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Plasmid#127619PurposeN-terminal myc-tagged Gamma2 subunit of GABAARDepositorInsertGABA-A receptor subunit gamma-2
UseTagsmycExpressionMammalianMutationPromoterAvailable sinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pluc2-iRFP720
Plasmid#98887PurposeMammalian expressionDepositorInsertFirefly Luciferase
UseTagsiRFP720ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
hPDI1-18-508_C400S_C56S-pFLAG-CMV1
Plasmid#31383DepositorInsertPDI1A (P4HB Human)
UseTagsFLAG M2ExpressionMammalianMutationC-terminal cysteines of both active sites mutated…PromoterAvailable sinceMay 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
mCherry-FKBP-SAC1C389S
Plasmid#108128PurposeRecruitable inactive SAC1DepositorInsertmCherry:FKBP1A(3-108):[GGSA]4GG:SACM1LC389S (SACM1L Human)
UseTagsExpressionMammalianMutationSACM1L(C389S)PromoterCMVAvailable sinceApril 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pWXLd-RIEP-GpNLuc
Plasmid#135935PurposeExpresses the GpNLuc LumiFluor in mammalian cells.DepositorInsertGpNLuc LumiFluor
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pICH47732:PtFCP:ShBle
Plasmid#104893PurposeExpresses ShBle with FCP promoter/terminator, Golden Gate Assembly, for Phaeodactylum transformation.DepositorInsertShBle
UsePhaeodactylum; golden gate assemblyTagsPtFCP promoter and terminatorExpressionMutationPromoterAvailable sinceDec. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLG1-puro Non-targeting sgRNA 3
Plasmid#109003PurposeCRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)DepositorInsertNon-targeting sgRNA #3
UseLentiviralTagsExpressionMammalianMutationPromotermU6 promoterAvailable sinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
BiP 2: C. elegans BiP (hsp-4)-GFP
Plasmid#21896DepositorInsertBiP (hsp-4 Nematode)
UseTagsGFPExpressionWormMutationFusion between the GFP and hsp-4 takes place at A…PromoterAvailable sinceSept. 17, 2009AvailabilityAcademic Institutions and Nonprofits only -
pET28a-2xMreBN-mCherry-His
Plasmid#133626PurposeExpression vector for mCherry fused to a tandem repeat of the E. coli MreB membrane targeting sequence (MTS) (N-terminal fusion) and a C-terminal His-tag for purification.DepositorInsert2xMreB-mCherry-His
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
FL-mCherry
Plasmid#78862PurposeDULIP negative control (prey) to exclude unspecific background bindingDepositorInsertmCherry
UseExpression vectorTagsFirefly luciferase, V5-tagExpressionMammalianMutationPromoterCMVAvailable sinceJuly 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSB700 lenti gRNA EBFP2
Plasmid#167905PurposeLentiviral vector for expressing U6 sgRNA cloning plasmidDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGL3-p27Luc
Plasmid#23047DepositorInsertp27 (CDKN1B Human)
UseTagsLuciferaseExpressionMammalianMutationwt cDNAPromoterAvailable sinceJan. 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
pLG1-puro Non-targeting sgRNA 1
Plasmid#109002PurposeCRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)DepositorInsertNon-targeting sgRNA #1
UseLentiviralTagsExpressionMammalianMutationPromotermU6 promoterAvailable sinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV_HaloK73T_cyto
Plasmid#183687PurposeMammalian expression of cytosolic metastable Halotag/folding sensor (Cyto HaloK73T)DepositorInsertCyto Halo(K73T)
UseTagsExpressionMammalianMutationK73T in HalotagPromoterAvailable sinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
SP_H6_Halo_K73T_KDEL_pBABEpu
Plasmid#183689PurposeRetroviral expression of ER-targeted metastable Halotag/folding sensor (HaloK73T)DepositorInsertER Halo(K73T)
UseRetroviralTagsExpressionMutationK73T in HalotagPromoterAvailable sinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pWXLd-RIEP-OgNLuc
Plasmid#135936PurposeExpresses the OgNLuc LumiFluor in mammalian cells.DepositorInsertOgNLuc LumiFluor
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
mNeonGreen-IETD-NanoLuc
Plasmid#98288Purposecaspase-8 BRET SensorDepositorInsertmNeonGreen-LEHD-NanoLuc
UseTagsHis-tagExpressionMammalianMutationPromoterCMVAvailable sinceApril 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ybbR-HIS-sfGFP-DocI
Plasmid#58708PurposeCharacteristic fingerprint molecule to perform single molecule force spectroscopy experiments; covalently immobilized via ybbr tagDepositorInsertsfGFP-DocI
UseTagsHIS, HRV 3C, and ybbRExpressionBacterialMutationPromoterT7Available sinceAug. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCEP4 Smad2
Plasmid#16484DepositorInsertSmad2 (SMAD2 Human)
UseTagsDouble HAExpressionMammalianMutationPromoterAvailable sinceMarch 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
pTH
Plasmid#56659PurposeDual-expression vector for P. tricornutumDepositorInsertKanR
UseSynthetic Biology; Diatom expressionTagsExpressionMutationPromoterAvailable sinceOct. 14, 2014AvailabilityAcademic Institutions and Nonprofits only