Addgene

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pD649-HAsp-CLMP-Fc(DAPA)-AviTag-6xHis

Plasmid

#156645

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

CLMP (CLMP Human)

Use

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR223-FGFR1

Plasmid

#23922

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

FGFR1 (FGFR1 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

W211G and S351F compared to insert sequence provi…

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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PB513B-1/TRE-hHNF1β-hHNF4α-hHNF6-EF1α-Bla

Plasmid

#199551

Purpose

PiggyBac-based transposon vector plasmid which encodes the expression units of liver-enriched transcription factor genes (hHNF1β-hHNF4α-hHNF6) under control of the TRE/PCMVmin promoter

Depositor

Masamichi Kamihira

Article

Kitano et al Cells. 2022 Apr 1;11(7):1194. doi: 10.33

Insert

hHNF1β-hHNF4α-hHNF6 (ONECUT1, HNF4A, HNF1B Human)

Use

Piggybac transposon vector

Tags

Expression

Mammalian

Mutation

Promoter

TRE+CMVmin promoter

Available since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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FGFR2 3xFlag

Plasmid

#110063

Purpose

FGFR2 expression in mammalian cells (JCOPCO paper)

Depositor

Martin Fernandez-Zapico

Article

Egan et al JCO Precis Oncol. 2017;2017:PO.17.00018.

Insert

fibroblast growth factor receptor 2 (FGFR2 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

Dec. 13, 2018

Availability

Academic Institutions and Nonprofits only

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PB513B-1/TRE-hHNF1α-hHNF3β-hCEBPα-hCEBPβ-hCEBPγ-EF1α-Puro

Plasmid

#199550

Purpose

PiggyBac-based transposon vector plasmid which encodes the expression units of liver-enriched transcription factor genes (hHNF1α-hHNF3β-hCEBPα-hCEBPβ-hCEBP-γ) under control of the TRE/PCMVmin promoter

Depositor

Masamichi Kamihira

Article

Kitano et al Cells. 2022 Apr 1;11(7):1194. doi: 10.33

Insert

hHNF1α-hHNF3β-hCEBPα-hCEBPβ-hCEBPγ (HNF1A, FOXA2, CEBPB, CEBPA, CEBPG Human)

Use

Piggybac transposon vector

Tags

Expression

Mammalian

Mutation

Promoter

TRE+CMVmin promoter

Available since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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FGFR2 F276C 3xFlag

Plasmid

#110064

Purpose

FGFR2 F276C expression in mammalian cells (JCOPO paper)

Depositor

Martin Fernandez-Zapico

Article

Egan et al JCO Precis Oncol. 2017;2017:PO.17.00018.

Insert

fibroblast growth factor receptor 2 (FGFR2 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

mutated Phenylalanine 276 to Cysteine (F276C)

Promoter

CMV

Available since

May 3, 2018

Availability

Academic Institutions and Nonprofits only

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FGFR2 K41E 3xFlag

Plasmid

#110105

Purpose

expresses FGFR2 with a single amino acid change in mammalian cells

Depositor

Martin Fernandez-Zapico

Article

Egan et al JCO Precis Oncol. 2017;2017:PO.17.00018.

Insert

fibroblast growth factor receptor 2 (FGFR2 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

mutated Lysine 41 to Glutamic acid (K41E)

Promoter

CMV

Available since

May 3, 2018

Availability

Academic Institutions and Nonprofits only

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lentiCRISPRv2_ISG

Pooled Library

#125753

Purpose

Expresses gRNAs targeting human Interferon-Stimulated Genes

Depositor

Michael Emerman, Molly OhAinle

Article

Roesch et al Retrovirology. 2018 Mar 20;15(1):26. doi

Expression

Mammalian

Species

Homo sapiens

Use

CRISPR and Lentiviral

Available since

May 7, 2020

Availability

Academic Institutions and Nonprofits only
Mode #2 Library (14-3-3σ)

Pooled Library

#111706

Purpose

Encodes 14-3-3σ and human phosphopeptides fused to split mCherry for Hi-P

Depositor

Jesse Rinehart

Article

Barber et al Nat Biotechnol. 2018 Aug;36(7):638-644.

