Addgene

We narrowed to 3,641 results for: pEGFP

Showing: 2861 - 2880 of 3641 results

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pNESx2-EGFP-C1-PABDx2-Spo20

Plasmid

#220082

Purpose

PA biosensor with tandem Spo20-PABDs and two NES domains to aid in nuclear export

Depositor

Gerry Hammond

Article

Weckerly et al bioRxiv [Preprint]. 2024 Feb 28:2024.02.

Insert

Spo20 (SPO20 Budding Yeast)

Use

Tags

X. leavis map2k1.L(32-44)-GGSG-X. leavis map2k1.L…

Expression

Mammalian

Mutation

amino acids 51-91, tandem dimer

Promoter

CMV

Available since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-LAMP1-mCherry

Plasmid

#214274

Purpose

Mammalian expression of LAMP1-mCherry (lysosome marker)

Depositor

Shinya Tsukiji

Article

Ajiki et al RSC Chem Biol. 2024 Apr 24;5(6):544-555.

Insert

LAMP1-mCherry (Lamp1 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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Arf5-GFP

Plasmid

#39557

Depositor

Paul Melancon

Article

Chun et al Mol Biol Cell. 2008 Aug;19(8):3488-500.

Insert

ADP ribosylaion factor 5 (ARF5 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 10, 2012

Availability

Academic Institutions and Nonprofits only
aCat TL2 (1-697 mTFP1-TSmod-Venus STOP)

Plasmid

#101298

Purpose

FRET-based tension sensor (TS) technology to develop a probe for molecular-level tension across alphaE-catenin.

Depositor

Alpha Yap

Article

Acharya et al Cell Rep. 2017 Mar 21;18(12):2854-2867.

Insert

alpha-Catenin

Use

Tags

Expression

Mammalian

Mutation

Contains amino acids 1-698 of alpha-Catenin

Promoter

Available since

June 5, 2019

Availability

Academic Institutions and Nonprofits only

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yPet_cp229-Binder

Plasmid

#179137

Purpose

YPet circularly permuted at a.a. 229; Binder: SspB that carries two point mutations reducing homodimerization(Y11K/A15E, residue numbering from pdb: 1ou9).

Depositor

Klaus Hahn

Article

Liu et al Cell. 2021 Oct 28;184(22):5670-5685.e23.

Insert

SspB

Use

Tags

yPet_cp229

Expression

Mammalian

Mutation

Y11K/A15E, residue numbering from pdb: 1ou9

Promoter

Available since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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FKBP-beta2(616-951)-mRuby2

Plasmid

#100730

Purpose

To express a clathrin "hook" in mammalian cells to hot-wire endocytosis

Depositor

Stephen Royle

Article

Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

Insert

AP2B1 (AP2B1 Human)

Use

Tags

FKBP and mRuby2

Expression

Mammalian

Mutation

Long isoform

Promoter

CMV

Available since

Sept. 18, 2017

Availability

Academic Institutions and Nonprofits only

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dmBACCS2-IRES-dOrai-IRES-GFP

Plasmid

#72893

Purpose

Mammalian expression of dmBACCS2, a Drosophila melanogaster blue light-activated Ca2+ channel switch, along with dOrai and GFP

Depositor

Takao Nakata

Article

Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

Insert

dmBACCS2-IRES-dOrai-IRES-GFP

Use

Tags

IRES-GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 27, 2016

Availability

Academic Institutions and Nonprofits only

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anxA10-GFP

Plasmid

#107199

Purpose

Expresses human annexin A10-GFP fusion protein for live cell imaging

Depositor

Volker Gerke, Ursula Rescher

Article

Quiskamp et al Cell Mol Life Sci. 2014 Jan;71(2):311-29

Insert

annexin A10 (ANXA10 Human)

Use

Tags

Green Fluorescent Protein

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pH2R-P2A-mCherry-N1

Plasmid

#84331

Purpose

Co-expresses (untagged) Histamine-2 receptor and mCherry

Depositor

Dorus Gadella

Article

van Unen et al Mol Pharmacol. 2016 Sep;90(3):162-76. do

Insert

H2R-P2A-mCherry (HRH2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pCIBN-tagBFP-hPMLIII

Plasmid

#103807

Purpose

BLInCR 'Localizer' construct that marks the PML nuclear bodies and is targeted by a PHR-tagged effector upon illumination with blue light

