Addgene

We narrowed to 24,567 results for: sta

Showing: 2881 - 2900 of 24567 results

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pAG306-pTet07-synYFP[TTG/AGA]-6xArg[AGA]-nLuc

Plasmid

#199761

Purpose

Elongation reporter construct to quantify elongation duration of 6_AGA stalling

Depositor

Brian Zid

Article

Hou et al bioRxiv. 2023 Mar 20:2023.03.19.533377.

Insert

synYFP[TTG/AGA]-6xArg[AGA]-cLuc

Use

Tags

Expression

Yeast

Mutation

Promoter

TetO7

Available since

May 5, 2023

Availability

Academic Institutions and Nonprofits only

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pGL4.26_4xUAS_DSCP_GFP

Plasmid

#111931

Purpose

Vector to express GFP (under the control of the DSCP and an array of 4 UAS sites)

Depositor

Alexander Stark

Article

Arnold et al EMBO J. 2018 Aug 15;37(16). pii: embj.20

Insert

4xUAS GFP

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

April 19, 2023

Availability

Academic Institutions and Nonprofits only

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RPL13A_PQR_mScarlet_NOL_LoxP_PQR_zeo_PQR_MCS_RPL13A-3'UTR

Plasmid

#165043

Purpose

Repair template for CAS9 complex genome editing

Depositor

Brian Chen

Article

Lo et al Biotechniques. 2017 Apr 1;62(4):165-174.

Insert

RPL13A (RPL13A Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 29, 2021

Availability

Academic Institutions and Nonprofits only

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pJPS2951

Plasmid

#135585

Purpose

SNR6 has UU at position 105–106 deleted

Depositor

Jonathan Staley

Article

Toroney et al Genes Dev. 2019 Sep 26. pii: gad.328294.

Insert

snR6 (snR6 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

U6Δ2 has UU at position 105–106 deleted

Promoter

Native

Available since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pJPS2954

Plasmid

#135588

Purpose

SNR6 has UAUUUCat position 102–107 deleted

Depositor

Jonathan Staley

Article

Toroney et al Genes Dev. 2019 Sep 26. pii: gad.328294.

Insert

snR6 (snR6 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

U6Δ6 hasUAUUUCat position 102–107 deleted

Promoter

Native

Available since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pJPS2956

Plasmid

#135590

Purpose

SNR6 has GAUUUAUUUC at position 98–107 deleted

Depositor

Jonathan Staley

Article

Toroney et al Genes Dev. 2019 Sep 26. pii: gad.328294.

Insert

snR6 (snR6 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

U6Δ10 has GAUUUAUUUC at position 98–107 deleted

Promoter

Native

Available since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pJPS2957

Plasmid

#135591

Purpose

SNR6 has GAGAUUUAUUUC at position 96–107 deleted

Depositor

Jonathan Staley

Article

Toroney et al Genes Dev. 2019 Sep 26. pii: gad.328294.

Insert

snR6 (snR6 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

U6Δ12 has GAGAUUUAUUUC at position 96–107 deleted

Promoter

Native

Available since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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UL91-2xStrep pCDNA4

Plasmid

#120375

Purpose

Expresses HCMV UL91with Cterm 2xStrep tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

Insert

UL91 (UL91 Human Cytomegalovirus (HHV-5))

Use

Tags

2xStrep Tag II

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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Ppms028(JBEI-11601)

Plasmid

#87958

Purpose

jStack yeast assembly into plant vectors, Level 1 Intermediary Vector, p15A origin

Depositor

Dominique Loque

Article

Shih et al Nat Commun. 2016 Oct 26;7:13215. doi: 10

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 3, 2017

Availability

Academic Institutions and Nonprofits only

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pms5102(JBEI-10536)

