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We narrowed to 29,293 results for: uros

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MSCV-EZH2_EZH1SET-PGK-Puro-IRES-GFP

Plasmid

#75126

Purpose

Retroviral expression plasmid encoding a human EZH2 SET-domain-swap mutant containing the EZH1 SET domain

Depositor

Christopher Vakoc

Article

Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

Insert

KMT6 (EZH2 Human)

Use

Retroviral

Tags

None

Expression

Mutation

SET domain replaced with the SET domain of EZH1

Promoter

Available since

May 23, 2016

Availability

Academic Institutions and Nonprofits only
pBabe-TetCMV-puro-mVenus-PA-Rac1-T17N

Plasmid

#22018

Depositor

Klaus Hahn

Article

Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

Insert

mVenus-PA-Rac1-T17N (RAC1 Avena sativa (oat), Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Rac1 starts at I4 and contains mutation T17N.

Promoter

Available since

Sept. 29, 2009

Availability

Academic Institutions and Nonprofits only
pLV_mU6-sgNGN3_hU6-sgRNA_hUbC-PuroR-P2A-GFP

Plasmid

#162336

Purpose

Lentiviral expression of sgNGN3 paired with a second S. pyogenes sgRNA with a GFP-P2A-PuroR selection marker

Depositor

Charles Gersbach

Article

Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

Insert

S. pyogenes sgRNA

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6; mU6

Available since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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LentiU6-mHbb-bh1_ gRNA_1-MS2-Puro

Plasmid

#192681

Purpose

Lentiviral expression of sgRNA targeting mHbb-bh1promoter to activate mouse Hbb-bh1 transcription

Depositor

Jeffrey Miner

Article

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

Mouse Hbb-bh1 activating gRNA #1 (Hbb-bh1 Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pLe6Δ -hLP-mcs/(EGFP-IresPuro-hInt)

Plasmid

#64314

Purpose

chondrocyte-specific COL11A2 promoter/enhancer lentiviral reporter vector to select iChon cells

Depositor

Noriyuki Tsumaki

Article

Outani et al PLoS One. 2013 Oct 16;8(10):e77365. doi:

Inserts

COL11A2 promoter (COL11A2 Human)
COL11A2 enhancer (COL11A2 Human)

Use

Lentiviral

Tags

EGFP and IRES-PURO

Expression

Mammalian

Mutation

N/A

Promoter

Available since

April 29, 2015

Availability

Academic Institutions and Nonprofits only

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pBabe-TetCMV-puro-mPA-GFP-PA-Rac1

Plasmid

#22032

Depositor

Klaus Hahn

Article

Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

Insert

mPA-GFP-PA-Rac1 (RAC1 Avena sativa (oat), Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Rac1 starts at I4 and contains mutations Q61L, E9…

Promoter

Available since

Sept. 29, 2009

Availability

Academic Institutions and Nonprofits only
pLenti-3xFLAG-Puro PAK5 K478R K469R

Plasmid

#167180

Purpose

puromycin resistant lentiviral vector for expression of PAK5 K478R K469R

Depositor

Christin Burd

Article

LaPak et al Oncotarget. 2018 May 22;9(39):25386-2540

Insert

PAK5 K478R K469R

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

UBC

Available since

June 1, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti CMV Puro DEST (w118-1) RALB

Plasmid

#107506

Purpose

Expressing RALB, puromycin resistant

Depositor

Alejandro Gutierrez

Article

Aries et al J Exp Med. 2018 Nov 7. pii: jem.20180570

Insert

RALB (RALB Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti PGK Puro GFP-GABARAPL1 G116A

Plasmid

#123245

Purpose

Expression vector with PGK promoter for low expression of EGFP-GABARAPL1 G116A. For lentivirus production and stable transduction in mammalian cells.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Gamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Glycine 116 to Alanine

Promoter

PGK

Available since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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C-term Hec1-targeted Aurora B FRET sensor

Plasmid

#45222

Depositor

Michael Lampson

Article

Liu et al J Cell Biol. 2010 Mar 22;188(6):809-20.

