-
Plasmid#204514PurposeBacterial expression of SYP-1 N-terminal fragment with 6xHis and maltose binding protein tags for use as an antigenDepositorInsertN-terminal fragment of syp-1 (C. elegans)
UseTags6xHis, MBPExpressionBacterialMutationPromoterT7lacAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28_redesigned_Caspase-4_ANR261ENP_D315H_M318K_T352K_enzymatic domain
Plasmid#183387PurposeOverexpression of catalytic domain of inflammatory caspase in bacteriaDepositorInsertredesigned Caspase-4 (AA94-377) (CASP4 Human)
UseTagsHis-TEVExpressionBacterialMutationANR261ENP, D315H, M318K, T352KPromoterT7Available sinceJuly 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-GFP-mouse-Caspase-1-H340D
Plasmid#183362PurposeStable expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transductionDepositorInsertCaspase-1 H340D (Casp1 Mouse)
UseRetroviralTagsMycExpressionMutationH340DPromoterMESVAvailable sinceJuly 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-GFP-dog-Caspase-1/4a-triple mutant-H342D_K345M_K379T
Plasmid#183364PurposeStable expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transductionDepositorInsertCaspase-1/4a (CASP4 Canis lupus familiaris)
UseRetroviralTagsMycExpressionMutationH342D_K345M_K379TPromoterMESVAvailable sinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-GFP-dog-Caspase-1/4b
Plasmid#183365PurposeStable expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transductionDepositorInsertCaspase-1/4b (CASP4 Canis lupus familiaris)
UseRetroviralTagsMycExpressionMutationPromoterMESVAvailable sinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-GFP-dog-Caspase-1/4a
Plasmid#183363PurposeStable expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transductionDepositorInsertCaspase-1/4a (CASP4 Canis lupus familiaris)
UseRetroviralTagsMycExpressionMutationPromoterMESVAvailable sinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
-
pJH2198
Plasmid#179773PurposePrgef-1 NLF-1::GFP unc-54 3' UTR C.elegans pan-neural expression of NLF-1::GFPDepositorInsertNLF-1::GFP (nlf-1 Nematode)
UseTagsGFPExpressionWormMutationPromoterPrgef-1Available sinceMarch 14, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
-
pRS426-CeLACE-1
Plasmid#174523PurposeExpress C.elegans LACE-1 in yeast cellsDepositorInsertlace-1 (C30F12.2 Nematode)
UseTags6xHisExpressionYeastMutationPromoterMET25Available sinceSept. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJJF152_Phsp_Cas9
Plasmid#163862Purposeheat shock-induced expression of Cas9 in C. elegansDepositorInsertCas9, C. elegans codon optimized with intron and SV40_NLS
UseCRISPRTagsExpressionWormMutationPromoterPhsp-16.48Available sinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
RCP83
Plasmid#163466PurposeBackbone plasmid for insertion of the Saccharomyces cerevisiae promoter library and yeGFPDepositorInsertsPromoter fragment (Sharon2012_1922)
His3 minimal promoter
yeGFP
UseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2113 - [4-1] - Promoter - Pmlc-1 (w ATG)
Plasmid#159888PurposeThree-fragment Gateway compatible promoter for expression in all muscles of C. elegansDepositorInsert[4-1] - Promoter - Pmlc-1 (w ATG)
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSEM223 - [4-1] - Promoter - Pmlc-2(no_start)
Plasmid#159889PurposeThree-fragment Gateway compatible promoter for expression in all muscles of C. elegansDepositorInsert[4-1] - Promoter - Pmlc-2(no_start)
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAc-His-zfKitlgb
Plasmid#140293PurposeExpress soluble His-tagged zebrafish Kitlgb in insect cellsDepositorInsertKitlgb (kitlgb Zebrafish)
UseTags6x HisExpressionInsectMutationPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS416-PTEF1-MCP-5M-ECM33_intron
Plasmid#127600Purposelow-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoter, 5M-ECM33 intron inserted into URA3DepositorInsertECM33 intron with MS2 hairpins replacing sequence near 5′ end of intron
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
-
Myo 9 Avi+Flag
Plasmid#134969PurposeExpression and purification of c.elegans Myo 9 (Hum 7) using baculovirus from pFastBac1DepositorInsertMyo 9 (hum-7 Nematode)
UseTagsAvi and FlagExpressionInsectMutationPromoterPolyhedrinAvailable sinceJan. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMV306-ribo-Sec-APEX2
Plasmid#111701PurposeTheophylline-inducible expression of periplasmic APEX2 from a mycobacterial shuttle plasmid (integrating)DepositorInsertAPEX2
UseTagsV5 and mpt63 signal sequenceExpressionBacterialMutationPromoterhsp60-riboAvailable sinceMarch 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCK039
Plasmid#105425PurposeSynthetic biologyDepositorInsertdTALE (binding to Bs3 promotor (Capsicum annuum) as transactivator)
UseSynthetic BiologyTagsExpressionMutationBsaI/ BpiI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only