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pCfB2228

Plasmid

#67540

Purpose

EasyClone system-based yeast integrative vector carrying loxP-flanked SpHIS5 marker, integration into S. cerevisiae XII-4 chromosomal location, USER site for cloning, amp resistance

Depositor

Irina Borodina

Article

Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

Type

Empty backbone

Use

Cre/Lox; Integrative

Tags

Expression

Yeast

Mutation

Promoter

Available since

March 10, 2016

Availability

Academic Institutions and Nonprofits only

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p3E-YFP-HA no-pA

Plasmid

#82590

Purpose

3' entry vector containing HA epitope fused to C-terminus of YFP without pA for imaging, epitope labeling/purification

Depositor

Kryn Stankunas

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

YFP-HA (no pA)

Use

Tags

Expression

Mutation

Promoter

None

Available since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pMJ22

Plasmid

#63919

Purpose

Initial replicative vector

Depositor

Irene Castano Navarro

Article

Yanez-Carrillo et al Fungal Genet Biol. 2015 May 15. pii: S10

Type

Empty backbone

Use

Tags

NATMX

Expression

Yeast

Mutation

Promoter

Available since

July 6, 2015

Availability

Academic Institutions and Nonprofits only

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pSB1C3-hsg(M)

Plasmid

#138262

Purpose

Subcloning and expression of mCherry-targeting sgRNA M for use in Traffic Light reporter system

Depositor

Xiao Wang

Article

Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

Insert

sgRNA M

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

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AIRAP 4: AIRAP.GST.V3.pCDNA3

Plasmid

#21794

Depositor

David Ron

Article

Stanhill et al Mol Cell. 2006 Sep 15. 23(6):875-85.

Insert

AIRAP (Zfand2a Mouse)

Use

Tags

GST

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 10, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flag-Smad4 R100T

Plasmid

#80891

Purpose

mammalian expression of Smad4 R100T

Depositor

Aristidis Moustakas

Article

Moren et al Oncogene. 2000 Sep 7;19(38):4396-404.

Insert

Smad4 (SMAD4 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Pancreatic cancer mutation R100T

Promoter

CMV

Available since

Oct. 17, 2016

Availability

Academic Institutions and Nonprofits only
RSV-Pit1

Plasmid

#24059

Depositor

Frederick Stanley, Herbert Samuels

Article

Fox et al Mol Endocrinol. 1990 Jul . 4(7):1069-80.

Insert

Pit1 (SLC20A1 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2010

Availability

Academic Institutions and Nonprofits only
pBLIC-puro

Plasmid

#45197

Purpose

a ligation-independent cloning (LIC) retroviral vector for stable gene transduction in mammalian cells. Puro selection.

Depositor

Priyamvada Rai

Article

Patel et al BMC Biotechnol. 2012 Jan 16;12:3. doi: 1

Type

Empty backbone

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 19, 2015

Availability

Academic Institutions and Nonprofits only
pYC66

Plasmid

#63929

Purpose

Recipient replicative vector to construct MYC tagged fusions

Depositor

Irene Castano Navarro

Article

Yanez-Carrillo et al Fungal Genet Biol. 2015 May 15. pii: S10

Type

Empty backbone

Use

Tags

MYC

Expression

Yeast

Mutation

Promoter

Available since

May 4, 2015

Availability

Academic Institutions and Nonprofits only

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pCY 3050-07

Plasmid

#36223

Depositor

Anne Robinson

Article

Young et al Yeast. 2012 Mar;29(3-4):119-36. doi: 10.

Insert

Streptoalloteichus hindustanus bleomycin (Sh ble)

Use

Yeast cassette for pcr and homologous recombinati…

Tags

yEpolylinker-Venus-Zeocin

Expression

Mutation

Promoter

Available since

Aug. 9, 2012

Availability

Academic Institutions and Nonprofits only

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1xNLS-pMJ915v2-sfGFP

Plasmid

#88919

Purpose

For expression of modified SpyCas9 in e.coli.

Depositor

Jennifer Doudna

Article

Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Inserts

Cas9
T7

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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pGL3_ctrl_CP-candidate_luc+

Plasmid

#86392

Purpose

Luciferase validation vector, where candidates are inserted at the position of the core promoter. The candidates basal activity (no enhancer in vector) is reflected by luciferase activity

Depositor

Alexander Stark

Article

Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103

Type

Empty backbone

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

March 27, 2017

Availability

Academic Institutions and Nonprofits only

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pSUPERIOR.puro-shREV3-6

Plasmid

#38031

Depositor

Rolf Stahel, Emanuela Felley-Bosco

Article

Knobel et al Neoplasia. 2011 Oct;13(10):961-70.

Insert

shREV3-6 (REV3L Rat, Human, human)

Use

RNAi

Tags

Expression

Mutation

Promoter

Available since

Aug. 20, 2012

Availability

Academic Institutions and Nonprofits only
pSUPERIOR.puro-shREV3-5

Plasmid

#38030

Depositor

Rolf Stahel, Emanuela Felley-Bosco

Article

Knobel et al Neoplasia. 2011 Oct;13(10):961-70.

Insert

shREV3-5 (REV3L Rat, Human, human)

Use

RNAi

Tags

Expression

Mutation

Promoter

Available since

Aug. 20, 2012

Availability

Academic Institutions and Nonprofits only
2xNLS-pMJ915v2-sfGFP

Plasmid

#88920

Purpose

For expression of modified SpyCas9 in e.coli.

Depositor

Jennifer Doudna

Article

Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Inserts

Cas9
T7

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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pASKt15C+SrtA2

Plasmid

#64984

Purpose

Expresses the E105K/E108A mutant of Staphylococcus aureus sortase A in E. coli

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol J. 2015 Apr 10. doi: 10.1002/

Insert

sortase A

Use

Tags

His6 tag and S tag

Expression

Bacterial

Mutation

deleted amino acids 1-59, E105K, E108A

Promoter

tetracycline promoter/operator

Available since

June 9, 2015

Availability

Academic Institutions and Nonprofits only

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pASKt15C+SrtAwoH

Plasmid

#65017

Purpose

Expresses the wild type of Staphylococcus aureus sortase A in E. coli

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol J. 2015 Apr 10. doi: 10.1002/

Insert

sortase A

Use

Tags

S tag

Expression

Bacterial

Mutation

deleted amino acids 1-59

Promoter

tetracycline promoter/operator

Available since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flag-Smad4 P130S

Plasmid

#80892

Purpose

mammalian expression of Smad4 P130S

Depositor

Aristidis Moustakas

Article

Moren et al Oncogene. 2000 Sep 7;19(38):4396-404.

Insert

SMAD4 (SMAD4 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

colorectal cancer mutation P130S; additional D2E

Promoter

CMV

Available since

Dec. 2, 2016

Availability

Academic Institutions and Nonprofits only

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AIRAP 7: AIRAPmyc.pBABEpuro

Plasmid

#21797

Depositor

David Ron

Article

Stanhill et al Mol Cell. 2006 Sep 15. 23(6):875-85.

Insert

AIRAP (Zfand2a Mouse)

Use

Retroviral

Tags

myc

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 8, 2009

Availability

Academic Institutions and Nonprofits only

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pLP_mod_mNeonGreen11_P2A_puroR_CRE-GFP

Plasmid

#229227

Purpose

Modular BxB1 landing pad vector containing C-term mNeonGreen11 tag, puroR marker, and a cAMP GFP reporter.

Depositor

Willow Coyote-Maestas

Article

Howard et al bioRxiv [Preprint]. 2024 Apr 18:2024.04.

Type

Empty backbone

Use

Tags

mNeonGreen11

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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