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Showing: 2841 - 2860 of 8613 results

  1. pmCellFree_KA1_SARS-COV-2_NSP13

    Plasmid
    #169386
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus NSP13 with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _NSP13
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Expression
    Mutation
    Promoter
    T7- CMV/CAG
    Available since
    July 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    169386-plasmid-map-sequence-id-336868

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  2. pmCellFree_KA1_SARS-COV-2_ORF9c

    Plasmid
    #169397
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus ORF9c with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _ORF9c
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Expression
    Mutation
    Promoter
    T7- CMV/CAG
    Available since
    July 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    169397-plasmid-map-sequence-id-336871

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  3. pmCellFree_KA1_SARS-COV-2_NSP14

    Plasmid
    #169387
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus NSP14 with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _NSP14
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Expression
    Mutation
    Promoter
    T7- CMV/CAG
    Available since
    June 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    169387-plasmid-map-sequence-id-336501

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  4. pmCellFree_KA1_SARS-COV-2_NSP7

    Plasmid
    #169381
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus NSP7 with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _NSP7
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Expression
    Mutation
    Promoter
    T7- CMV/CAG
    Available since
    June 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    169381-plasmid-map-sequence-id-336497

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  5. miABE

    Plasmid
    #205412
    Purpose
    Vector encoding human codon-optimized enhanced-activity nOgeuIscB fusion with TadA8e driven by CAG promoter, optimized _RNA (_RNA*) driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Yingsi Zhou
    Article
    Han et al Nat Methods. 2023 May 25. doi: 10.1038/s
    Insert
    pU6-_RNA*-pCAG-bpNLS-TadA8e(V106W)-32aa-OgeuIscB*(D61A)-GS-bpNLS-GS-TadA8e(V106W)-bpNLS-bGH polyA-pCMV-mCherry-AmpR
    Use
    Tags
    Expression
    Mammalian
    Mutation
    D61A+E85R+H369R+S387R+S457R
    Promoter
    hU6, CAG, CMV
    Available since
    Dec. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    205412-plasmid-map-sequence-id-416556

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  7. pX551-miniCMV-SpCas9D10A nickase

    Plasmid
    #216735
    Purpose
    Expresses SpCas9-D10A nickase from a miniCMV promoter. For AAV packaging. Derived from pX551-miniCMV-SpCas9, which was a gift from Alex Hewitt (Addgene #107031)
    Depositor
    Vincent Dion
    Article
    Alvaro et al bioRxiv
    Insert
    Cas9-D10A nickase
    Use
    AAV and CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    T7 and mini-CMV
    Available since
    April 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    216735-plasmid-map-sequence-id-430321

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  9. pAAV-nEF-SpCas9D10A nickase

    Plasmid
    #216736
    Purpose
    Expresses SpCas9-D10A nickase from a nEF promoter. For AAV packaging. Derived from pAAV-nEF-SpCas9 (Addgene #87115)
    Depositor
    Vincent Dion
    Article
    Alvaro et al bioRxiv
    Insert
    Cas9-D10A nickase
    Use
    AAV and CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    EF-1-alpha core
    Available since
    April 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    216736-plasmid-map-sequence-id-430323

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  11. pAAV-FLEX-tdTomato

    Plasmid
    #28306
    Depositor
    Edward Boyden
    Has Service
    AAV PHP.S, AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9
    Insert
    tdTomato
    Use
    AAV and Cre/Lox; Adeno-associated virus
    Tags
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    CAG
    Available since
    Sept. 7, 2012
    Availability
    Academic Institutions and Nonprofits only

  12. pSaCas9_GFP

    Plasmid
    #64709
    Purpose
    Co-expression of human codon-optimized Staphylococcus aureus Cas9 nuclease and GFP, plasmid optimized for expression in human pluripotent stem cells and other mammalian cells
    Depositor
    Kiran Musunuru
    Insert
    SaCas9-2A-GFP
    Use
    CRISPR
    Tags
    2A-GFP and 3xFLAG-SV40 NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    May 14, 2015
    Availability
    Academic Institutions and Nonprofits only
    64709-plasmid-map-sequence-id-322473

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  14. pmCellFree_KA1_SARS-COV-2_NSP16

    Plasmid
    #169389
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus NSP16 with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _NSP16
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Expression
    Mutation
    Codon optimized for Saccharomyces cerevisiae
    Promoter
    T7- CMV/CAG
    Available since
    July 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    169389-plasmid-map-sequence-id-336165

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  15. pmCellFree_KA1_SARS-COV-2_NSP10

    Plasmid
    #169384
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus NSP10 with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _NSP10
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Expression
    Mutation
    Codon optimized for Saccharomyces cerevisiae
    Promoter
    T7- CMV/CAG
    Available since
    July 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    169384-plasmid-map-sequence-id-336496

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  16. pmCellFree_KA1_SARS-COV-2_ORF3b

    Plasmid
    #169391
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus ORF3b with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _ORF3b
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Expression
    Mutation
    Codon optimized for Saccharomyces cerevisiae
    Promoter
    T7- CMV/CAG
    Available since
    July 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    169391-plasmid-map-sequence-id-336874

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  18. pcDNA-rUSP19ER-siRNAresistant

    Plasmid
    #155251
    Purpose
    for mammalian overexpression of the siRNA resistant ER isoform of rat USP19
    Depositor
    Simon Wing
    Insert
    USP19 (ER isoform) siRNA resistant (Usp19 Rat)
    Use
    Tags
    6XHis-3XFlag
    Expression
    Mammalian
    Mutation
    Lys(23)AAG to AAA, Lys(24)AAG to AAA, Gln(26)CAG …
    Promoter
    CMV
    Available since
    Oct. 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    155251-plasmid-map-sequence-id-313953

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  19. pcDNA-rUSP19CYT-siRNAresistant

    Plasmid
    #155252
    Purpose
    for mammalian overexpression of the siRNA resistant CYT isoform of rat USP19
    Depositor
    Simon Wing
    Insert
    USP19 (CYT isoform) siRNA resistant (Usp19 Rat)
    Use
    Tags
    6XHis-3XFlag
    Expression
    Mammalian
    Mutation
    Lys(23)AAG to AAA, Lys(24)AAG to AAA, Gln(26)CAG …
    Promoter
    CMV
    Available since
    Oct. 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    155252-plasmid-map-sequence-id-313955

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  20. pmCellFree_KA3_SARS-COV-2_RBD

    Plasmid
    #169405
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 RBD domain of S protein with C-term mCherry
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _RBD (S SARS-Cov2)
    Use
    Cell-free expression vector
    Tags
    mCherry
    Expression
    Mutation
    Promoter
    T7- CMV/CAG
    Available since
    July 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    169405-plasmid-map-sequence-id-336878

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Showing: 2841 - 2860 of 8613 results