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pGBK CUP1 Empty
Plasmid#169710PurposeYeast two-hybrid vector using a copper inducible CUP1 promoterDepositorArticleTypeEmpty backboneUseTagsGAL4 DNA Binding Domain and MycExpressionBacterial and YeastMutationPromoterCUP1Available sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-rUSP19ER-siRNAresistant
Plasmid#155251Purposefor mammalian overexpression of the siRNA resistant ER isoform of rat USP19DepositorInsertUSP19 (ER isoform) siRNA resistant (Usp19 Rat)
UseTags6XHis-3XFlagExpressionMammalianMutationLys(23)AAG to AAA, Lys(24)AAG to AAA, Gln(26)CAG …PromoterCMVAvailable sinceOct. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-rUSP19CYT-siRNAresistant
Plasmid#155252Purposefor mammalian overexpression of the siRNA resistant CYT isoform of rat USP19DepositorInsertUSP19 (CYT isoform) siRNA resistant (Usp19 Rat)
UseTags6XHis-3XFlagExpressionMammalianMutationLys(23)AAG to AAA, Lys(24)AAG to AAA, Gln(26)CAG …PromoterCMVAvailable sinceOct. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJK139
Plasmid#73315PurposePas2p peroxisomal membrane biogenesis proteinDepositorInsertPas2p peroxisomal membrane targeting protein
UseTagsHis6 and c-mycExpressionYeastMutationEncodes a glycine-204 to serine mutationPromoterAOX1Available sinceMarch 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
OMM-SPLICSs-nFAST
Plasmid#210430PurposeExpresses outer mitochondria-targeted split-NFAST for Short-range ER-Mito Contact sitesDepositorInsertOMM-SPLICSs-nFAST
UseTagsMycExpressionMammalianMutationPromoterAvailable sinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
221 pCS 3MT DEST
Plasmid#13070DepositorInsert6x Myc Tag
UseTagsExpressionMutationPromoterAvailable sinceJune 18, 2010AvailabilityAcademic Institutions and Nonprofits only -
ELL3 Streptag CtoA
Plasmid#127283PurposeExpresses mouse ELL3 with streptactin tag; To interrogate if the mutation decreases putative Myc activityDepositorInsertELL3 (NM_145973) (ELL3 Mouse)
UseTagsTwin strep-tagExpressionMammalianMutationCEYLHNK to AEYLHQKPromoterEF1aAvailable sinceAug. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pT2K-p53DN-T2A-copGFP
Plasmid#109229PurposeFor Tol2 transposon-mediated stable expression of dominant negative p53 and copGFPDepositorInsertdominant negative p53 (Trp53 Mouse)
UseTagscopGFPExpressionMammalianMutationdominant negative p53PromoterCAGGSAvailable sinceMay 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
LV-AR-KKKAAA
Plasmid#171219PurposeLenti-viral expression of human androgen receptor DNA-binding mutant: K630A-K632A-K633A, mutations at the boundary between AR DNA-binding domain and hinge domain.DepositorInsertAR-KKKAAA (AR Human)
UseLentiviralTagsFlagExpressionMammalianMutationAR-K630A-K632A-K633APromoterEF-1aAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
LV-AR-C563A-C596A
Plasmid#171220PurposeLenti-viral expression of human androgen receptor DNA-binding mutant: mutations of one essential cysteine on each of the two zinc fingers.DepositorInsertAR-C563A-C596A (AR Human)
UseLentiviralTagsFlagExpressionMammalianMutationAR-C563A-C596APromoterEF-1aAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
LV-AR-R599A-N600A
Plasmid#171221PurposeLenti-viral expression of human androgen receptor DNA-binding mutant: R599A-N600A, mutations in the D-box of the Zinc finger 2 that mediates AR dimerization.DepositorInsertAR-R599A-N600A (AR Human)
UseLentiviralTagsFlagExpressionMammalianMutationAR-R599A-N600APromoterEF-1aAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
LV-AR-AAFF
Plasmid#171218PurposeLenti-viral expression of human androgen receptor DNA-binding mutant: K580A-V581A, mutations in the P-box of the zinc finger 1 at the KVFF motif.DepositorInsertAR-AAFF (AR Human)
UseLentiviralTagsFlagExpressionMammalianMutationAR-K580A-V581APromoterEF-1aAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
SARS-CoV2 Nucleocapsid
Plasmid#153201PurposeExpresses myc-tagged nucleocapsid (N) from SARS-CoV2 in mammalian cellsDepositorInsertNucleocapsid from SARS-CoV-2 (N severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2))
UseTagsMyc tag (5X)ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 15, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
MSCV-human Erbb2-IRES-GFP
Plasmid#91888PurposeRetroviral expression of human Erbb2DepositorInsertErbb2 (ERBB2 Human)
UseRetroviralTagsIRES-EGFPExpressionMammalianMutationPromoterMMLV gagAvailable sinceJuly 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
Ath-HEN1::T2A::mCherry::TY1::H2B-pCFJ350-ttTi5605
Plasmid#108362PurposeExpression of 2xFlag MYC tagged A.thaliana-HEN1 (Ath-HEN1, codon optimised for C. elegans) and mCherry under the promoter of choice in C. elegansDepositorInsertsUseTags2xFlag, MYC, T2A peptide, and TY1 linkerExpressionWormMutationPromoterA rab-3:Ath-HEN1:unc-54 3' UTR was cloned as…Available sinceMay 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLXSN p110 CUX1
Plasmid#90471PurposeRetroviral vector expressing human p110 CUX1 (amino acids 747-1505) with a Myc and HA tag at the N- and C-terminue, respectivelyDepositorInsertCUX1 (amino acids 747-1505) (CUX1 Human)
UseRetroviralTagsHA and MycExpressionMammalianMutationPromoterMoloney murine leukemia virus long terminal repeatAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
AOI-WT-Cas9-sg-mouse Ezh2-E10-GFP
Plasmid#91879PurposeExpresses 3xFLAG-Cas9 and a gRNA targeting mouse Ezh2DepositorInsertgRNA targeting Ezh2 (Ezh2 Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJune 26, 2017AvailabilityAcademic Institutions and Nonprofits only
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