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pJKR-H-mphR

Plasmid

#62559

Purpose

Macrolide biosensor. High copy plasmid that expresses GFP proportional to macrolide concentration.

Depositor

George Church

Article

Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

Insert

GFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pGW-Dronpa-Cav1.2

Plasmid

#182333

Purpose

L-type voltage-gated calcium channel α1C subunit tagged with Dronpa. Fluctuating green-fluorescent label for live-cell super-resolution imaging.

Depositor

Jin Zhang

Article

Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951

Insert

Cav1.2-Dronpa (CACNA1C Rabbit)

Use

Tags

Full-length rabbit Cav1.2.

Expression

Mammalian

Mutation

T1066Y, Q1070M

Promoter

CMV

Available since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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HH-gRNA-HDV-Shuttle-Vector

Plasmid

#169098

Purpose

Backbone plasmid to generate HH-gRNA-HDV segment

Depositor

Marianne Bronner

Article

Gandhi et al Development. 2021 Mar 9. pii: dev.193565

Insert

HH-gRNAbackbone-HDV

Use

Tags

Expression

Mammalian

Mutation

Promoter

NA

Available since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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Lenti-EF1alpha-dCas9-10xGCN4_Hygro

Plasmid

#192651

Purpose

3rd generation lenti vector encoding dCas9-10xGCN4 (Suntag) with 2A Hygro resistance marker

Depositor

Jeffrey Miner

Article

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

dCas9-10xGCN4

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

D10A and N863A in Cas9

Promoter

EF1a

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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P336-FBP_11

Plasmid

#139702

Purpose

T-DNA encoding Fungal Bioluminescent Pathway Components, control pathway, missing Luz

Depositor

Daniel Voytas

Article

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

pLHB1B1:NnCPH:tSlH4, p35S_Short_TMV 5':AsNPGA:tAtug7, pCmYLCV:NnH3H:AtuOcs, pCmYLCV:NnHisps:tHsp

Use

Luciferase

Tags

Expression

Plant

Mutation

Promoter

various

Available since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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pEMS1982

Plasmid

#49113

Purpose

AAV plasmid with CLDN5 (Ple261) promoter driving expression of iCre.

Depositor

Elizabeth Simpson

Article

Baumann et al Exp Eye Res. 2019 Jul 16:107728. doi: 10

Insert

ssAAV-Ple261-iCre

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CLDN5

Available since

May 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pFA6a-5FLAG-natMX6

Plasmid

#19343

Depositor

Eishi Noguchi

Article

Noguchi et al Biotechnol J. 2008 Aug 26. ():.

Insert

natMX6

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 25, 2008

Availability

Academic Institutions and Nonprofits only

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IDG_TMEM63A_OE_1

Plasmid

#161667

Purpose

Overexpresses TMEM63A

Depositor

Mike McManus

Insert

TMEM63A (TMEM63A Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 18, 2021

Availability

Academic Institutions and Nonprofits only

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Arf4-GFP

Plasmid

#39556

Depositor

Paul Melancon

Article

Chun et al Mol Biol Cell. 2008 Aug;19(8):3488-500.

Insert

ADP ribosylaion factor 4 (ARF4 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 10, 2012

Availability

Academic Institutions and Nonprofits only

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pRT802

Plasmid

#68113

Purpose

Integration vector derived from Streptomyces phage phiBT1. Kanamycin resistance gene, oriT, ColE1 origin of replication.

Depositor

Maggie Smith

Article

Gregory et al J Bacteriol. 2003 Sep;185(17):5320-3.

Inserts

aac3(IV)
aph11
oriT
attP
int

Use

Streptomyces integrating vector

Tags

Expression

Mutation

Promoter

Available since

July 9, 2019

Availability

Academic Institutions and Nonprofits only

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GFP-pcDNA3-PKCgamma-cys1Acys1B

Plasmid

#21190

Depositor

Tobias Meyer

Article

Oancea et al J Cell Biol. 1998 Feb 9. 140(3):485-98.

Insert

PKCgamma-cys1Acys1B (Prkcg Rat)

Use

Tags

GFP

Expression

Mammalian

Mutation

PKCgamma-cys1Acys1B (aa35 to aa152 of rat PKCgamm…

Promoter

Available since

July 15, 2009

Availability

Academic Institutions and Nonprofits only

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R620-M11-303: CMV51p> SARS-CoV-2 S(1-1213)-2P-T4f-His6

Plasmid

#170195

Purpose

Mammalian expression of SARS-CoV-2 soluble spike trimer protein

Depositor

Dominic Esposito

Article

Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

Insert

SARS-CoV-2 S (spike) (S SARS-CoV-2)

Use

Tags

His6

Expression

Mammalian

Mutation

Promoter

CMV51

Available since

Nov. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pMM553

Plasmid

#68543

Purpose

NanoLuc expressed constitutively from PBT1311, pNBU2 backbone, AmpR

Depositor

Timothy Lu, Christopher Voigt

Article

Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

Insert

NanoLuc

Use

Tags

Expression

Mutation

Promoter

Available since

Oct. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV4-HA

Plasmid

#27553

Depositor

Shao-Cong Sun

Article

Xiao et al J Biol Chem. 2000 Jul 14. 275(28):21081-

Type

Empty backbone

Use

Tags

3x HA

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only

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pBGPa-CMV-GFP-OSBP PH domain

Plasmid

#58724

Purpose

Expresses GFP-Tagged OSBP PH domain in mammalian cells

Depositor

Tim Levine

Article

Levine et al Curr Biol. 2002 Apr 30;12(9):695-704.

Insert

Oxysterol-binding Protein PH domain (OSBP Human)

Use

Tags

GFP and myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only
PH-Btk-mRuby2

Plasmid

#124657

Purpose

PI(3,4,5)P3 probe. PH domain of BTK fused with mRuby2

Depositor

Cheng-han Yu

Article

Zhang et al Mol Biol Cell. 2019 Mar 1;30(5):622-635.

Insert

BTK (BTK Human)

Use

Tags

mRuby2

Expression

Mammalian

Mutation

contains only the PH domain of Bruton tyrosine ki…

Promoter

Available since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pBITE-EGFRvIII-F265-OKT3

Plasmid

#190679

Purpose

Encodes an EGFRvIII-CD3 BITE protein (F265 clone)

Depositor

Scott McComb

Article

Shepherd et al PLoS One. 2023 Jun 22;18(6):e0273884. do

Insert

Anti-EGFRvIII-scFv-F265-modularized-OKT3-HisTag

Use

Lentiviral

Tags

mNeonGreen

Expression

Mammalian

Mutation

Promoter

EFS

Available since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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CAGGS-MCS-NLS-3XFLAG-V5

Plasmid

#196383

Purpose

Empty backbone vector to insert cDNA for strong expression in mammalian cells

Depositor

Mauro Calabrese

Article

Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10

Type

Empty backbone

Use

Tags

3xFLAG and V5

Expression

Bacterial and Mammalian

Mutation

Promoter

CAGGs

Available since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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NpY-mNeon

Plasmid

#186618

Purpose

Encodes Neuropeptide Y fused to mNeonGreen

Depositor

Gia Voeltz

Article

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Neuropeptide Y (NPY Human)

Use

Tags

mNeonGreen

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA3-Flag MKK6(K82A)

Plasmid

#13519

Depositor

Roger Davis

Article

Raingeaud et al Mol Cell Biol. 1996 Mar . 16(3):1247-55.

Insert

MKK6(K82A) (MAP2K6 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Lys-82Ala

Promoter

Available since

July 25, 2007

Availability

Academic Institutions and Nonprofits only

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