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Plasmid#126748PurposeZebrafish slow myosin heavy chain promoter. Clone 667DepositorInsertsmyhc1 (smyhc1 Zebrafish)
UseMultisite gateway entry cloneTagsExpressionMutationPromoterAvailable sinceJune 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
4xnrUAS:MLS-KillerRed in pTol2pA2-acrys-EGFP-
Plasmid#115516PurposePlasmid driving expression of membrane bound KillerRed fluorophore under UAS response element, with a green lens reporterDepositorInsertMLS-KillerRed
UseGateway cloningTagsMLSExpressionMutationPromoterUASAvailable sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCS2-mCherry-Rab11a (Q70L)
Plasmid#184037PurposeExpresses constitutively active mCherry Rab11a in mammalian cells and zebrafish embryosDepositorInsertRab11
UseTagsmCherryExpressionMammalianMutationGlutamine 70 changed to LeucinePromoterCMV IE94Available sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
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p3E-rab5c-DN
Plasmid#109737PurposeMultisite gateway vector for 3' tagging with dominant negative rab5cDepositorInsertrab5c (rab5c Zebrafish)
UseZebrafish plasmidsTagsExpressionMutationdominant negativePromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
p3E-rab5c-CA
Plasmid#109738PurposeMultisite gateway vector for 3' tagging with constitutively active rab5cDepositorInsertrab5c (rab5c Zebrafish)
UseZebrafish plasmidsTagsExpressionMutationconstitutively activePromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCS2-mCherry-Rab11a (S25N)
Plasmid#184036PurposeExpresses dominate negative mCherry Rab11a in mammalian cells and zebrafish embryosDepositorInsertRab11
UseTagsmCherryExpressionMammalianMutationSerine 25 changed to AsparaginePromoterCMV IE94Available sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Rab8a in pCSDEST2
Plasmid#140880PurposeExpression of zebrafish GFP-tagged Rab8a in mammalian cells.DepositorInsertRab8a (rab8a Zebrafish)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pCLR2070
Plasmid#28063DepositorInsertFokI endonuclease
UseZebrafish expressionTagsFLAGExpressionMammalianMutationContains N496D point mutation in FokI domain, as …PromoterAvailable sinceJuly 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCLR2068
Plasmid#28062DepositorInsertFokI endonuclease
UseZebrafish expressionTagsFLAGExpressionMammalianMutationContains H537R point mutation in FokI domain, as …PromoterAvailable sinceJuly 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
p3E-rab32a
Plasmid#109689PurposeMultisite gateway vector for 3' tagging with rab32aDepositorInsertrab32a (rab32a Zebrafish)
UseZebrafish plasmidsTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pTol1:uas-myc-act-map2k2b-2a-tdTomato
Plasmid#104721PurposeUAS-driven expression of activated MEKDepositorInsertactivated map2k2b (map2k2b Zebrafish)
UseZebrafish transposonTags2A-tdTomato and myc tagExpressionMutationserines 221 and 225 to aspartic acidPromoter10xUASAvailable sinceJan. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pME-actn2
Plasmid#109550PurposeMultisite gateway vector for 5' tagging with alpha actinin 2DepositorInsertalpha actinin 2
UseZebrafish plasmidsTagsExpressionMutationPlease see Depositor CommentsPromoterAvailable sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -