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Plasmid#121712PurposepMVP L5-L4 entry plasmid, contains eCFP-P2A for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term eCFP linked by P2A to gene of interest.DepositorInserteCFP-P2A (open)
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
KV001: pMVP (L5-L4) eYFP
Plasmid#121713PurposepMVP L5-L4 entry plasmid, contains eYFP for 4-component MultiSite Gateway Pro assembly. Allows N-term eYFP fusion to gene of interest.DepositorInserteYFP (open)
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
HB501: pMVP (L1-L4) RIP
Plasmid#121688PurposepMVP L1-L4 entry plasmid, contains rat Ins1 promoter (RIP) for 3-component MultiSite Gateway Pro assemblyDepositorInsertRat Ins1 promoter (RIP; β-cell specific)
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTBL251 GG sgRNA
Plasmid#126436PurposeTo target a protospacer with GG at the cut site.DepositorInsertspacer against human genome with GG at cut site
UseTagsExpressionMammalianMutationPromoterhuman U6Available sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTBL510 CA sgRNA
Plasmid#126430PurposeTo target a protospacer with CA at the cut site.DepositorInsertspacer against human genome with CA at cut site
UseTagsExpressionMammalianMutationPromoterhuman U6Available sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTBL401 GC sgRNA
Plasmid#126435PurposeTo target a protospacer with GC at the cut site.DepositorInsertspacer against human genome with GC at cut site
UseTagsExpressionMammalianMutationPromoterhuman U6Available sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTBL071 AG sgRNA
Plasmid#126428PurposeTo target a protospacer with AG at the cut site.DepositorInsertspacer against human genome with AG at cut site
UseTagsExpressionMammalianMutationPromoterhuman U6Available sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTBL402 AC sgRNA
Plasmid#126427PurposeTo target a protospacer with AC at the cut site.DepositorInsertspacer against human genome with AC at cut site
UseTagsExpressionMammalianMutationPromoterhuman U6Available sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTBL507 GA sgRNA
Plasmid#126434PurposeTo target a protospacer with GA at the cut site.DepositorInsertspacer against human genome with GA at cut site
UseTagsExpressionMammalianMutationPromoterhuman U6Available sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTBL512 CG sgRNA
Plasmid#126432PurposeTo target a protospacer with CG at the cut site.DepositorInsertspacer against human genome with CG at cut site
UseTagsExpressionMammalianMutationPromoterhuman U6Available sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTBL063 GT sgRNA
Plasmid#126437PurposeTo target a protospacer with GT at the cut site.DepositorInsertspacer against human genome with GT at cut site
UseTagsExpressionMammalianMutationPromoterhuman U6Available sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTBL516 CT sgRNA
Plasmid#126433PurposeTo target a protospacer with CT at the cut site.DepositorInsertspacer against human genome with CT at cut site
UseTagsExpressionMammalianMutationPromoterhuman U6Available sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTBL515 AA sgRNA
Plasmid#126426PurposeTo target a protospacer with AA at the cut site.DepositorInsertspacer against human genome with AA at cut site
UseTagsExpressionMammalianMutationPromoterhuman U6Available sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTBL509 AT sgRNA
Plasmid#126429PurposeTo target a protospacer with AT at the cut site.DepositorInsertspacer against human genome with AT at cut site
UseTagsExpressionMammalianMutationPromoterhuman U6Available sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTBL511 CC sgRNA
Plasmid#126431PurposeTo target a protospacer with CC at the cut site.DepositorInsertspacer against human genome with CC at cut site
UseTagsExpressionMammalianMutationPromoterhuman U6Available sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTBL508 TA sgRNA
Plasmid#126438PurposeTo target a protospacer with TA at the cut site.DepositorInsertspacer against human genome with TA at cut site
UseTagsExpressionMammalianMutationPromoterhuman U6Available sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTBL250 TC sgRNA
Plasmid#126439PurposeTo target a protospacer with TC at the cut site.DepositorInsertspacer against human genome with TC at cut site
UseTagsExpressionMammalianMutationPromoterhuman U6Available sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTBL514 TG sgRNA
Plasmid#126440PurposeTo target a protospacer with TG at the cut site.DepositorInsertspacer against human genome with TG at cut site
UseTagsExpressionMammalianMutationPromoterhuman U6Available sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTBL513 TT sgRNA
Plasmid#126441PurposeTo target a protospacer with TT at the cut site.DepositorInsertspacer against human genome with TT at cut site
UseTagsExpressionMammalianMutationPromoterhuman U6Available sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTBL322 GC
Plasmid#126950PurposeTo target a protospacer with GC at the cut site.DepositorInsertspacer against human genome with GC at cut site
UseTagsExpressionMammalianMutationPromoterhuman U6Available sinceMay 30, 2019AvailabilityAcademic Institutions and Nonprofits only