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Plasmid#62857PurposeSplicing reporter control (no intron) with elements to shorten the half-life of the luciferase protein as well as the luciferase mRNA.DepositorInsertLuciferase
UseLuciferaseTagsExpressionMammalianMutationdestabilizing sequences (DS) added to the C termi…PromoterCMVAvailable sinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
LRTi
Plasmid#214072PurposeLuminescent reporter system to measure EJC-enhanced NMD activity. No intron in 5'UTRDepositorInsertFirefly luciferase
UseTagsExpressionMammalianMutationLinker sequence present between firefly luciferas…PromoterAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
LRTX_ctrl
Plasmid#214073PurposeLuminescent reporter system, NMD negative control. No intron in 5'UTRDepositorInsertFirefly luciferase
UseTagsExpressionMammalianMutationRemnant of linker sequence present at the end of …PromoterAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
LRT_RTctrl
Plasmid#214074PurposeLuminescent reporter system, readthrough control, NMD negative control. No intron in 5'UTRDepositorInsertFirefly luciferase
UseTagsExpressionMammalianMutationLinker sequence present between firefly luciferas…PromoterAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
LRT
Plasmid#214071PurposeLuminescent reporter system to measure EJC-independent NMD activity. No intron in 5'UTRDepositorInsertFirefly luciferase
UseTagsExpressionMammalianMutationLinker sequence present between firefly luciferas…PromoterAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
LiRTX_ctrl
Plasmid#214077PurposeLuminescent reporter system, NMD negative control. Intron present in 5'UTRDepositorInsertFirefly luciferase
UseTagsExpressionMammalianMutationRemnant of linker sequence present at the end of …PromoterAvailable sinceFeb. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
MLRV
Plasmid#118069PurposeMulti-luciferase reporter vector including transcriptional reporters for NF-kb, TGF-b, c-Myc, p53 and MAPK/JNK transcriptional reportersDepositorInsertsTB:5xNF-κβ:RedF:bGHpA
TB:4xTGF-β:FLuc:bGHpA
TB:5xE-box:Renilla:bGHpA
TB:2xp53:NLuc:bGHpA
TB:6xAP-1:GrRenilla:bGHpA
CMV:ELuc:bGHpA
UseLuciferase and Synthetic Biology; Assembly of gb2…TagsExpressionMammalianMutationPromoter2xp53::miniP, 4xTGF-B::miniP, 5xEbox::miniP, 5xNF…Available sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCR3.1-mutLuc
Plasmid#106458Purposeluciferase reporter, CMV promoter (3 NFkB sites mutated)DepositorInsertluciferase reporter, CMV promoter (3 NFkB sites mutated)
UseLuciferaseTagsExpressionMammalianMutation3 NFkB sites mutatedPromoterAvailable sinceJuly 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUPD2+E-Luc
Plasmid#170873PurposePhytobrick - E-Luc Luciferase CDSDepositorInsertE-Luc
UseLuciferase and Synthetic BiologyTagsExpressionMutationc.375C>T to remove BbsI sitePromoterAvailable sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL3-pomMx1
Plasmid#30536PurposeReporter (luciferase) for interferon signalling in fishDepositorInsertrainbow trout Mx 1 promoter
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceMay 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
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LiRT
Plasmid#214075PurposeLuminescent reporter system to measure EJC-independent NMD activity. Intron present in 5'UTRDepositorInsertFirefly luciferase
UseTagsExpressionMammalianMutationLinker sequence present between firefly luciferas…PromoterAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
LiRT_RTctrl
Plasmid#214078PurposeLuminescent reporter system, readthrough control, NMD negative control. Intron present in 5'UTRDepositorInsertFirefly luciferase
UseTagsExpressionMammalianMutationLinker sequence present between firefly luciferas…PromoterAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
LiRTi
Plasmid#214076PurposeLuminescent reporter system to measure EJC-enhanced NMD activity. Intron present in 5'UTRDepositorInsertFirefly luciferase
UseTagsExpressionMammalianMutationLinker sequence present between firefly luciferas…PromoterAvailable sinceFeb. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDGB1a2R+pNOS_Red-F_NOSt
Plasmid#170888PurposeGoldenBraid Transcriptional Unit - pNOS_Red-F_NOSt Reverse orientationDepositorInsertRed-F
UseLuciferase and Synthetic BiologyTagsExpressionPlantMutationp.S286Y Red mutantPromoterpNOSAvailable sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDGB1a2+pNOS_Red-F_NOSt
Plasmid#170887PurposeGoldenBraid Transcriptional Unit - pNOS_Red-F_NOSt Forward orientationDepositorInsertRed-F
UseLuciferase and Synthetic BiologyTagsExpressionPlantMutationp.S286Y Red mutantPromoterpNOSAvailable sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTXTL-T7max-NanoLuc
Plasmid#188313PurposeExpression of NanoLuc via a T7 promoter in TXTLDepositorInsertNanoLuc
UseLuciferase and Synthetic BiologyTags8x His-tagExpressionBacterialMutationPromoterT7MaxAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTXTL-T7max-LuxA
Plasmid#188305PurposeExpression of LuxA via a T7 promoter in TXTLDepositorInsertluxA
UseLuciferase and Synthetic BiologyTagsExpressionBacterialMutationPromoterT7MaxAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTXTL-T7max-LuxB
Plasmid#188306PurposeExpression of LuxB via a T7 promoter in TXTLDepositorInsertluxB
UseLuciferase and Synthetic BiologyTagsExpressionBacterialMutationPromoterT7MaxAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCR3.1-CREM2 luc
Plasmid#106460Purposeluciferase reporter, CMV promoter (CRE site adjacent to NFkB site mutated)DepositorInsertluciferase reporter, CMV promoter (CRE site adjacent to NFkB site mutated)
UseLuciferaseTagsExpressionMammalianMutationCRE site adjacent to NFkB site mutatedPromoterAvailable sinceAug. 17, 2020AvailabilityAcademic Institutions and Nonprofits only