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pT2iApowriter2(#336)

Plasmid

#184104

Purpose

cyclofen-inducible apoptosis activation and imaging (tEosFP) in zebrafish transient transgenic

Depositor

Sophie Vriz

Article

Feng et al Rep Prog Phys. 2013 Jul;76(7):072601. do

Insert

myr-DIAP1-5myc-tEosFP-nls'-P2A-Casp9-ERT2

Use

Zebrafish transient transgenesis

Tags

internal tag: 5myc

Expression

Mutation

DIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …

Promoter

Zf-Ubi

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pT22Apowriter2(#340)

Plasmid

#184105

Purpose

cyclofen-inducible apoptosis activation and imaging (tEosFP) in zebrafish permanent transgenic

Depositor

Sophie Vriz

Article

Feng et al Rep Prog Phys. 2013 Jul;76(7):072601. do

Insert

myr-DIAP1-5myc-tEosFP-nls'-P2A-Casp9-ERT2

Use

Zebrafish transgenesis (blue eyes)

Tags

internal tag: 5myc

Expression

Mutation

DIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …

Promoter

Zf-Ubi

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pT22iuasvApoWtEos3N(#486)

Plasmid

#184107

Purpose

cyclofen-inducible apoptosis activation and imaging (tEosFP) in zebrafish permanent transgenic

Depositor

Sophie Vriz

Article

Feng et al Rep Prog Phys. 2013 Jul;76(7):072601. do

Insert

myr-DIAP1-2nls-tEosFP-nls'-P2A-Casp9-ERT2

Use

Zebrafish transgenesis (blue eyes)

Tags

Expression

Mutation

DIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …

Promoter

4xUAS

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pT22iuasvApoWChe(#488)

Plasmid

#184108

Purpose

cyclofen-inducible apoptosis activation and imaging (mCherry) in zebrafish permanent transgenic

Depositor

Sophie Vriz

Article

Feng et al Rep Prog Phys. 2013 Jul;76(7):072601. do

Insert

myr-DIAP1-nls'-mCherry-P2A-Casp9-ERT2

Use

Zebrafish transgenesis (blue eyes)

Tags

Expression

Mutation

DIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …

Promoter

4xUAS

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-BAF53b [N332B/15R]

Plasmid

#177533

Purpose

Mammalian Expression Plasmid of anti-BAF53b (Human). Derived from hybridoma N332B/15.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-BAF53b (Homo sapiens) recombinant mouse monoclonal antibody (Actl6b Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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Anti-Kir2.2 K+ channel [N124B/38R]

Plasmid

#140074

Purpose

Mammalian Expression Plasmid of anti-Kir2.2 K+ channel (Rat). Derived from hybridoma N124B/38.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Kir2.2 K+ channel (Rat) recombinant mouse monoclonal antibody (Kcnj12 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

dual CMV

Available since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223_IKZF5_WT

Plasmid

#81855

Purpose

Gateway Donor vector containing IKZF5 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

IKZF5 (IKZF5 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

M204V; p.415_418delQHNQ; p.419_420-insITNID

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_FAM116A_p.R427H

Plasmid

#81626

Purpose

Gateway Donor vector containing FAM116A , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

FAM116A (DENND6A Human)

Use

Gateway entry vector

Tags

Expression

Mutation

R427H

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_FBXO15_p.S366G

Plasmid

#81409

Purpose

Gateway Donor vector containing FBXO15 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

FBXO15 (FBXO15 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

S366G

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_IKZF5_p.E135K

Plasmid

#81384

Purpose

Gateway Donor vector containing IKZF5 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

IKZF5 (IKZF5 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

E135K; M204V; p.415_418delQHNQ; p.419_420-insITNID

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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HK3 gRNA (BRDN0001146645)

Plasmid

#75779

Purpose

3rd generation lentiviral gRNA plasmid targeting human HK3

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

HK3 (HK3, HOOK3 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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HK3 gRNA (BRDN0001148398)

Plasmid

#75780

Purpose

3rd generation lentiviral gRNA plasmid targeting human HK3

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

HK3 (HK3, HOOK3 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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HK3 gRNA (BRDN0001149028)

Plasmid

#75781

Purpose

3rd generation lentiviral gRNA plasmid targeting human HK3

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

HK3 (HK3, HOOK3 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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HK3 gRNA (BRDN0001149156)

Plasmid

#75782

Purpose

3rd generation lentiviral gRNA plasmid targeting human HK3

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

HK3 (HK3, HOOK3 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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CXCR4-SZ158a QTY

Plasmid

#162442

Purpose

Expression in E coli and perform ligand binding in solution

Depositor

Shuguang Zhang

Article

Qing et al iScience. 2020 Oct 28;23(12):101670. doi

Insert

C-X-C chemokine receptor type 4 (CXCR4 Human)

Use

Tags

his-tag, Rho-tag

Expression

Bacterial

Mutation

All L, I, V, F in the transmembrane region mutate…

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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CXCR4-SZ146a QTY

Plasmid

#162443

Purpose

Expression in E coli and perform ligand binding in solution

Depositor

Shuguang Zhang

Article

Qing et al iScience. 2020 Oct 28;23(12):101670. doi

Insert

C-X-C chemokine receptor type 4 (CXCR4 Human)

Use

Tags

his-tag, Rho-tag

Expression

Bacterial

Mutation

All L, I, V, F in the transmembrane region mutate…

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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CXCR4-SZ158a-sfGFP

Plasmid

#162446

Purpose

Expression in HEK293T cell and compete ligand singaling against full-length receptors

Depositor

Shuguang Zhang

Article

Qing et al iScience. 2020 Oct 28;23(12):101670. doi

Insert

C-X-C chemokine receptor type 4 (CXCR4 Human)

Use

Tags

sfGFP

Expression

Mammalian

Mutation

truncation from aa52 to aa257

Promoter

CMV

Available since

Availability

Academic Institutions and Nonprofits only

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PB-tetO-hNIL

Plasmid

#197089

Purpose

PiggyBac plasmid with tet-inducible expression of transcription factors for motor neuron differentiation

Depositor

Brian Wainger

Article

Held et al Cell Rep. 2023 Aug 30;42(9):113046. doi:

Insert

NGN2-P2A-ISL1-T2A-LHX3 (NEUROG2, ISL1, LHX3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

TRE3G

Available since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pLENTI_hACE2_HygR

Plasmid

#155296

Purpose

Lentiviral vector to generate hACE2 stable expressing cell line, Hygromycin resistance

Depositor

Raffaele De Francesco

Article

Notarbartolo et al Sci Immunol. 2021 Aug 10;6(62). pii: 6/6

Insert

hACE2 (ACE2 Human)

Use

Lentiviral

Tags

C9

Expression

Mutation

Promoter

CMV

Available since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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MSCV-N EBNA3A

Plasmid

#37956

Depositor

Karl Munger

Article

Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

Insert

EBNA3A (EBNA-3A H. Herpesvirus 4 (EBV))

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Mutation

Promoter

PKG

Available since

Aug. 2, 2012

Availability

Academic Institutions and Nonprofits only

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