Skip to main content
Addgene
Showing: 2841 - 2860 of 13337 results

  10. pCSDest2-2XNLS-SpCas9-WT-NLS-3XHA-NLS-TALentry

    Plasmid
    #69232
    Purpose
    Wild type SpCas9 expression plasmid to clone TALEs through BbsI site (generates compatible overhangs with Acc65I and BamHI sites)
    Depositor
    Scot Wolfe
    Article
    Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n
    Insert
    SpCas9
    Use
    CRISPR
    Tags
    2X NLS, BbsI TALE entry cassette, and NLS-3XHA-NL…
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    69232-plasmid-map-sequence-id-122311

    Enlarge

  11. 1203U-U6-gRNA-pCMV-wtCas9-NG-P2A-mcherry-3'dmDR

    Plasmid
    #221448
    Purpose
    Expresses wtCas9-NG with double processed direct repeats (15nt) and a non-target spacer in between at 3' end, mCherry tag, and the gRNA targeting HEK293T-site3
    Depositor
    Omar Akbari
    Inserts
    wtCas9-NG
    mCherry
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    221448-plasmid-map-sequence-id-437819

    Enlarge

  12. pFUGW hUbC-Sga-dCas9-KRAB-T2A-GFP

    Plasmid
    #227009
    Purpose
    Lentiviral Expression of dCas9-KRAB from S. gallolyticus fused to the repressive KRAB domain
    Depositor
    Charles Gersbach
    Insert
    dCas9
    Use
    CRISPR and Lentiviral
    Tags
    3xFLAG, SV40 NLS
    Expression
    Mutation
    Promoter
    UbC
    Available since
    Feb. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    227009-plasmid-map-sequence-id-449966

    Enlarge

  13. pFUGW hUbC-Sin-dCas9-KRAB-T2A-GFP

    Plasmid
    #227010
    Purpose
    Lentiviral Expression of dCas9-KRAB from S. iniae fused to the repressive KRAB domain
    Depositor
    Charles Gersbach
    Insert
    dCas9
    Use
    CRISPR and Lentiviral
    Tags
    3xFLAG, SV40 NLS
    Expression
    Mutation
    Promoter
    UbC
    Available since
    Feb. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    227010-plasmid-map-sequence-id-449964

    Enlarge

  14. pFUGW hUbC-Spa-dCas9-KRAB-T2A-GFP

    Plasmid
    #227011
    Purpose
    Lentiviral Expression of dCas9-KRAB from S. parasanguinis fused to the repressive KRAB domain
    Depositor
    Charles Gersbach
    Insert
    dCas9
    Use
    CRISPR and Lentiviral
    Tags
    3xFLAG, SV40 NLS
    Expression
    Mutation
    Promoter
    UbC
    Available since
    Feb. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    227011-plasmid-map-sequence-id-449967

    Enlarge

  15. pFUGW hUbC-Sub-dCas9-KRAB-T2A-GFP

    Plasmid
    #227012
    Purpose
    Lentiviral Expression of dCas9-KRAB from S. uberis fused to the repressive KRAB domain
    Depositor
    Charles Gersbach
    Insert
    dCas9
    Use
    CRISPR and Lentiviral
    Tags
    3xFLAG, SV40 NLS
    Expression
    Mutation
    Promoter
    UbC
    Available since
    Feb. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    227012-plasmid-map-sequence-id-449965

    Enlarge

  16. pX330-U6-(negative selection module)-Chimeric_BB-CBh-hSpCas9

    Plasmid
    #223321
    Purpose
    It contains a ccdB-CMr negative selection module flanked by BpiI (BbsI) sites that allows excluding clones without correct insertion of sgRNA. Human codon optimized Cas9 expressing plasmid.
    Depositor
    Jonathan Arias
    Article
    Petruskeviciute et al Stem Cell Res. 2024 Dec 12;82:103628. do
    Inserts
    SpCas9 codon optimized for human (cas9 Human, Synthetic)
    chloramphenicol resistance protein
    CcdB
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CBh
    Available since
    Dec. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    223321-plasmid-map-sequence-id-447743

    Enlarge

Showing: 2841 - 2860 of 13337 results