Insert

C-term Hec1-targeted Aurora B FRET sensor

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 31, 2013

Availability

Academic Institutions and Nonprofits only

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BioID2-1x-ORF7b pBabe-puro (SARS-CoV-2)

Plasmid

#185992

Purpose

Expresses BioID2 fused to SARS-CoV-2 ORF7b and myc tag

Depositor

Kyle Roux

Article

May et al Viruses. 2022 Mar 15;14(3). pii: v140306

Insert

SARS-CoV-2 ORF7b (ORF7b SARS-CoV-2)

Use

Retroviral

Tags

BioID2 and myc

Expression

Mammalian

Mutation

Promoter

LTR

Available since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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ORF7a-1x-BioID2 pBabe-puro (SARS-CoV-2)

Plasmid

#185991

Purpose

Expresses SARS-CoV-2 ORF7a fused to BioID2 and HA tag

Depositor

Kyle Roux

Article

May et al Viruses. 2022 Mar 15;14(3). pii: v140306

Insert

SARS-CoV-2 ORF7a (ORF7a SARS-CoV-2)

Use

Retroviral

Tags

BioID2 and HA

Expression

Mammalian

Mutation

Promoter

LTR

Available since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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pMSCV-FLAG-2xSTREP-CCND2(T280A)-Puro

Plasmid

#172625

Purpose

Retroviral vector for the constitutive expression of FLAG-2xSTREP-tagged cyclin D2(T280A) and a puromycin resistance marker in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CCND2 (CCND2 Human)

Use

Retroviral

Tags

FLAG-2xSTREP

Expression

Mammalian

Mutation

Thr280Ala

Promoter

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pBabe-Tet-off-puro-mCherry-PA(wt)-Vav2

Plasmid

#91873

Purpose

Photo-activatable/light sensitive Vav2

Depositor

Klaus Hahn

Article

Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

Inserts

LOV2
VAV2 DH/PH/ZF (Vav2 Mouse)

Use

Tags

mCherry

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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1130 pBabe puroL HA FKHR H215R AAA

Plasmid

#9035

Depositor

William Sellers

Article

Ramaswamy et al Cancer Cell. 2002 Jul . 2(1):81-91.

Insert

FKHR H215R AAA (FOXO1 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

H215R T24A S256A S319A

Promoter

Available since

April 4, 2006

Availability

Academic Institutions and Nonprofits only
NSP2-1x-BioID2 pBabe-puro (SARS-CoV-2)

Plasmid

#185996

Purpose

Expresses SARS-CoV-2 NSP2 fused to BioID2 and HA tag

Depositor

Kyle Roux

Article

May et al Viruses. 2022 Mar 15;14(3). pii: v140306

Insert

SARS-CoV-2 NSP2 (ORF1ab SARS-CoV-2)

Use

Retroviral

Tags

BioID2 and HA

Expression

Mammalian

Mutation

Promoter

LTR

Available since

June 15, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti CMV Puro DEST (w118-1) CRIP2

Plasmid

#107509

Purpose

Expressing CRIP2, puromycin resistant

Depositor

Alejandro Gutierrez

Article

Aries et al J Exp Med. 2018 Nov 7. pii: jem.20180570

Insert

CRIP2 (codon optimized) (CRIP2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Codon optimized sequence

Promoter

CMV

Available since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-BE3-P2A-GFP-PGK-Puro

Plasmid

#110867

Purpose

Lentiviral vector for constitutive expression of BE3-P2A-GFP (not codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

BE3

Use

Lentiviral

Tags

Expression

Mutation

D10A

Promoter

EF1s

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pRP(Exp)-CMV> ORF_924bp (Azgp1):dTomato:IRES:Puro

Plasmid

#110831

Purpose

Expresses both zinc alpha-2 glycoprotein (ZAG) and dTomato (dT) reporter, where the fluorescent dT reporter is fused with the ZAG protein.

Depositor

Ande Satyanarayana

Article

Elattar et al FASEB J. 2018 Mar 23:fj201701465RR. doi:

Insert

Azgp1 (Azgp1 Mouse)

Use

Non-viral gene expression vector

Tags

dTomato

Expression

Mutation

Promoter

CMV

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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N-term Hec1-targeted Aurora B FRET sensor

Plasmid

#45239

Purpose

sensor targeted to N-terminus of Hec1

Depositor

Michael Lampson

Article

Welburn et al Mol Cell. 2010 May 14;38(3):383-92. doi:

Insert

Hec1, Aurora B FRET sensor

Use

Tags

Aurora B FRET sensor and Hec1

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 4, 2014

Availability

Academic Institutions and Nonprofits only

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