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Plasmid Kit#1000000094Purpose68 His-tagged human kinase catalytic domain constructs and the respective phosphatases that enhance their bacterial expression; optimized for automated bacterial expressionDepositorAvailable sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only
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GFP-Rab35 S22N inactive
Plasmid#47426DepositorInsertRab35 S22N (RAB35 Human)
UseTagsGFPExpressionMammalianMutationS22N inactivePromoterCMVAvailable sinceJuly 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
Cbh_v5 AAV-ABE C-terminal
Plasmid#137178PurposeAAV genome: expresses the C-terminal of v5 AAV-ABE from the Cbh promoter, and U6-sgRNADepositorInsertv5 AAV-CBE C-terminal; U6-protospacer
UseAAVTagsExpressionMutationPromoterCMVAvailable sinceJan. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
hSynapsin-PE2-Cterminal-lenti
Plasmid#135956PurposeNeuronal expression of C-terminal intein-split prime editor v2 (PE2). This plasmid is used by PE2, PE3, and PE3bDepositorInsertPrime editor v2 (PE2) C-terminal
UseLentiviralTagsExpressionMammalianMutationMMLV RT(D200N, T306K, W313F, T330P, L603W)PromoterhSynapsinAvailable sinceJan. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
Cbh_v5 AAV-CBE C-terminal
Plasmid#137176PurposeAAV genome: expresses the C-terminal of v5 AAV-CBE from the Cbh promoter, and U6-sgRNADepositorInsertv5 AAV-CBE C-terminal; U6-protospacer
UseAAVTagsExpressionMutationPromoterCbhAvailable sinceJan. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
BirA(R118G)-HA destination vector
Plasmid#53581PurposeTo fuse your protein of interest to the N-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID)DepositorTypeEmpty backboneUseTagsBirA(R118G) and HA tagExpressionMammalianMutationPromoterCMVAvailable sinceJune 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
Cbh_v5 AAV-ABE N-terminal
Plasmid#137177PurposeAAV genome: expresses the N-terminal of v5 AAV-ABE from the Cbh promoterDepositorInsertv5 AAV-ABE N-terminal
UseAAVTagsExpressionMutationCas9 D10APromoterCbhAvailable sinceJan. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
T7 promoter + terminators reporter plasmid
Plasmid#156456PurposeA mutational reporter plasmid that can gain Kanamycin and/or Tetracycline resistance upon mutation. Contains T7 promoter and a terminator array.DepositorInsertsNeoR/KanR
TcR
UseTagsExpressionBacterialMutationATG start codon mutated to ACGPromoterEm7 and tetAvailable sinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-neo-cterminal-3HA
Plasmid#102643PurposeTo generate three copies of the HA epitope tag on cDNAs.DepositorTypeEmpty backboneUseTags3x HA (YPYDVPDYA)ExpressionMammalianMutationPromoterAvailable sinceOct. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
myc mTOR kinase dead
Plasmid#8482DepositorInsertmTOR (Mtor Rat)
UseTagsmycExpressionMammalianMutationD2357E V2364IPromoterAvailable sinceJune 20, 2005AvailabilityAcademic Institutions and Nonprofits only -
Cbh_v5 AAV-CBE N-terminal
Plasmid#137175PurposeAAV genome: expresses the N-terminal of v5 AAV-CBE from the Cbh promoter, and U6-sgRNADepositorInsertv5 AAV-CBE N-terminal; U6-protospacer
UseAAVTagsExpressionMutationCas9 D10APromoterCbhAvailable sinceJan. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ATR Kinase dead
Plasmid#31612DepositorInsertATR (ATR Human)
UseTagsFlagExpressionMammalianMutationK2327R (kinase dead)PromoterAvailable sinceNov. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCMV-dCas13-inactive M3nls
Plasmid#157854PurposeTargeted m6A RNA methylation in mammalian cells, methyltransferase-inactive mutantDepositorInsertdCas13-inactive dCas13-inactive M3nls (METTL3 Cas13b is from Prevotella sp. P5-125; METTL3 is from H. sapiens)
UseTagsExpressionMammalianMutationPspCas13b Δ984-1090 H133A; METTL3 D395APromoterAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
Dominant negative Hsp90 beta
Plasmid#22480DepositorInsertDominant negative Hsp90 (HSP90AB1 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceDec. 8, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCMV-dCas13-inactive M3M14nes
Plasmid#157855PurposeTargeted m6A RNA methylation in mammalian cells, methyltransferase-inactive mutantDepositorInsertUseTagsExpressionMammalianMutationPspCas13b Δ984-1090 H133A; METTL3 D395APromoterAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-smURFP-T2A-mCardinal
Plasmid#80348PurposeLentiviral transfer vector under the CMV promoter that expresses smURFP and mCardinal.DepositorInsertssmURFP
mCardinal
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAG-T7-TALEN(Sangamo)-Destination
Plasmid#37184PurposeDestination vector for Voytas Golden Gate TALEN assembly incorporating a CAG promoter for mammalian expression and a T7 promoter for synthesis of TALEN mRNA. Uses homodimeric FokI domains.DepositorTypeEmpty backboneUseTagsFokI nucleaseExpressionMammalianMutationPromoterCAGAvailable sinceJuly 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
Cbh_v5 AAV-saCBE N-terminal
Plasmid#137182PurposeAAV genome: expresses the N-terminal of S. aureus v5 AAV-CBE from the Cbh promoter, U6-sgRNADepositorInsertv5 AAV-saCBE N-terminal
UseAAVTagsExpressionMutationCas9 D10APromoterCbhAvailable sinceFeb. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
Cbh_v5 AAV-saCBE C-terminal
Plasmid#137183PurposeAAV genome: expresses the C-terminal of S. aureus v5 AAV-CBE from the Cbh promoter, and U6-sgRNADepositorInsertv5 AAV-saCBE C-terminal
UseAAVTagsExpressionMutationPromoterCbhAvailable sinceJan. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-U6-pegSERPINA1-U6-Nicking-PE2-N
Plasmid#164907PurposeExpress a pegRNA targeting SERPINA1, and a nicking sgRNA and the N-terminal split-intein fragment of PE2DepositorInsertpegSERPINA1, Nicking sgRNA, PE2-N
UseAAVTagsExpressionMutationPromoterAvailable sinceFeb. 8, 2021AvailabilityAcademic Institutions and Nonprofits only