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Plasmid#87040Purposemammalian expression of SZT2 fragmentDepositorInsertSZT2 (SZT2 Human)
UseTagsHAExpressionMammalianMutationAA 2241-endPromoterCMVAvailable sinceMarch 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-SZT2 1-1021
Plasmid#87037Purposemammalian expression of SZT2 fragmentDepositorInsertSZT2 (SZT2 Human)
UseTagsHAExpressionMammalianMutationAA 1-1021PromoterCMVAvailable sinceMarch 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-FLAG-Kaptin
Plasmid#100511PurposeExpresses FLAG tagged Kaptin in mammalian cells to make lentivirusDepositorInsertKPTN (KPTN Human)
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterAvailable sinceNov. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
Ubiquitin (1-76; T66V, L67N)
Plasmid#110755PurposeBacterial expression of human Ubiquitin (1-76; T66V, L67N)DepositorInsertUBB (UBB Human)
UseTagsExpressionBacterialMutationT66V, L67NPromoterT7Available sinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCR3-vsv-CenpB 1-166-Sgo1 N61I
Plasmid#108498Purposeexpression of vsv-CenpB 1-166-Sgo1 N61I (no PP2A binding)DepositorInsertCenpB-Shugoshin 1 (SGO1 Human)
UseTagsVSVExpressionMammalianMutationCenpB 1-166 fusion before Sgo1, no PP2A binding N…PromoterCMVAvailable sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-22HT-MSI1(7-192)R53E/R59E/R61E
Plasmid#60355PurposeR53E R59E R61E triple mutant of the mouse MSI1 RBDDepositorInsertmouse MSI1 (7-192) (Msi1 Mouse)
UseTagsHis and TEV protease siteExpressionBacterialMutationR53E, R59E and R61E mutations; contains amino aci…PromoterT7Available sinceOct. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1A-Dm-miR-13b-1
Plasmid#22762DepositorInsertmicro RNA 13b-1 (mir-13b-1 Fly, primary transcript)
UseDrosophila expressionTagsExpressionMutationPromoterAvailable sinceJan. 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
myc-actin-NES-1
Plasmid#60618PurposeEncodes MYC tagged beta-actin with a mutation in the NESDepositorInsertBeta-actin (ACTB Human)
UseTagsMYCExpressionBacterial and MammalianMutationI178A, L180APromoterAvailable sinceNov. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
DCT.VV.Orf67
Plasmid#12755PurposeThis plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.DepositorInsertVV.Orf67
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 6, 2006AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9-2A-Puro-RAB7A-gRNA1 (PX459)
Plasmid#221551PurposeExpresses Cas9 and gRNA for disruption of Rab7A gene in human cellsDepositorInsertgRNA targeting human Rab7A (RAB7A Human)
UseTagsExpressionMammalianMutationPromoterU6Available sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPB-EF1A-Puro-mCherry-RAB7-S72A
Plasmid#221556PurposeExpresses Cherry-tagged human Rab7 with S72A mutation in mammalian cells. Compatible with piggybac transposase for genome integration.DepositorInsertRab7A (RAB7A Human)
UsePiggybacTagsmCherryExpressionMammalianMutationChange serine 72 to alaninePromoterEF1aAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9-2A-Puro-RAB7A-gRNA2 (PX459)
Plasmid#221552PurposeExpresses Cas9 and gRNA for disruption of Rab7A gene in human cellsDepositorInsertgRNA targeting human Rab7A (RAB7A Human)
UseTagsExpressionMammalianMutationPromoterU6Available sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-2A-Puro-C9orf72
Plasmid#83439PurposeExpresses Cas9 and a gRNA targeting C9orf72DepositorInsertgRNA targeting human C9orf72 (C9orf72 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV_PGK_LTB4R(T308A)
Plasmid#208088PurposeMammalian expression of the human LTB4 receptor LTB4R with the T308A mutation.DepositorInsertLTB4R(T308A) (LTB4R Human)
UseLentiviralTagsExpressionMammalianMutationT308APromoterPGKAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV_PGK_LTB4R(S310A)
Plasmid#208089PurposeMammalian expression of the human LTB4 receptor LTB4R with the S310A mutation.DepositorInsertLTB4R(S310A) (LTB4R Human)
UseLentiviralTagsExpressionMammalianMutationS310APromoterPGKAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV_PGK_LTB4R(T308A,S310A)
Plasmid#208090PurposeMammalian expression of the human LTB4 receptor LTB4R with the T308A and S310A mutations.DepositorInsertLTB4R(T308A,S310A) (LTB4R Human)
UseLentiviralTagsExpressionMammalianMutationT308A, S310APromoterPGKAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pROF018
Plasmid#155325PurposeConstitutive expression of KRAB-EL222 in plantsDepositorInsertPCaMV35S-NLS-KRAB-EL222-Tnos
UseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pROF050
Plasmid#155327PurposeConstitutive expression of BRD-EL222 in plantsDepositorInsertPCaMV35S-BRD-NLS-EL222-Tnos
UseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-IRES-Puro GFP-3xFLAG
Plasmid#201636PurposeOver-expression of 3xFLAG-tagged GFPDepositorInsertGFP
UseLentiviralTags3xFLAGExpressionMutationPromoterAvailable sinceJuly 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJL1-MBP-DQNAT
Plasmid#198215PurposepJL1 plasmid encoding E. coli maltose binding protein (MBP) modified at the C-terminus with 30 amino acids containing one optimal DQNAT sequon followed by a 6x-His tagDepositorInsertMBP_DQNAT
UseTags6x His Tag and DQNAT glycosylation tagExpressionBacterialMutationPromoterT7Available sinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only