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Plasmid#184149Purposeshort tracrRNA generator with WT anti-repeat regionDepositorInserts-tracrRNA-WT
UseSynthetic BiologyTagsExpressionBacterialMutationPromoteraraBAD promoterAvailable sinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLY283
Plasmid#184156PurposeReprogrammed short tracrRNA for hijacking mRNA of zntADepositorInserts-tracrRNA-zntA-1034
UseSynthetic BiologyTagsExpressionBacterialMutationPromoteraraBAD promoterAvailable sinceOct. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENTR-L3-Csy4-Cas9-EGFP-L2
Plasmid#141045PurposeMutisite Gateway mediated vector constructionDepositorInsertCsy4-2A-Cas9-2A-EGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR-L3-eSpCas9(1.1)-2A-EGFP-L2
Plasmid#141051PurposeMutisite Gateway mediated vector constructionDepositorInserteSpCas9(1.1)-2A-EGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR-L3-Csy4-FokI-dCas9-L2
Plasmid#141042PurposeMutisite Gateway mediated vector constructionDepositorInsertCsy4-2A-FokI-dCas9
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR-L3-Cas9n-EGFP-L2
Plasmid#141044PurposeMutisite Gateway mediated vector constructionDepositorInsertCas9n-2A-EGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR-L3-Csy4-Cas9n-L2
Plasmid#141041PurposeMutisite Gateway mediated vector constructionDepositorInsertCsy4-2A-Cas9n
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR-L3-Cas9-EGFP-L2
Plasmid#141043PurposeMutisite Gateway mediated vector constructionDepositorInsertCas9-2A-EGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR-L3-SpCas9-HF4-2A-EGFP-L2
Plasmid#141049PurposeMutisite Gateway mediated vector constructionDepositorInsertSpCas9-HF4-2A-EGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR-L3-SpCas9-HF2-2A-EGFP-L2
Plasmid#141050PurposeMutisite Gateway mediated vector constructionDepositorInsertSpCas9-HF2-2A-EGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR-L3-Csy4-FokI-dCas9-EGFP-L2
Plasmid#141047PurposeMutisite Gateway mediated vector constructionDepositorInsertCsy4-2A-FokI-dCas9-2A-EGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR-L3-Csy4-Cas9-L2
Plasmid#141040PurposeMutisite Gateway mediated vector constructionDepositorInsertCsy4-2A-Cas9
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
MS2-adRNA (5'GAC)
Plasmid#170127PurposeLentiviral vector carrying 2 copies of the MS2 adRNA targeting a GAC at the 5' end of the ADAR2 deaminase domain in plasmids #170124 and 170125DepositorInsertMS2-ADAR2(5'GAC)-MS2
UseLentiviralTagsExpressionMammalianMutationPromoterHuman U6 and mouse U6Available sinceAvailabilityAcademic Institutions and Nonprofits only -
MS2-adRNA (3'GAC)
Plasmid#170129PurposeLentiviral vector carrying 2 copies of the MS2 adRNA targeting a GAC at the 3' end of the ADAR2 deaminase domain in plasmids #170124 and 170125DepositorInsertMS2-ADAR2(3'GAC)-MS2
UseLentiviralTagsExpressionMammalianMutationPromoterHuman U6 and mouse U6Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pLenti_P2A_Blast
Plasmid#211365PurposeLentiviral expression vector for an insert of interest linked to a P2A-T2A blasticidin sequence. Produces virus very efficiently. Can also be used for regular transfectionsDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSFFV-dNme2Cas9
Plasmid#193190PurposeLentiviral vector expressing nuclease-dead Cas9 from Neisseria meningitis (dNme2)DepositorInsertdNme2Cas9
UseTagsNLS and NLS-3xHA-NLSExpressionMammalianMutationH588A, D16APromoterSFFVAvailable sinceJan. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pW267-lenti-spsgRNA-hsCDH1-sg1-pEF1s-NLS-mScarlet-I-P2A-BlastR
Plasmid#170816PurposeLentiviral vector to co-express a human CDH1 spsgRNA (sg1-Cdh1) with NLS-mScarlet-IDepositorInsertNLS-mScarlet-I-P2A-BlastR
UseLentiviralTagsExpressionMammalianMutationNAPromoterAvailable sinceJune 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pW301-lenti-spsgRNA-hsITGB1-pEF1s-NLS-mNeonGreen-P2A-BlastR
Plasmid#170817PurposeLentiviral vector to co-express a human ITGB1 spsgRNA with NLS-mScarlet-IDepositorInsertNLS-mNeonGreen-P2A-BlastR
UseLentiviralTagsExpressionMammalianMutationNAPromoterAvailable sinceJune 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
lenti-KRAS-G121C/G13A-nick sgRNA
Plasmid#214099PurposeLentiviral vector expressing nicking sgRNA to induce KRAS G12C or G13A mutation using PE3DepositorInsertKRAS G12C or G13A nicking sgRNA
UseLentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLentiGuide-PolB1-puro
Plasmid#177146PurposeLentiviral vector expressing PolB-gRNA-1 and a puromycin resistance cassetteDepositorInsertPolBgRNA1 (POLB Human)
UseLentiviralTagsExpressionMammalianMutationPromoterU6Available sinceDec. 16, 2021AvailabilityAcademic Institutions and Nonprofits only