Expression

Bacterial

Species

Homo sapiens

Available since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only
Mode #2 Library (14-3-3β)

Pooled Library

#111705

Purpose

Encodes 14-3-3β and human phosphopeptides fused to split mCherry for Hi-P

Depositor

Jesse Rinehart

Article

Barber et al Nat Biotechnol. 2018 Aug;36(7):638-644.

Expression

Bacterial

Species

Homo sapiens

Available since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only
Mode #1 Library

Pooled Library

#111704

Purpose

Encodes GST-fused human phosphopeptides

Depositor

Jesse Rinehart

Article

Barber et al Nat Biotechnol. 2018 Aug;36(7):638-644.

Expression

Bacterial

Species

Homo sapiens

Available since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only
Mode #2 Library (NEDD4-2 WW2 domain)

Pooled Library

#111708

Purpose

Encodes NEDD4-2 WW2 domain and human phosphopeptides fused to split mCherry for Hi-P

Depositor

Jesse Rinehart

Article

Barber et al Nat Biotechnol. 2018 Aug;36(7):638-644.

Expression

Bacterial

Species

Homo sapiens

Available since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only
Mode #2 Library (NEDD4 WW2 domain)

Pooled Library

#111707

Purpose

Encodes NEDD4 WW2 domain and human phosphopeptides fused to split mCherry for Hi-P

Depositor

Jesse Rinehart

Article

Barber et al Nat Biotechnol. 2018 Aug;36(7):638-644.

Expression

Bacterial

Species

Homo sapiens

Available since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only
Lourido Toxoplasma CRISPR Library V1

Pooled Library

#80636

Purpose

Plasmid sgRNA library containing ten guides against each of the 8,158 predicted Toxoplasma gondii protein coding genes.

Depositor

Sebastian Lourido

Article

Sidik et al Cell. 2016 Sep 8;166(6):1423-1435.e12. d

Available since

Sept. 19, 2016

Availability

Academic Institutions and Nonprofits only
Human SLC KO Library

Pooled Library

#132552

Purpose

Expresses gRNAs for knockout of the human Solute Carrier family

Depositor

Giulio Superti-Furga

Article

Girardi et al Nat Chem Biol. 2020 Apr;16(4):469-478. d

Expression

Mammalian

Species

Homo sapiens

Use

CRISPR and Lentiviral

Available since

May 7, 2020

Availability

Academic Institutions and Nonprofits only
delta guaB

Bacterial Strain

#113653

Purpose

Base strain that the pDCAF plasmids (Addgene #113646-113652) must be in.

Depositor

Jeffrey Barrick

Article

Gutierrez et al Appl Environ Microbiol. 2019 Nov 14;85(2

Bacterial Resistance

Kanamycin

Available since

Nov. 18, 2019

Availability

Industry, Academic Institutions, and Nonprofits
ML14

Bacterial Strain

#61911

Purpose

C43(DE3)-based E. coli amino acid auxotrophic host strains used for selective isotope labeling. tyrA gene deleted.

Depositor

Toshio Iwasaki, Robert Gennis

Article

Lin et al Methods. 2011 Dec;55(4):370-8. doi: 10.1

Bacterial Resistance

None

Available since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
ML17

Bacterial Strain

#61912

Purpose

C43(DE3)-based E. coli amino acid auxotrophic host strains used for selective isotope labeling. glnA gene deleted.

Depositor

Toshio Iwasaki, Robert Gennis

Article

Lin et al Methods. 2011 Dec;55(4):370-8. doi: 10.1

Bacterial Resistance

None

Available since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
ML21

Bacterial Strain

#61914

Purpose

C43(DE3)-based E. coli amino acid auxotrophic host strains used for selective isotope labeling. hisG tyrA genes deleted.

Depositor

Toshio Iwasaki, Robert Gennis

Article

Lin et al Methods. 2011 Dec;55(4):370-8. doi: 10.1

Bacterial Resistance

None

Available since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
ML3

Bacterial Strain

#61907

Purpose

C43(DE3)-based E. coli amino acid auxotrophic host strains used for selective isotope labeling. hisG gene deleted.

Depositor

Toshio Iwasaki, Robert Gennis

Article

Lin et al Methods. 2011 Dec;55(4):370-8. doi: 10.1

Bacterial Resistance

Kanamycin

Available since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only