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Plasmid#215759PurposeHDR template to insert a truncated CD3-zeta-deficient CD19-CAR into CD247 exon 2 (enhanced expression by GSG-2A linker)DepositorUseTagsIgG1 CH3ExpressionBacterialMutationPromoterAvailable sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only
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pUC19-HDRT-CD3z-truncCAR(anti-CD19)
Plasmid#215758PurposeHDR template to insert a truncated CD3-zeta-deficient CD19-CAR into CD247 exon 2DepositorUseTagsIgG1 CH3ExpressionBacterialMutationPromoterAvailable sinceJune 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEX-EGFPL10a (AAV Retrograde)
Viral Prep#98747-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pAAV-FLEX-EGFPL10a (#98747). In addition to the viral particles, you will also receive purified pAAV-FLEX-EGFPL10a plasmid DNA. Cre-dependent EGFPL10a (EGFP-tagged ribosomal protein) expression for translating ribosome affinity purification (TRAP). These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsEGFP (Cre-dependent)Available sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-Archon1-KGC-GFP-ER2 (AAV8)
Viral Prep#115892-AAV8PurposeReady-to-use AAV8 particles produced from pAAV-Syn-Archon1-KGC-GFP-ER2 (#115892). In addition to the viral particles, you will also receive purified pAAV-Syn-Archon1-KGC-GFP-ER2 plasmid DNA. Syn-driven Archon1, an opsin-based fluorescent voltage indicator, fused to EGFP. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsGFPAvailable sinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-FLEX-rc [Archon1-KGC-GFP-ER2] (AAV8)
Viral Prep#115893-AAV8PurposeReady-to-use AAV8 particles produced from pAAV-Syn-FLEX-rc [Archon1-KGC-GFP-ER2] (#115893). In addition to the viral particles, you will also receive purified pAAV-Syn-FLEX-rc [Archon1-KGC-GFP-ER2] plasmid DNA. Syn-driven, Cre dependent Archon1, an opsin-based fluorescent voltage indicator, fused to EGFP. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsGFP (Cre-dependent)Available sinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
EcNGRfC
Bacterial Strain#48936PurposeBase line strain is heat shock induce-able lambda red recombination system for MAGE (Multiplex Recombination Genome Engineering)DepositorBacterial ResistanceSpectinomycinAvailable sinceDec. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-FLEX-Archon1-KGC-EGFP-ER2 (AAV5)
Viral Prep#108422-AAV5PurposeReady-to-use AAV5 particles produced from pAAV-CAG-FLEX-Archon1-KGC-EGFP-ER2 (#108422). In addition to the viral particles, you will also receive purified pAAV-CAG-FLEX-Archon1-KGC-EGFP-ER2 plasmid DNA. CAG-driven, Cre dependent Archon1, an opsin-based fluorescent voltage indicator, fused to EGFP. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsEGFP (Cre-dependent)Available sinceSept. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGT3
Plasmid#15825DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
EcNGRfEfC
Bacterial Strain#48937PurposeBase line strain is heat shock induce-able lambda red recombination system for MAGE (Multiplex Recombination Genome Engineering)DepositorBacterial ResistanceSpectinomycinAvailable sinceDec. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGLU_66
Plasmid#225769PurposeThis is the native promoter from the catP chloramphenicol resistance cassette.DepositorInsertPcatP_[abr]
UseSynthetic BiologyTagsExpressionMutationN/APromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRGH-12
Plasmid#12222DepositorInsertpRas189 Gly12 truncated
UseTagsExpressionBacterialMutationthe wild type was truncated with HindIII to 171aa…PromoterAvailable sinceSept. 18, 2006AvailabilityAcademic Institutions and Nonprofits only -
pRLH-12
Plasmid#12232DepositorInsertpRas189 Leu12 truncated
UseTagsExpressionBacterialMutationtruncated with HindIII to 171aa (from 189aa); Gly…PromoterAvailable sinceSept. 18, 2006AvailabilityAcademic Institutions and Nonprofits only -
pSico-shHDAC2
Plasmid#194983Purposeconditional knock down HDAC2DepositorInsertshHDAC2
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
PCDNA-Δ90AEGFP
Plasmid#26644DepositorInsertΔ90beta catenin
UseTagsEGFP-mut4ExpressionMammalianMutationDeletion of first 90 aa of beta cateninPromoterAvailable sinceNov. 8, 2010AvailabilityAcademic Institutions and Nonprofits only -
pRVH-12
Plasmid#12223DepositorInsertpRas171 Val12 truncated
UseTagsExpressionBacterialMutationGly12 to Val12; wild type truncated to 171 aa (fr…PromoterAvailable sinceSept. 18, 2006AvailabilityAcademic Institutions and Nonprofits only -
Clover-mRuby2-FRET-10
Plasmid#58169PurposeLocalization: Cytosolic FRET, Excitation: , Emission:DepositorTypeEmpty backboneUseTagsClover and mRuby2ExpressionMammalianMutationPromoterAvailable sinceApril 28, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
mTagBFP2-mEGFP-FRET
Plasmid#58173PurposeLocalization: Cytosolic FRET, Excitation: , Emission:DepositorTypeEmpty backboneUseTagsmEGFP and mTagBFP2ExpressionMammalianMutationPromoterAvailable sinceApril 28, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
mCerulean3-mVenus-FRET-10
Plasmid#58180PurposeLocalization: Cytosolic FRET, Excitation: , Emission:DepositorTypeEmpty backboneUseTagsmCerulean3 and mVenusExpressionMammalianMutationPromoterAvailable sinceApril 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
mTagBFP-GFP2-FRET
Plasmid#58172PurposeLocalization: Cytosolic FRET, Excitation: , Emission:DepositorTypeEmpty backboneUseTagsTagGFP2 and mTagBFPExpressionMammalianMutationPromoterAvailable sinceApril 28, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
mTagBFP-mEGFP-FRET
Plasmid#58181PurposeLocalization: Cytosolic FRET, Excitation: , Emission:DepositorTypeEmpty backboneUseTagsmEGFP and mTagBFPExpressionMammalianMutationPromoterAvailable sinceApril 28, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits