BIRC3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 26, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Gαi*-BERKY1

Plasmid

#158090

Purpose

BRET biosensor for detection of Gαi-GTP in mammalian expression vector pcDNA3.1(+)

Depositor

Mikel Garcia-Marcos

Article

Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

Insert

Lyn11-Nluc-ER/K linker-YFP-KB1753-myc

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-sACE2v2-Fc(IgA1)

Plasmid

#145157

Purpose

Mammalian expression plasmid for soluble ACE2-Fc fusion (IgA1) high affinity variant 2

Depositor

Erik Procko

Article

Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

Insert

Angiotensin-converting enzyme 2 (ACE2 Human)

Use

Tags

Fc region of human IgA1

Expression

Mammalian

Mutation

Extracellular, soluble protease domain (a.a. M1-D…

Promoter

CMV

Available since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Gαi*-BERKY2

Plasmid

#158197

Purpose

BRET biosensor for detection of Gαi-GTP in mammalian expression vector pcDNA3.1(+)

Depositor

Mikel Garcia-Marcos

Article

Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

Insert

Lyn11-Nluc-ER/K linker-2xYFP-KB1753-myc

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-tRSA-G quartet

Plasmid

#32203

Depositor

Ian Macara

Article

Iioka et al Nucleic Acids Res. 2011 Apr;39(8):e53. E

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only

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Y771F EphA2 pcDNA3

Plasmid

#102735

Purpose

Expression of EphA2 in mammalian cells

Depositor

Jin Chen

Article

Fang et al J Biol Chem. 2008 Jun 6;283(23):16017-26

Insert

EphA2 (Epha2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Y771F (TAC > TTC)

Promoter

CMV

Available since

Nov. 8, 2017

Availability

Academic Institutions and Nonprofits only

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Y593E EphA2 pcDNA3

Plasmid

#102731

Purpose

Expression of EphA2 in mammalian cells

Depositor

Jin Chen

Article

Fang et al J Biol Chem. 2008 Jun 6;283(23):16017-26

Insert

EphA2 (Epha2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Y593E (TAC > GAG)

Promoter

CMV

Available since

Nov. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA3/FKBP12-pBirA(79-C)-ha

Plasmid

#154449

Purpose

expresses FKBP12-pBirA(79-C)-ha/biochemical assay

Depositor

Hyun-Woo Rhee

Article

Kwak et al Proc Natl Acad Sci U S A. 2020 May 15. p

Insert

FKBP12-pBirA(79-C)-ha (FKBP12 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

T7 promoter

Available since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA3.0_LynSNAP IRES EGFP

Plasmid

#136625

Purpose

Plasmid encoding outer inner membrane localization of SNAP-Tag and EGFP

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

Lyn11-SNAP-Tag (IRES EGFP)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-hMcl-1(S159A)

Plasmid

#25374

Depositor

Roger Davis

Article

Morel et al Mol Cell Biol. 2009 Jul . 29(14):3845-52

Insert

hMCL1(S159A) (MCL1 Human)

Use

Tags

V5 and His tag

Expression

Mammalian

Mutation

changed serine 159 to alanine

Promoter

Available since

Aug. 23, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA4-FKBP-LAP1

Plasmid

#34841

Depositor

Alice Ting

Article

Slavoff et al J Am Chem Soc. 2011 Dec 14;133(49):19769

Insert

FKBP-LAP1

Use

Tags

His Tag, T7 tag, and myc

Expression

Mutation

Promoter

CMV

Available since

Feb. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3-CTE-Luc

Plasmid

#60881

Purpose

Reporter construct to study unspliced RNA export. Coding sequence of luciferase gene was sandwiched between 5' and 3' splice site sequences originating from the HIV gag/pol gene followed by CTE

Depositor

Vinay Nandicoori

Article

Rajanala et al PLoS One. 2012;7(1):e29921. doi: 10.1371

Insert

Luc-CTE

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO DNAJA3

Plasmid

#19466

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

Insert

DNAJA3 (DNAJA3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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