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Plasmid#175064Purposeexpresses mutant of the huntingtin exon1 in mammalian cellsDepositorInsertpolyQ79 (HTT Human)
UseTagsExpressionMammalianMutationmVenus: C-Terminal deletion of 11 aa (GITLGMDELYK…PromoterCMV PromoterAvailable sinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
Sema3c(L)-AP-His
Plasmid#72015PurposeExpresses the Sema3C protein (truncated at cleavage site P3; ie, long), C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorInsertSema3c (Sema3c Mouse)
UseTagsAP-HisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-synp-F-H2B-jRGECO1a
Plasmid#102995PurposeExpresses histone fused jRGECO1a under the synapsin promoterDepositorInsertH2B-jRGECO1a
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-RIIα-Dronpa
Plasmid#182331PurposePKA RIIα subunit-targeted Dronpa. Fluctuating green-fluorescent label for live-cell super-resolution imaging.DepositorInsertRIIalpha-Dronpa (PRKAR2A Human)
UseTagsFull-length human PKA type 2alpha regulatory subu…ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-OTI-TCRB
Plasmid#131036PurposeExpresses OT-I TCRBDepositorArticleInsertTCRB (Tcrb Mouse)
UseTagsExpressionMammalianMutationPromoterCMV/T7Available sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMyosin-miRFP670nano3
Plasmid#184668PurposeLC-Myosin labelled with near-infrared fluorescent protein miRFP670nano3DepositorInsertmiRFP670nano3-LC-Myosin
UseTagsmiRFP670nano3ExpressionMammalianMutationPromoterCMVAvailable sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-MPP8-10xHis
Plasmid#194178PurposeExpresses MPP8-10xHis taggedDepositorInsertMPHOSPH8 (MPHOSPH8 Human)
UseTags10xHisExpressionMammalianMutationPromoterCMVAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET 15b-UbcH5a/2417
Plasmid#11472DepositorInsertUbcH5a (UBE2D1 Human)
UseTagsExpressionBacterialMutationn/aPromoterAvailable sinceApril 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
AAV-AIO-M11-gRNA-EFS-NMS-SadCas9
Plasmid#210710PurposeThis Plasmid express M11 promoter driven SadCas9 specific gRNA and EFS promoter driven NMS transactivation module fused to SadCas9DepositorInsertSadCas9 specific gRNA and NMS fused to N-terminus of SadCas9
UseAAV and CRISPRTagsFLAGExpressionMammalianMutationD10A/N580APromoterM11Available sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-mNeonGreen-LEHD-NanoLuc
Plasmid#98289Purposecaspase-9 BRET SensorDepositorInsertmNeonGreen-LEHD-NanoLuc
UseTagsHis-tagExpressionMammalianMutationPromoterCMVAvailable sinceApril 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYES2.1-pomMet8
Plasmid#107449PurposeOverexpression of MET fusion protein in S. cerevisiaeDepositorInsertMet8
UseTagsV5-HisExpressionYeastMutationPromoterGAL1Available sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-DAKAP-μDpTT
Plasmid#182324PurposeMitochondria-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.DepositorInsertDAKAP-μDronpa-TagRFP-T
UseTagsN-terminal 30 amino acids from DAKAP1ExpressionMammalianMutationDronpa contains H193T mutation.PromoterCMVAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pASR103
Plasmid#122637PurposeBglFusion compatible IPTG inducible integration vector for Streptococcus pneumoniae (pPEPZ Backbone)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceAug. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
RHPMP132 (mEOS3.1-beta Actin)
Plasmid#88836PurposeLocalization by Super-Resolution microscopy of beta-Actin fused to mEOS3.1DepositorInsertbeta-Actin (ACTB Human)
UseTagsmEOS3.1ExpressionMammalianMutationPromoterCMVAvailable sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
RHPMP138 (pLifeAct PA-mCH)
Plasmid#88838PurposeLocalization by Super-Resolution microscopy of lifeAct fused to PA-mCherryDepositorInsertLifeAct
UseTagsPA-mCHExpressionMammalianMutationPromoterCMVAvailable sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
RHPMP133 (mOrange2-beta Actin)
Plasmid#88837PurposeLocalization by Super-Resolution microscopy of beta-Actin fused to mOrange2DepositorInsertbeta-Actin (ACTB Human)
UseTagsmOrange2ExpressionMammalianMutationPromoterCMVAvailable sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
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TFORF3274
Plasmid#144750PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertHMGA1 (HMGA1 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
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mNeonGreen-IETD-NanoLuc
Plasmid#98288Purposecaspase-8 BRET SensorDepositorInsertmNeonGreen-LEHD-NanoLuc
UseTagsHis-tagExpressionMammalianMutationPromoterCMVAvailable sinceApril 11, 2019AvailabilityAcademic Institutions and Nonprofits only