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

CIBN-tagBFP-hPMLIII (CIB1, PML Human, Mustard Weed)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pmEos2-C1-centrin-1

Plasmid

#72588

Purpose

mEos2 photoconvertible fluorescent protein fused to the N-terminus of centrin-1

Depositor

Michel Bornens

Article

Sillibourne et al Cytoskeleton (Hoboken). 2011 Nov;68(11):

Insert

centrin 1 (CETN1 Human)

Use

Tags

mEos2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 4, 2016

Availability

Academic Institutions and Nonprofits only

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GFP-GLT1a-V5

Plasmid

#162517

Purpose

Rat GLT-1a cDNA N-terminally tagged with EGFP with a v5 tag in the extracellular loop

Depositor

Josef Kittler

Article

Al Awabdh et al Glia. 2016 Jul;64(7):1252-64. doi: 10.10

Insert

GLT1a (Slc1a2 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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HsB2AR-mCherry

Plasmid

#137785

Purpose

Visualization of the Beta-2-adrenergic receptor

Depositor

Dorus Gadella

Article

Pietraszewska-Bogiel et al ACS Omega. 2020 Feb 3;5(6):2648-2659. do

Insert

Human beta2-adrenergic receptor (ADRB2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pCIBN-tagBFP-TetR

Plasmid

#103797

Purpose

BLInCR 'Localizer' construct that marks tetO arrays and is targeted by a PHR-tagged effector upon illumination with blue light

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

CIBN-tagBFP-TetR (CIB1, tetR E. coli, Mustard Weed)

Use

Tags

Expression

Mammalian

Mutation

TetR: A4G (M2V)

Promoter

CMV

Available since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pH3R-P2A-mCherry-N1

Plasmid

#84332

Purpose

Co-expresses (untagged) Histamine-3 receptor and mCherry

Depositor

Dorus Gadella

Article

van Unen et al Mol Pharmacol. 2016 Sep;90(3):162-76. do

Insert

H3R-P2A-mCherry (HRH3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 9, 2016

Availability

Academic Institutions and Nonprofits only

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GFP-GLT1b-V5

Plasmid

#162518

Purpose

Rat GLT-1a cDNA N-terminally tagged with EGFP with a v5 tag in the extracellular loop

Depositor

Josef Kittler

Article

Al Awabdh et al Glia. 2016 Jul;64(7):1252-64. doi: 10.10

Insert

GLT1b (Slc1a2 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 16, 2021

Availability

Academic Institutions and Nonprofits only

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Dynactin-4/p62-3XHA

Plasmid

#31058

Depositor

Vann Bennett

Article

Ayalon et al J Biol Chem. 2011 Mar 4. 286(9):7370-8.

Insert

Dynactin-4 (DCTN4 Human)

Use

Tags

3XHA

Expression

Mammalian

Mutation

Promoter

Available since

March 5, 2012

Availability

Academic Institutions and Nonprofits only

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C1-F-tractin(delta1-6)-mCitrine

Plasmid

#155219

Purpose

F-actin bidning mutant of F-tractin

Depositor

Tobias Meyer

Article

Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

Insert

F-tractin (aa 9-52 of rat ITPKA) fused to mCitrine (Itpka Rat)

Use

Tags

mCitrine

Expression

Mammalian

Mutation

Deletion of first 6 AA from F-tractin (9-14 from …

Promoter

Available since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-Drosha1-390

Plasmid

#62521

Purpose

C-terminal aa391-1374 of Drosha is deleted

Depositor

Bharat Ramratnam

Article

Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

Insert

human Drosha with aa 391-1374 deleted (DROSHA Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

deleted amino acids 391-1374

Promoter

Available since

March 23, 2015

Availability

Academic Institutions and Nonprofits only
dmBACCS2VL-IRES-dOrai-IRES-mCherry

Plasmid

#72898

Purpose

Mammalian expression of dmBACCS2VL, a modified Drosophila melanogaster blue light-activated Ca2+ channel switch, along with dOrai and mCherry

Depositor

Takao Nakata

Article

Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

Insert

dmBACCS2VL-IRES-dOrai-IRES-mCherry

Use

Tags

IRES-mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 27, 2016

Availability

Academic Institutions and Nonprofits only

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