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Showing: 2821 - 2840 of 8613 results

  1. PB-GFP-Gal8

    Plasmid
    #127191
    Purpose
    Piggybac transposon plasmid with CAG promoter GFP-Galectin 8 (GFP-Gal8) fusion protein. Useful as genetically encoded endosomal escape sensor.
    Depositor
    Jordan Green
    Article
    Rui et al Sci Adv. 2019 Dec 6;5(12):eaay3255. doi:
    Insert
    GFP-Gal8 (LGALS8 Human)
    Use
    Tags
    Gal8 is fused to the c-terminus of GFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    127191-plasmid-map-sequence-id-248359

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  2. pAAV-CMV-SpCas9D10A nickase

    Plasmid
    #216737
    Purpose
    Expresses SpCas9-D10A nickase from a CMVd1 promoter. For AAV packaging. Derived from pAAV-CMV-SpCas9 (Addgene #113034)
    Depositor
    Vincent Dion
    Article
    Alvaro et al bioRxiv
    Insert
    Cas9-D10A nickase
    Use
    AAV and CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    CMVd1
    Available since
    April 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    216737-plasmid-map-sequence-id-430337

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  3. pSLQ10878

    Plasmid
    #183964
    Purpose
    CAG-hyperCas12a(nuclease active)-HA-P2A-mCherry-T2A-HygroR
    Depositor
    Stanley Qi
    Article
    Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d
    Insert
    HyperdCas12a
    Use
    CRISPR
    Tags
    HA, Hygromycin Resistance, and mCherry
    Expression
    Mammalian
    Mutation
    D156R, E292R, D235R, D350R
    Promoter
    CAG
    Available since
    June 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    183964-plasmid-map-sequence-id-363120

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  4. pSLQ10873

    Plasmid
    #183961
    Purpose
    U6-SKSKSO(crRNA array)-CAG-hyperdCas12a-HA-miniVPR
    Depositor
    Stanley Qi
    Article
    Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d
    Inserts
    HyperdCas12a
    poly-crRNA array targeting Sox2-Klf4-Oct4
    Use
    CRISPR
    Tags
    HA
    Expression
    Mammalian
    Mutation
    D832A, D156R, E292R, D235R, D350R
    Promoter
    CAG and U6
    Available since
    Aug. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    183961-plasmid-map-sequence-id-369669

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  6. pLV(gRNA)-CMV-eGFP-U6(sgCTG)

    Plasmid
    #216732
    Purpose
    Contains a eGFP gene along with a U6 promoter driving the sgCTG (target sequence: (CUG)6). Used with the HD iPSC-derived astrocytes
    Depositor
    Vincent Dion
    Article
    Alvaro et al bioRxiv
    Insert
    sgCTG lentiviral guide RNA with eGFP tag
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and U6
    Available since
    April 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    216732-plasmid-map-sequence-id-430316

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  7. pAAV-U6-sgCTG-CMV-GFP

    Plasmid
    #216734
    Purpose
    AAV plasmid containing sgCTG (target sequence: (CUG)6) driven by a U6 promoter and eGFP under the control of the CMV promoter. Used for in vivo experiments.
    Depositor
    Vincent Dion
    Article
    Alvaro et al bioRxiv
    Insert
    sgCTG
    Use
    AAV and CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 and CMV
    Available since
    April 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    216734-plasmid-map-sequence-id-430319

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  8. CaMKII-Flex-MCS-WPRE-SV40 - GCH20

    Plasmid
    #230824
    Purpose
    AAV cloning vector for CRE dependent expression under the CaMKII promoter.
    Depositor
    Kimberly Ritola
    Type
    Empty backbone
    Use
    AAV and Cre/Lox; Cloning vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    230824-plasmid-map-sequence-id-459118

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  9. EF1a-Flex-MCS-WPRE-SV40 - GCH14

    Plasmid
    #230821
    Purpose
    AAV cloning vector for CRE dependent expression under the EF1a promoter.
    Depositor
    Kimberly Ritola
    Type
    Empty backbone
    Use
    AAV and Cre/Lox; Cloning vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    230821-plasmid-map-sequence-id-457139

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  10. Syn-FLEX-MCS-WPRE-SV40 - GCH8

    Plasmid
    #230818
    Purpose
    AAV cloning vector for CRE dependent expression under the Syn promoter.
    Depositor
    Kimberly Ritola
    Type
    Empty backbone
    Use
    AAV and Cre/Lox; Cloning vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    230818-plasmid-map-sequence-id-457133

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  11. IGI-P0492 pHR-dCas9-NLS-VPR-mCherry

    Plasmid
    #102245
    Purpose
    Lentiviral expression of dCas9-VPR-mCherry fusion protein for CRISPRa.
    Depositor
    Jacob Corn
    Insert
    dCas9-VPR-mCherry
    Use
    CRISPR and Lentiviral
    Tags
    NLS-VPR-mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Oct. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    102245-plasmid-map-sequence-id-354473

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  12. pmCellFree_KA1_SARS-COV-2_NSP3

    Plasmid
    #169378
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus NSP3 with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _NSP3
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Expression
    Mutation
    Promoter
    T7- CMV/CAG
    Available since
    June 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    169378-plasmid-map-sequence-id-335064

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  13. pmCellFree_KA1_SARS-COV-2_ORF9b

    Plasmid
    #169396
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus ORF9bwith N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _ORF9b
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Expression
    Mutation
    Promoter
    T7- CMV/CAG
    Available since
    June 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    169396-plasmid-map-sequence-id-335426

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  14. pmCellFree_KA1_SARS-COV-2_NSP9

    Plasmid
    #169383
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus NSP9 with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _NSP9
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Expression
    Mutation
    Promoter
    T7- CMV/CAG
    Available since
    July 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    169383-plasmid-map-sequence-id-336869

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  15. pmCellFree_KA1_SARS-COV-2_NSP12

    Plasmid
    #169385
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus NSP12 with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _NSP12
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Expression
    Mutation
    Promoter
    T7- CMV/CAG
    Available since
    July 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    169385-plasmid-map-sequence-id-336500

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  16. pmCellFree_KA1_SARS-COV-2_NSP15

    Plasmid
    #169388
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus NSP15 with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _NSP15
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Expression
    Mutation
    Promoter
    T7- CMV/CAG
    Available since
    July 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    169388-plasmid-map-sequence-id-336873

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  17. pmCellFree_KA1_SARS-COV-2_NSP5

    Plasmid
    #169380
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus NSP5 with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _NSP5
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Expression
    Mutation
    Promoter
    T7- CMV/CAG
    Available since
    June 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    169380-plasmid-map-sequence-id-335068

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  18. pmCellFree_KA1_SARS-COV-2_NSP4

    Plasmid
    #169379
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus NSP4 with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _NSP4
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Expression
    Mutation
    Promoter
    T7- CMV/CAG
    Available since
    Aug. 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    169379-plasmid-map-sequence-id-339562

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  19. pmCellFree_KA1_SARS-COV-2_NSP8

    Plasmid
    #169382
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus NSP8 with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _NSP8
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Expression
    Mutation
    Promoter
    T7- CMV/CAG
    Available since
    July 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    169382-plasmid-map-sequence-id-336870

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  20. pmCellFree_KA1_SARS-COV-2_NSP2

    Plasmid
    #169377
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus NSP2 with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _NSP2
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Expression
    Mutation
    Promoter
    T7- CMV/CAG
    Available since
    June 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    169377-plasmid-map-sequence-id-335058

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Showing: 2821 - 2840 of 8613 results