-
Plasmid#216322PurposeTransactivation of murine Myo7b gene via split dCas9-VPR (REVeRT system).DepositorInsertSplit Cas9-VPR + splice donor site + gRNAs targeting the murine Myo7b locus for transactivation
UseAAVTagsExpressionMutationPromoterCMVAvailable sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-5'Luciferase(split CAGGT)_BDlacZ-SV40polyA
Plasmid#216320PurposeSplit luciferase assay to test reconstitution via mRNA trans-splicing (REVeRT system).DepositorInsertSplit Luciferase + splice donor site
UseAAVTagsExpressionMutationPromoterCMVAvailable sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV2.1-CMV-5'Cer-BDlacZ-bGHpA
Plasmid#216318PurposeSplit fluorophore assay to test reconstitution via mRNA trans-splicing (REVeRT system).DepositorInsertsplit cerulean fluorescent protein + splice donor site
UseAAVTagsExpressionMutationPromoterCMVAvailable sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHES7-NLuc-2A-tdTomato
Plasmid#204348PurposeDonor vector to tag endogenous pig HES7 locusDepositorInsertNLuc-2A-tdTomato
UseTagsNanoLuc and tdTomatoExpressionMammalianMutationPromoterAvailable sinceJan. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pINSPECT:NEAT1-total_SigP:NLuc
Plasmid#197267PurposeDonor plasmid for CRISPR/Cas9-mediated insertion of INSPECT into human NEAT1 locus (both isoforms). Encodes for intronic IRES-driven SigP:NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette.DepositorInsertNEAT1-total homology arms
UseCRISPR, Luciferase, Synthetic Biology, and Unspec…TagsExpressionMammalianMutationPromoterAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUt-mNF-GFP
Plasmid#199220PurposeLPUtopia-7 matching RMCE donor plasmid with GFP Negative-Feedback circuit (mNF-GFP). Use BlastR for positive selection and HSV-TK for negative selection.DepositorInserthTetR::P2A::EGFP
UseTagsEGFPExpressionMammalianMutationPromoterCMV D2ir promoterAvailable sinceJune 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUt-mPF-GFP
Plasmid#199222PurposeLPUtopia-7 matching RMCE donor plasmid with GFP Positive-Feedback circuit (mPF-GFP). Use BlastR for positive selection and HSV-TK for negative selection.DepositorInsertrtTA-Advanced::P2A::EGFP
UseTagsEGFPExpressionMammalianMutationPromotertight TRE promoterAvailable sinceJune 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
eIF3a- pMK293 (mAID-mCherry2-Hygro) plasmid
Plasmid#192236Purposeknockin donor vector of mAID-mCherry2-Hygro to C-terminus of endogenous human eIF3aDepositorInserteIF3a-knockin-homology arm-mAID-mCherry2-Hygro (EIF3A Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
eIF3b- pMK289 (mAID-mClover-NeoR) plasmid
Plasmid#192237Purposeknockin donor vector of mAID-mClover-NeoR to C-terminus of endogenous human eIF3bDepositorInserteIF3b-knockin-homology arm-mAID-mClover-NeoR (EIF3B Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
eIF3b- pMK293 (mAID-mCherry2-Hygro) plasmid
Plasmid#192238Purposeknockin donor vector of mAID-mCherry2-Hygro to C-terminus of endogenous human eIF3bDepositorInserteIF3b-knockin-homology arm-mAID-mCherry2-Hygro (EIF3B Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-ZNF883
Plasmid#101608PurposeDonor Vector containing ZNF883 transcription factor, part of the Human TFome CollectionDepositorInsertZNF883 (ZNF883 Human)
UseGateway shuttling vectorTagsExpressionMutationLast nucleotide of stop codon removed to allow fo…PromoterAvailable sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-ZNF234
Plasmid#101611PurposeDonor Vector containing ZNF234 transcription factor, part of the Human TFome CollectionDepositorInsertZNF234 (ZNF234 Human)
UseGateway shuttling vectorTagsExpressionMutationLast nucleotide of stop codon removed to allow fo…PromoterAvailable sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-ZNF385A
Plasmid#101615PurposeDonor Vector containing ZNF385A transcription factor, part of the Human TFome CollectionDepositorInsertZNF385A (ZNF385A Human)
UseGateway shuttling vectorTagsExpressionMutationLast nucleotide of stop codon removed to allow fo…PromoterAvailable sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-ZNF233
Plasmid#101558PurposeDonor Vector containing ZNF233 transcription factor, part of the Human TFome CollectionDepositorInsertZNF233 (ZNF233 Human)
UseGateway shuttling vectorTagsExpressionMutationLast nucleotide of stop codon removed to allow fo…PromoterAvailable sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-ZNF516
Plasmid#101566PurposeDonor Vector containing ZNF516 transcription factor, part of the Human TFome CollectionDepositorInsertZNF516 (ZNF516 Human)
UseGateway shuttling vectorTagsExpressionMutationLast nucleotide of stop codon removed to allow fo…PromoterAvailable sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-ZNF579_isoform2
Plasmid#101569PurposeDonor Vector containing ZNF579 transcription factor, part of the Human TFome CollectionDepositorInsertZNF579 (ZNF579 Human)
UseGateway shuttling vectorTagsExpressionMutationLast nucleotide of stop codon removed to allow fo…PromoterAvailable sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-FOXD4L5
Plasmid#101492PurposeDonor Vector containing FOXD4L5 transcription factor, part of the Human TFome CollectionDepositorInsertFOXD4L5 (FOXD4L5 Human)
UseGateway shuttling vectorTagsExpressionMutationLast nucleotide of stop codon removed to allow fo…PromoterAvailable sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-FOXD4L1
Plasmid#101510PurposeDonor Vector containing FOXD4L1 transcription factor, part of the Human TFome CollectionDepositorInsertFOXD4L1 (FOXD4L1 Human)
UseGateway shuttling vectorTagsExpressionMutationLast nucleotide of stop codon removed to allow fo…PromoterAvailable sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-ZNF852_isoform1
Plasmid#101533PurposeDonor Vector containing ZNF852 transcription factor, part of the Human TFome CollectionDepositorInsertZNF852 (ZNF852 Human)
UseGateway shuttling vectorTagsExpressionMutationLast nucleotide of stop codon removed to allow fo…PromoterAvailable sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-ZNF208
Plasmid#101549PurposeDonor Vector containing ZNF208 transcription factor, part of the Human TFome CollectionDepositorInsertZNF208 (ZNF208 Human)
UseGateway shuttling vectorTagsExpressionMutationLast nucleotide of stop codon removed to allow fo…PromoterAvailable sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only