Plasmid

#87969

Purpose

jStack yeast assembly into plant vectors

Depositor

Dominique Loque

Article

Shih et al Nat Commun. 2016 Oct 26;7:13215. doi: 10

Insert

{L_AtHsp18.2}

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 3, 2017

Availability

Academic Institutions and Nonprofits only

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pms5055(JBEI-10543)

Plasmid

#87962

Purpose

jStack yeast assembly into plant vectors

Depositor

Dominique Loque

Article

Shih et al Nat Commun. 2016 Oct 26;7:13215. doi: 10

Insert

{L_tOcs}

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 3, 2017

Availability

Academic Institutions and Nonprofits only

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pms5092(JBEI-14430)

Plasmid

#87968

Purpose

jStack yeast assembly into plant vectors

Depositor

Dominique Loque

Article

Shih et al Nat Commun. 2016 Oct 26;7:13215. doi: 10

Insert

{T_AtHsp18.2}

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 3, 2017

Availability

Academic Institutions and Nonprofits only

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pms5056(JBEI-10534)

Plasmid

#87963

Purpose

jStack yeast assembly into plant vectors

Depositor

Dominique Loque

Article

Shih et al Nat Commun. 2016 Oct 26;7:13215. doi: 10

Insert

{T_tNos}

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 3, 2017

Availability

Academic Institutions and Nonprofits only

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p3E-GFP-HA no-pA

Plasmid

#82589

Purpose

3' entry vector containing HA epitope fused to C-terminus of GFP without pA for imaging, epitope labeling/purification

Depositor

Kryn Stankunas

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

GFP-HA (no pA)

Use

Tags

Expression

Mutation

Promoter

None

Available since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pCfB2336

Plasmid

#67541

Purpose

EasyClone system-based yeast integrative vector carrying loxP-flanked SpHIS5 marker, integration into S. cerevisiae XII-5 chromosomal location, USER site for cloning, amp resistance

Depositor

Irina Borodina

Article

Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

Type

Empty backbone

Use

Cre/Lox; Integrative

Tags

Expression

Yeast

Mutation

Promoter

Available since

March 10, 2016

Availability

Academic Institutions and Nonprofits only

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pASKt15C+SrtA2woH

Plasmid

#65018

Purpose

Expresses the E105K/E108A mutant of Staphylococcus aureus sortase A in E. coli

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol J. 2015 Apr 10. doi: 10.1002/

Insert

sortase A

Use

Tags

S tag

Expression

Bacterial

Mutation

deleted amino acids 1-59, E105K, E108A

Promoter

tetracycline promoter/operator

Available since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-5'UTR-MAVS-ORF3,4(KaganE50)

Plasmid

#52007

Purpose

Expresses the human MAVS CDS and contains the 5'UTR sequence with a mutation in the start codons 61 and 67 nt downstream of the FL MAVS start codon

Depositor

Jonathan Kagan

Article

Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

Insert

5utr-MAVS-ORF2,3mut

Use

Tags

Expression

Mammalian

Mutation

mutated start codons for ORF3 and 4

Promoter

Available since

April 14, 2014

Availability

Academic Institutions and Nonprofits only
Oncorhynchus kisutch cryaa

Plasmid

#47667

Purpose

Recombinant protein production

Depositor

Mason Posner

Article

Posner et al PLoS One. 2012;7(3):e34438. doi: 10.1371

Insert

Alpha A-crystallin

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 21, 2013

Availability

Academic Institutions and Nonprofits only

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Pimephales notatus cryaa

Plasmid

#47621

Purpose

Recombinant protein production

Depositor

Mason Posner

Article

Posner et al PLoS One. 2012;7(3):e34438. doi: 10.1371

Insert

Alpha A-crystallin

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only

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Dissostichus mawsoni cryaa

Plasmid

#46167

Purpose

Recombinant protein production

Depositor

Mason Posner

Article

Posner et al PLoS One. 2012;7(3):e34438. doi: 10.1371

Insert

Alpha A-crystallin

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 27, 2013

Availability

Academic Institutions and Nonprofits only

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