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Plasmid#111946PurposeThis is a shuffle plasmid containing the HSH155 gene and the surrounding genomic regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has the Q773A R775A R776A Q778A mutationDepositorInsertHsh155 Q773A R775A R776A Q778A (C4A)
UseTagsExpressionBacterial and YeastMutationQ773A R775A R776A Q778APromoterAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
MR015-RBM20 Left TALEN
Plasmid#108342PurposeLeft TALEN for RBM20 with NNDepositorInsertTALEN
UseTALENTagsExpressionMammalianMutationPromoterAvailable sinceJune 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
MR015-RBM20 Right TALEN
Plasmid#108343PurposeRight TALEN for RBM20 with NNDepositorInsertTALEN
UseTALENTagsExpressionMammalianMutationPromoterAvailable sinceJune 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTRE/SP M87 N184X
Plasmid#92373PurposeInducible expression of M87 N184X spastin in mammalian cellsDepositorInsertspastin (SPAST Human)
UseTagsExpressionMammalianMutation1-460 bp deleted, N184XPromoterTet-responsive P tightAvailable sinceJune 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTRE/SP S245X with 5'UTR
Plasmid#92376PurposeInducible expression of S245X spastin M1 and M87 in mammalian cellsDepositorInsertspastin (SPAST Human)
UseTagsExpressionMammalianMutationS245XPromoterTet-responsive P tightAvailable sinceJune 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTRE/SP M87 S245X
Plasmid#92374PurposeInducible expression of M87 S245X spastin in mammalian cellsDepositorInsertspastin (SPAST Human)
UseTagsExpressionMammalianMutation1-460 bp deleted, S245XPromoterTet-responsive P tightAvailable sinceJune 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag/SP S245X with 5'UTR
Plasmid#92370Purposeexpression of 245X spastin M1 and M87 in mammalian cellsDepositorInsertspastin (SPAST Human)
UseTagsExpressionMammalianMutationS245XPromoterCMVAvailable sinceJune 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag/M87 N184X
Plasmid#92367Purposeexpression of M87 N184X spastin in mammalian cellsDepositorInsertspastin (SPAST Human)
UseTagsExpressionMammalianMutation1-460 bp deleted, N184XPromoterCMVAvailable sinceJune 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag/M1 N184X
Plasmid#92365Purposeexpression of M1 N184X spastin in mammalian cellsDepositorInsertspastin (SPAST Human)
UseTagsExpressionMammalianMutation1-194 bp of 5'UTR deleted, N184XPromoterCMVAvailable sinceJune 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFosill-4
Plasmid#89698Purposeto make Fosmids that can be converted into Illumina sequencable librariesDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMay 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
isoform PKC Eta (JCE615)
Plasmid#89774Purposeyeast expression of mouse PKC Eta with yeast PKC1 promoter and ADH1 terminatorDepositorInsertPKC Eta (Prkch Mouse)
UseTagsExpressionMammalianMutationPromoteryeast PKC1 promoter (-600 to -1)Available sinceApril 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCALNL_Ch5_155183064-155183141
Plasmid#81211PurposepCALNL reporter contains two target sites consisting of PAM Cas9 site-gix psuedo site-Cas9 site-PAM that match region in centromeres of chromosomes 5 (numbers in common name refer to pos. in hg38)DepositorInsertgix-Neo-gix deletion cassette upstream of EGFP
UseTagsExpressionMammalianMutationPromoterpCBaAvailable sinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pmcsg7-mCRT(D311A)
Plasmid#83509PurposeBacterial expression of Calreticulin mutantDepositorInsertCALR (Calr Mouse)
UseTagsHisExpressionBacterialMutationD311APromoterT7Available sinceOct. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pmcsg7-mCRT(1-351)
Plasmid#83508PurposeBacterial expression of Calreticulin mutantDepositorInsertCALR (Calr Mouse)
UseTagsHisExpressionBacterialMutation1-351 onlyPromoterT7Available sinceOct. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
shCDK4(1)
Plasmid#73550PurposeshRNA(1) against CDK4DepositorInsertshRNA against CDK4
UseRetroviralTagsExpressionMammalianMutationPromoterLTRAvailable sinceAug. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
shCDK4(2)
Plasmid#73551PurposeshRNA(2) against CDK4DepositorInsertshRNA against CDK4
UseRetroviralTagsExpressionMammalianMutationPromoterLTRAvailable sinceAug. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestFLAGHA_RBPMS_R38E
Plasmid#65665PurposeFLAG-HA expression vector with RBPMS carrying mutation R38EDepositorInsertRBPMS (RBPMS Human)
UseTagsFLAG HAExpressionMammalianMutationchanged arginine 38 to glutamic acidPromoterCMV, 2x TetAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestFLAGHA_RBPMS_R38Q
Plasmid#65094PurposeDestination vector with RBPMS with R38Q mutation for stable cell line generationDepositorInsertRBPMS (RBPMS Human)
UseTagsFLAG HAExpressionMammalianMutationchanged arginine 38 to glutaminePromoterCMV, 2x TetAvailable sinceJune 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestFLAGHA_RBPMS_R38A_E39A
Plasmid#65664PurposeFLAG-HA expression vector with RBPMS carrying mutation R38A, E39ADepositorInsertRBPMS (RBPMS Human)
UseTagsFLAG HAExpressionMammalianMutationchanged arginine 38 to alanine, and glutamic acid…PromoterCMV, 2x TetAvailable sinceJune 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCVD403
Plasmid#63740PurposeContains the LT gene probe (HincII fragment) cloned on BamHI linkers into pACYC184DepositorInsertLT gene probe
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 6, 2015AvailabilityAcademic Institutions and Nonprofits only