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Showing: 2881 - 2900 of 13758 results

  1. Coexpressed Free ClLIS1 (M)

    Plasmid
    #182484
    Purpose
    Yeast integrative plasmid for expressing ERG20(F96W-N127W) (GAL10 promoter) and limonene synthase from Citrus limon (ClLIS1; GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3).
    Depositor
    Claudia Vickers
    Article
    Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.
    Inserts
    FPPS(M)
    LS
    Use
    Synthetic Biology; Metabolic engineering
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL10 and GAL7
    Available since
    Nov. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    182484-plasmid-map-sequence-id-360440

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  2. Lenti_EGFP-P2A-siRNAresHAUS6_IRES_Blast

    Plasmid
    #182887
    Purpose
    Transfer vector for production of lentivirus. Expresses EGFP-P2A-HAUS6 (siRNA resistant version)
    Depositor
    Daniel Gerlich
    Article
    David et al J Cell Biol. 2019 Jul 1;218(7):2150-2168
    Insert
    HAUS 6 (HAUS6 Human)
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Bacterial and Mammalian
    Mutation
    silet mutations in aa 197 - 203 to confer resista…
    Promoter
    EF1alpha core
    Available since
    July 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    182887-plasmid-map-sequence-id-367932

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  3. Lenti_EGFP-P2A_truncHaus6_IRES_Blast

    Plasmid
    #182885
    Purpose
    Transfer vector for production of lentivirus. Control plasmid for rescue experiment of HAUS6 depletion phenotype, containing a nonsense mutation and a truncation in HAUS6.
    Depositor
    Daniel Gerlich
    Article
    David et al J Cell Biol. 2019 Jul 1;218(7):2150-2168
    Insert
    HAUS 6 (HAUS6 Human)
    Use
    Lentiviral
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    stop codon introduced 2 amino acids downstream of…
    Promoter
    EF1alpha core
    Available since
    July 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    182885-plasmid-map-sequence-id-367930

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  5. pDestTol2-actinb-Hs.KCNJ13-IRES-EGFP

    Plasmid
    #164945
    Purpose
    Tol2 construct: actinb (actb2) promoter driven human KCNJ13 and EGFP”. This will reflect the linkage of KCNJ13 and EGFP
    Depositor
    GuangJun Zhang
    Article
    Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi
    Insert
    KCNJ13 (KCNJ13 Human)
    Use
    Tol2 transposon destination vector
    Tags
    IRES-EGFP
    Expression
    Mutation
    Stop codon deleted
    Promoter
    actinb (actb2) promoter
    Available since
    March 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    164945-plasmid-map-sequence-id-325850

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  6. pDestTol2-actinb-kcnj13-Q153H-EGFP

    Plasmid
    #164943
    Purpose
    Tol2 construct: actinb (actb2) promoter driven zebrafish loss-of-functionkcnj13 mutant, Q153H and M154I
    Depositor
    GuangJun Zhang
    Article
    Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi
    Insert
    kcnj13 (kcnj13 Zebrafish)
    Use
    Tol2 transposon destination vector
    Tags
    EGFP
    Expression
    Mutation
    Stop codon deleted; Q153H; M154I
    Promoter
    actinb (actb2) promoter
    Available since
    March 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    164943-plasmid-map-sequence-id-325844

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  7. pDestTol2-actinb-kcnj13-T131A-EGFP

    Plasmid
    #164942
    Purpose
    Tol2 construct: actinb (actb2) promoter driven zebrafish loss-of-function kcnj13 mutant, T131A and G133R
    Depositor
    GuangJun Zhang
    Article
    Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi
    Insert
    kcnj13 (kcnj13 Zebrafish)
    Use
    Tol2 transposon destination vector
    Tags
    EGFP
    Expression
    Mutation
    Stop codon deleted; T131A; G133R
    Promoter
    actinb (actb2) promoter
    Available since
    March 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    164942-plasmid-map-sequence-id-325785

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  8. GFP-Tac-STC(5A)

    Plasmid
    #162491
    Purpose
    Expresses non-O-glycosylated partial length Tac (stem region, TMD and cytosolic tail) with GFP tag in mammalian cells
    Depositor
    Lei Lu
    Article
    Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014
    Insert
    partial length Tac (stem region with mutations, TMD and cytosolic tail) (IL2RA Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Tac is in partial length with its stem region, TM…
    Promoter
    Available since
    Jan. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    162491-plasmid-map-sequence-id-323469

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  14. pHDR_G3BP1-V5-APEX2-mScarlet-I EF1a-Puro

    Plasmid
    #196196
    Purpose
    homologous recombination donor top insert V5-APEX2-mScarlet-I into the C-terminus of the human G3BP1 locus
    Depositor
    Jürgen Knoblich
    Article
    Sidhaye et al Elife. 2023 Mar 29;12:e85135. doi: 10.75
    Insert
    G3BP1 homology arms flanking V5-APEX2-mScarlet-I and EF1a-puro selection cassette (G3BP1 Human)
    Use
    Homology repair donor
    Tags
    V5-APEX2-mScarlet-I
    Expression
    Mutation
    Promoter
    Available since
    April 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    196196-plasmid-map-sequence-id-385065

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  16. pSH-Csy4-T2A-SpRFN

    Plasmid
    #85754
    Purpose
    Expresses S. pyogenes FokI-dCas9-NLS with a 25 amino acid linker (GGGGS)5 fusion. Also expresses Csy4 for cleavage of multiplexed gRNA transcripts. Derived from pSQT1601 (Addgene #53369).
    Depositor
    Lawrence Stanton
    Article
    Havlicek et al Mol Ther. 2017 Feb 1;25(2):342-355. doi:
    Insert
    Csy4-T2A-FokI-dCas9
    Use
    CRISPR
    Tags
    Csy4 and FokI fusion via 25 amino acid (GGGGS)5 l…
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Promoter
    CAG
    Available since
    Jan. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    85754-plasmid-map-sequence-id-170272

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  17. pSH-Csy4-T2A-SaRFN

    Plasmid
    #85755
    Purpose
    Expresses S.aureus FokI-dCas9-NLS with a 36 amino acid GSAT linker fusion. Also expresses Csy4 for cleavage of multiplexed gRNA transcripts. Backbone derived from pSQT1601 (Addgene #53369).
    Depositor
    Lawrence Stanton
    Article
    Havlicek et al Mol Ther. 2017 Feb 1;25(2):342-355. doi:
    Insert
    Csy4-T2A-FokI-SadCas9
    Use
    CRISPR
    Tags
    3xHA tag, Csy4, and FokI fusion via 36 amino acid…
    Expression
    Mammalian
    Mutation
    D10A, N580A
    Promoter
    CAG
    Available since
    Jan. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    85755-plasmid-map-sequence-id-170274

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  18. pTO_Snf7_LAP

    Plasmid
    #101851
    Purpose
    Tet-inducible expression of Snf7 from S. cerevisiae with a C-terminal GFP and long linker (LAP tag), compatible with Flp-In recombination for stable integration
    Depositor
    Daniel Gerlich
    Article
    Mierzwa et al Nat Cell Biol. 2017 Jul;19(7):787-798. d
    Insert
    SNF7 (SNF7 Budding Yeast)
    Use
    Gateway cloning, flp-frt recombination, tet-induc…
    Tags
    Localization and Affinity Purification (LAP) tag
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    101851-plasmid-map-sequence-id-200251

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Showing: 2881 - 2900 of 13758 results